Platelet-derived growth factor stimulates the secretion of hyaluronic acid by proliferating human vascular smooth muscle cells.
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Total glycans from the cell layer and the culture medium of human vascular smooth muscle cells (VSMC) that had been cultivated in the presence of platelet-derived growth factor (PDGF) were isolated and purified by gel filtration after Pronase and DNase digestion and alkaliborohydride treatment. Measurements of the content of neutral hexoses and uronic acids revealed that PDGF stimulates total glycan synthesis by proliferating VSMC in a linear fashion from 24 h to 72 h of incubation. In contrast, total glycan synthesis by human fibroblasts, epithelial cells, or endothelial cells was not affected by PDGF, indicating cell-type specificity. Chemical, biochemical, and enzymological characterization of the total glycans synthesized by VSMC showed that PDGF stimulates the secretion of a 340-kDa glycan molecule in a time-dependent manner from 24 h to 72 h. This molecule is highly acidic, shares a common structure with hyaluronic acid, and exhibits a potent antiproliferative activity on VSMC. These results suggest that VSMC in response to PDGF are capable of controlling their own growth and migration by the synthesis of a specific form of hyaluronic acid with antiproliferative potency, which may be involved in the regulation of the local inflammatory responses associated with atherosclerosis.
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- Authors
- Papakonstantinou, E., Karakiulakis, G., Roth, M., & Block, L. H.
- Affiliations
- University of Vienna (AT)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1995-10-10
- Volume
- 92
- Issue
- 21
- Pages
- 9881-9885
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC40906
- OpenAlex
- W2008709628
- Cited by
- 85
- References
- 26
- Citations by year
- 2026: 1 · 2023: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2017: 1 · 2016: 5 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 1.49
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- platelet-derived growth factor, hyaluronic acid, vascular smooth muscle cells, glycan synthesis, cell proliferation, extracellular matrix secretion, uronic acid, antiproliferative activity, cell migration, atherosclerosis, cell type specificity
- MeSH terms
- Amino Acids, Cell Division, Cells, Cultured, Chromatography, High Pressure Liquid, Fibroblasts, Humans, Hyaluronic Acid, Lung, Muscle, Smooth, Vascular, Platelet-Derived Growth Factor, Polysaccharide-Lyases, Polysaccharides, Muscle Development
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