The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
A novel method for efficient amplification of whole hepatitis B virus genomes permits rapid functional analysis and reveals deletion mutants in immunosuppressed patients
Open accessAbstract
Current knowledge of hepatitis B virus (HBV) sequence heterogeneity is based mainly on sequencing of amplified subgenomic HBV fragments. Here, we describe a method which allows sensitive amplification and simplified functional analysis of full-length HBV genomes with or without prior cloning. By this method, a large number of HBV genomes were cloned from sera of six immunosuppressed kidney transplant patients. Two size classes of HBV genomes, one 3.2 kb and another about 2.0 kb in size, were found in all patients. The genome population from one serum sample was studied in detail by size analysis of subgenomic PCR fragments and sequencing. Regions with deletions and insertions were mapped in the C gene and pre-S region. Up to 100% of HBV genomes in all other immunosuppressed patients also had deletions in the C gene. Our results demonstrate the potential of the established method for the structural and functional characterization of heterogeneous populations of complete virion-encapsidated HBV DNAs and suggest that HBV genomes with C gene deletions can have a selective advantage in immunosuppressed patients.
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Cites
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Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli
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Self-citationWakita, T., Kakumu, S., Shibata, M., Yoshioka, K., Ito, Y. L. S., Shinagawa, T., Ishikawa, T., Takayanagi, M., & Morishima, T. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115500 · Cited by 95
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Cited by
Control of cccDNA function in hepatitis B virus infection
Levrero, M., Pollicino, T., Petersen, J., Belloni, L., Raimondo, G., & Dandri, M. (2009) · Journal of Hepatology · doi:10.1016/j.jhep.2009.05.022 · Cited by 552
Murakami, Y., Saigo, K., Takashima, H., Minami, M., Okanoue, T., Bréchot, C., & Paterlini-Bréchot, P. (2005) · Gut · doi:10.1136/gut.2004.054452 · Cited by 331
Dandri, M., Burda, M. R., Will, H., & Petersen, J. (2000) · Hepatology · doi:10.1053/jhep.2000.8701 · Cited by 106
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Metadata
- Authors
- Günther, S., Li, B. C., Miska, S., Krüger, D. H., Meisel, H., & Will, H.
- Venue
- Journal of Virology
- Publisher
- American Society for Microbiology
- Published
- 1995-09
- Volume
- 69
- Issue
- 9
- Pages
- 5437-5444
- ISSN
- 0022-538X (ISSN-L)
- 1098-5514
- OpenAlex
- W2156552801
- Cited by
- 499
- References
- 9
- FWCI
- 5.63
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Günther, S., Li, B. C., Miska, S., Krüger, D. H., Meisel, H., & Will, H. (1995). A novel method for efficient amplification of whole hepatitis B virus genomes permits rapid functional analysis and reveals deletion mutants in immunosuppressed patients. Journal of Virology. https://doi.org/10.1128/jvi.69.9.5437-5444.1995
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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