Localization of PDGF-B Protein in Macrophages in All Phases of Atherogenesis
Abstract
Lesions of atherosclerosis occur in the innermost layer of the artery wall and consist primarily of proliferated smooth muscle cells surrounded by large amounts of connective tissue, numerous lipid-laden macrophages, and varying numbers of lymphocytes. Growth-regulatory molecules may be involved in intimal accumulation and proliferation of smooth muscle cells responsible for the occlusive lesions of atherosclerosis. Platelet-derived growth factor (PDGF) B-chain protein was found within macrophages in all stages of lesion development in both human and nonhuman primate atherosclerosis. Thus macrophages may play a critical role in the disease by providing PDGF, a potent chemotactic and growth-stimulatory molecule, to the intimal smooth muscle cells.
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In mice, fibrous cap VSMCs are derived from medial VSMCs that migrated and proliferated in response to cytokines and growth factors such as PDGF from macrophages and activated ECs.
“In mice, the fibrous cap VSMCs are derived from medial VSMCs 22,138 that have undergone migration and proliferation in response to cytokines and growth factors, such as PDGF, derived from macrophages and activated ECs 23,129,139 .”
From Vascular smooth muscle cells in atherosclerosis (2019) · cites this paper as [129]
Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczyński, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 47,092 · see citing works
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
Feinberg, A. P., & Vogelstein, B. (1983) · Analytical Biochemistry · doi:10.1016/0003-2697(83)90418-9 · Cited by 24,512 · see citing works
Melton, D. A., Krieg, P. A., Rebagliati, M. R., Maniatis, T., Zinn, K., & Green, M. R. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.18.7035 · Cited by 6,324 · see citing works
The Pathogenesis of Atherosclerosis — An Update
Ross, R. (1986) · New England Journal of Medicine · doi:10.1056/nejm198602203140806 · Cited by 4,885 · see citing works
Self-citationSerum cholesterol, lipoproteins, and the risk of coronary heart disease. The Framingham study.
Kannel, W., Castelli, W., Gordon, T., & McNamara, P. M. (2020) · Annals of Internal Medicine · doi:10.7326/0003-4819-74-1-1 · Cited by 2,160 · see citing works
The biology of platelet-derived growth factor
Ross, R., Raines, E. W., & Bowen‐Pope, D. F. (1986) · Cell · doi:10.1016/0092-8674(86)90733-6 · Cited by 2,023 · see citing works
Self-citationThe Pathogenesis of Atherosclerosis
Ross, R., & Glomset, J. A. (1976) · New England Journal of Medicine · doi:10.1056/nejm197608122950707 · Cited by 2,021 · see citing works
Self-citationDerynck, R., Jarrett, J. A., Chen, E. Y., Eaton, D. H., BELL, J. R., Assoian, R. K., Roberts, A. B., Sporn, M. B., & Goeddel, D. V. (1985) · Nature · doi:10.1038/316701a0 · Cited by 1,667 · see citing works
Atherosclerosis and the Arterial Smooth Muscle Cell
Ross, R., & Glomset, J. A. (1973) · Science · doi:10.1126/science.180.4093.1332 · Cited by 1,504 · see citing works
Self-citationYarden, Y., Escobedo, J. A., Kuang, W.-J., Yang‐Feng, T. L., Daniel, T. O., Tremble, P., Chen, E. Y., Ando, M. E., Harkins, R. N., Francke, U., et al. (1986) · Nature · doi:10.1038/323226a0 · Cited by 1,208 · see citing works
Faggiotto, A., Ross, R., & Harker, L. A. (1984) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.4.4.323 · Cited by 990 · see citing works
Self-citationHyperlipidemia and Atherosclerosis
Ross, R., & Harker, L. A. (1976) · Science · doi:10.1126/science.822515 · Cited by 762 · see citing works
Self-citationA significant part of macrophage-derived growth factor consists of at least two forms of PDGF
Shimokado, K., Raines, E. W., Madtes, D. K., Barrett, T. B., Benditt, E. P., & Ross, R. (1985) · Cell · doi:10.1016/0092-8674(85)90033-9 · Cited by 715 · see citing works
Self-citationGown, A. M., Tsukada, T., & Ross, R. (1986) · PubMed · Cited by 678 · see citing works
Self-citationInterleukin-1 Mitogenic Activity for Fibroblasts and Smooth Muscle Cells Is Due to PDGF-AA
Raines, E. W., Dower, S., & Ross, R. (1989) · Science · doi:10.1126/science.2783498 · Cited by 642 · see citing works
Self-citationTwo Different Subunits Associate to Create Isoform-Specific Platelet-derived Growth Factor Receptors
Seifert, R. A., Hart, C. E., Phillips, P. E., Forstrom, J. W., Ross, R., Murray, M. J., & Bowen‐Pope, D. F. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81860-9 · Cited by 610 · see citing works
Self-citationIsolation of a Novel Receptor cDNA Establishes the Existence of Two PDGF Receptor Genes
Matsui, T., Heidaran, M. A., Miki, T., Popescu, N. C., Rochelle, W. L., Kraus, M. H., Pierce, J. H., & Aaronson, S. A. (1989) · Science · doi:10.1126/science.2536956 · Cited by 596 · see citing works
Two Classes of PDGF Receptor Eecognize Different Isoforms of PDGF
Hart, C. E., Forstrom, J. W., Kelly, J. D., Seifert, R. A., Smith, R. A., Ross, R., Murray, M. J., & Bowen‐Pope, D. F. (1988) · Science · doi:10.1126/science.2836952 · Cited by 580 · see citing works
Self-citationImmunogold-silver staining: new method of immunostaining with enhanced sensitivity.
