The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates.
Open accessAbstract
A target length limitation to PCR amplification of DNA has been identified and addressed. Concomitantly, the base-pair fidelity, the ability to use PCR products as primers, and the maximum yield of target fragment were increased. These improvements were achieved by the combination of a high level of an exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase, Klentaq1, with a very low level of a thermostable DNA polymerase exhibiting a 3'-exonuclease activity (Pfu, Vent, or Deep Vent). At least 35 kb can be amplified to high yields from 1 ng of lambda DNA template.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
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Marsischky, G. T., Filosi, N., Kane, M. F., & Kolodner, R. (1996) · Genes & Development · doi:10.1101/gad.10.4.407 · Cited by 581
RuvAB Acts at Arrested Replication Forks
Seigneur, M., Bidnenko, V., Ehrlich, S. D., & Michel, B. (1998) · Cell · doi:10.1016/s0092-8674(00)81772-9 · Cited by 538
Normalization and subtraction: two approaches to facilitate gene discovery.
Bonaldo, M. F., Lennon, G., & Soares, M. B. (1996) · Genome Research · doi:10.1101/gr.6.9.791 · Cited by 522
Günther, S., Li, B. C., Miska, S., Krüger, D. H., Meisel, H., & Will, H. (1995) · Journal of Virology · doi:10.1128/jvi.69.9.5437-5444.1995 · Cited by 499
Rawson, R. B., Zelenski, N. G., Nijhawan, D., Ye, J., Sakai, J., Hasan, M. T., Chang, T. Y., Brown, M. S., & Goldstein, J. L. (1997) · Molecular Cell · doi:10.1016/s1097-2765(00)80006-4 · Cited by 429
Metadata
- Authors
- Barnes, W. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1994-03-15
- Volume
- 91
- Issue
- 6
- Pages
- 2216-2220
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1987666378
- Cited by
- 1,034
- References
- 2
- FWCI
- 35.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Barnes, W. M. (1994). PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.91.6.2216
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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