Holgate, C. S., Jackson, P. K., Cowen, P. J., et al. (1983) · Journal of Histochemistry & Cytochemistry · doi:10.1177/31.7.6189883 · Cited by 558 · see citing works
Heldin, C. H., Bäckström, G., Östman, A., Hammacher, A., Rönnstrand, L., Rubin, K., Nistér, M., & Westermark, B. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02955.x · Cited by 498 · see citing works
Wilcox, J. N., Smith, K. M., Williams, L. T., Schwartz, S. M., & Gordon, D. (1988) · Journal of Clinical Investigation · doi:10.1172/jci113671 · Cited by 476 · see citing works
Faggiotto, A., & Ross, R. (1984) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.4.4.341 · Cited by 465 · see citing works
Self-citationThe role of the monocyte in atherogenesis: II. Migration of foam cells from atherosclerotic lesions.
Gerrity, R. G. (1981) · PubMed · Cited by 459 · see citing works
Structural alteration of viral homologue of receptor proto-oncogene fms at carboxyl terminus
Coussens, L. M., Beveren, C. V., Smith, D. H., Chen, E., Mitchell, R. L., Isacke, C. M., Verma, I. M., & Ullrich, A. (1986) · Nature · doi:10.1038/320277a0 · Cited by 447 · see citing works
Heldin, C., Westermark, B., & Wasteson, Å. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.6.3664 · Cited by 427 · see citing works
Platelet-derived growth factor. II. Specific binding to cultured cells.
Bowen‐Pope, D. F., & Ross, R. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34650-7 · Cited by 366 · see citing works
Self-citationHeldin, C., Ernlund, A., Rorsman, C., & Rönnstrand, L. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)81879-8 · Cited by 349 · see citing works
Mosley, B., Urdal, D. L., Prickett, K. S., Larsen, A., Cosman, D. J., Conlon, P. J., Gillis, S. A., & Dower, S. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)61450-4 · Cited by 343 · see citing works
Rosenfeld, M. E., Tsukada, T., Gown, A. M., & Ross, R. (1987) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.7.1.9 · Cited by 330 · see citing works
Self-citationGronwald, R. G., Grant, F. J., Haldeman, B. A., Hart, C. E., O’Hara, P. J., Hagen, F. S., Ross, R., Bowen‐Pope, D. F., & Murray, M. J. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.10.3435 · Cited by 316 · see citing works
Self-citationCollins, T., Ginsburg, D., Boss, J. M., Orkin, S. H., & Pober, J. S. (1985) · Nature · doi:10.1038/316748a0 · Cited by 285 · see citing works
Barraclough, B. M. (1976) · The Lancet · doi:10.1016/s0140-6736(76)92691-x · Cited by 279 · see citing works
Frank, R. T. (1949) · American Journal of Obstetrics and Gynecology · doi:10.1016/0002-9378(49)90351-8 · Cited by 272 · see citing works
Poole, J. C. F., & Florey, H. W. (1958) · The Journal of Pathology and Bacteriology · doi:10.1002/path.1700750202 · Cited by 266 · see citing works
Fish Oils Inhibit Endothelial Cell Production of Platelet-Derived Growth Factor-Like Protein
Fox, P. L., & DiCorleto, P. E. (1988) · Science · doi:10.1126/science.3393911 · Cited by 265 · see citing works
Barrett, T. B., & Benditt, E. P. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.8.2810 · Cited by 242 · see citing works
Rosenfeld, M. E., Tsukada, T., Chait, A., Bierman, E. L., Gown, A. M., & Ross, R. (1987) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.7.1.24 · Cited by 175 · see citing works
Self-citationMasuda, J., & Ross, R. (1990) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.10.2.164 · Cited by 167 · see citing works
Self-citationDegen, J. L., Neubauer, M., Degen, S. J., et al. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44150-0 · Cited by 160 · see citing works
Florentin, R. A., Nam, S. C., Daoud, A. S., et al. (1968) · Experimental and Molecular Pathology · doi:10.1016/s0014-4800(68)80001-2 · Cited by 143 · see citing works
[8] methods for studying the platelet-derived growth factor receptor
Bowen‐Pope, D. F., & Ross, R. (1985) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(85)09078-4 · Cited by 117 · see citing works
Self-citationMasuda, J., & Ross, R. (1990) · Arteriosclerosis An Official Journal of the American Heart Association Inc · doi:10.1161/01.atv.10.2.178 · Cited by 115 · see citing works
Self-citationThe genesis of atherosclerosis
POLLACK, H. (1941) · American Heart Journal · doi:10.1016/s0002-8703(41)90783-4 · Cited by 93 · see citing works
Fox, P. L., Chisolm, G. M., & DiCorleto, P. E. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)45535-4 · Cited by 86 · see citing works
Fox, P. L., & DiCorleto, P. E. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.13.4774 · Cited by 62 · see citing works
SHIRAISHI, T., Morimoto, S., Itoh, K., et al. (1989) · Clinica Chimica Acta · doi:10.1016/0009-8981(89)90257-x · Cited by 55 · see citing works
An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry
Ormanns, W., & Schäffer, R. (1985) · Histochemistry and Cell Biology · doi:10.1007/bf02450475 · Cited by 18 · see citing works
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Vascular endothelial growth factor enhances atherosclerotic plaque progression
Celletti, F. L., Waugh, J. M., Amabile, P. G., Brendolan, A., Hilfiker, P. R., & Dake, M. D. (2001) · Nature Medicine · doi:10.1038/86490 · Cited by 708 · see citing works
Hypoxia Regulates Macrophage Functions in Inflammation
Murdoch, C., Muthana, M., & Lewis, C. E. (2005) · The Journal of Immunology · doi:10.4049/jimmunol.175.10.6257 · Cited by 455 · see citing works
Yamakage, A., Kikuchi, K., Smith, E. A., LeRoy, E. C., & Trojanowska, M. (1992) · The Journal of experimental medicine · doi:10.1084/jem.175.5.1227 · Cited by 184 · see citing works
Grappone, C., Pinzani, M., Parola, M., Pellegrini, G., Caligiuri, A., DeFranco, R., Marra, F., Herbst, H., Alpini, G., & Milani, S. (1999) · Journal of Hepatology · doi:10.1016/s0168-8278(99)80169-x · Cited by 133 · see citing works
Vascular transcellular signaling.
Marcus, A. J., & Hajjar, D. P. (1993) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)35344-x · Cited by 114 · see citing works
Growth factor and procollagen type I gene expression in human liver disease
Malizia, G., Brunt, E. M., Peters, M. G., Rizzo, A., Broekelmann, T. J., & McDonald, J. A. (1995) · Gastroenterology · doi:10.1016/0016-5085(95)90019-5 · Cited by 89 · see citing works
Papakonstantinou, E., Karakiulakis, G., Roth, M., & Block, L. H. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.21.9881 · Cited by 85 · see citing works
- Authors
- Ross, R., Masuda, J., Raines, E. W., Gown, A. M., Katsuda, S., Sasahara, M., Malden, L. T., Masuko, H., & Sato, H.
- Affiliations
- University of Washington (US)
- National Cerebral and Cardiovascular Center (JP)
- Mochida Pharmaceutical (Japan) (JP)
- Venue
- Science
- Published
- 1990-05-25
- Volume
- 248
- Issue
- 4958
- Pages
- 1009-1012
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1974790290
- Cited by
- 574
- References
- 49
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 5 · 2020: 6 · 2019: 3 · 2018: 4 · 2017: 1 · 2016: 3 · 2015: 5 · 2014: 8 · 2013: 4 · 2012: 6
- FWCI
- 25.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01hl029873-05)
- National Heart, Lung, and Blood Institute (HL-18645, HL29873, P01HL018645)
- National Center for Research Resources (RR00166, P51RR000166)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cerebrovascular and Carotid Artery Diseases
- Keywords
- platelet-derived growth factor B-chain, macrophages, atherosclerosis, atherogenesis, smooth muscle cell proliferation, intimal thickening, lipid-laden macrophages, growth factors, chemotaxis, non-human primates
- MeSH terms
- Animals, Antibodies, Monoclonal, Arteriosclerosis, Diet, Atherogenic, Humans, Immunohistochemistry, Macaca nemestrina, Macrophages, Monocytes, Platelet-Derived Growth Factor, RNA, Messenger, Blotting, Northern
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