The DOI matches its Crossref record; 40 of the 41 references it cites were found in a registry or an index.
A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria.
Open accessAbstract
A novel Saccharomyces cerevisiae mutant, unable to grow in the presence of 12.5 mM EGTA, was isolated by replica plating. The phenotype of the mutant is caused by a single amino acid change (Gly149 to Arg) in the essential yeast gene CDC1. The mutant could be suppressed by overexpression of the SMF1 gene, which was isolated as an extragenic high-copy suppressor. The SMF1 gene codes for a highly hydrophobic protein and its deletion renders the yeast cells sensitive to low manganese concentration. In accordance with this observation, the smf1 null mutant exhibits reduced Mn2+ uptake at micromolar concentrations. Using a specific antibody, we demonstrated that Smf1p is located in the yeast plasma membrane. These results suggest that Smf1p is involved in high-affinity Mn2+ uptake. This assumption was also tested by overexpressing the SMF1 gene in the temperature-sensitive mutant of the mitochondrial processing peptidase (MAS1). SMF1 overexpression as well as addition of 1 mM Mn2+ to the growth medium complemented this mutation. This also suggests that in vivo Mas1p is a manganese-dependent peptidase. The yeast Smf1p resembles a protein from Drosophila and mammalian macrophages. The latter was implicated in conferring resistance to mycobacteria. A connection between Mn2+ transport and resistance or sensitivity to mycobacteria is discussed.
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“Homologous proteins in yeast, 63 flies, 64 and zebrafish 65 also transport iron and similar divalent cations.”
From Forging a field: the golden age of iron biology. (2008) · cites this paper as [63]
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DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518
Sherman, F. (1991) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(91)94004-v · Cited by 4,078
Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
Rothstein, R. (1991) · Methods in Enzymology · doi:10.1016/0076-6879(91)94022-5 · Cited by 1,283
Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg
Vidal, S. M., Malo, D., Vogan, K., Skamene, E., & Gros, P. (1993) · Cell · doi:10.1016/0092-8674(93)90135-d · Cited by 1,104
ZINC PROTEINS: Enzymes, Storage Proteins, Transcription Factors, and Replication Proteins
Coleman, J. E. (1992) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.61.070192.004341 · Cited by 980
The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake.
Askwith, C., Eide, D., Van Ho, A., Bernard, P. S., Li, L., Davis-Kaplan, S., Sipe, D. M., & Kaplan, J. (1994) · Cell · doi:10.1016/0092-8674(94)90346-8 · Cited by 670
Dancis, A., Yuan, D. S., Haile, D., Askwith, C., Eide, D., Moehle, C., Kaplan, J., & Klausner, R. D. (1994) · Cell · doi:10.1016/0092-8674(94)90345-x · Cited by 659
Goud, B., Salminen, A., Walworth, N. C., & Novick, P. (1988) · Cell · doi:10.1016/0092-8674(88)90093-1 · Cited by 585
The yeast secretory pathway is perturbed by mutations in PMR1, a member of a Ca2+ ATPase family
Rudolph, H. K., Antebi, A., Fink, G. R., Buckley, C. M. F., Dorman, T. E., LeVitre, J., Davidow, L. S., Mao, J.-I., & Moir, D. T. (1989) · Cell · doi:10.1016/0092-8674(89)90410-8 · Cited by 536
Research works printed but not linked: 4
- [8]Rooney, E. K. & Gross, J. D. (1992) Proc. Natl. Acad. Sci. USA 89, 8025-8029.
- [13]Noumi, T., Beltrán, C., Nelson, H. & Nelson, N. (1991) Proc. Natl. Acad. Sci. USA 88, 1938–1942.
- [17]Maniatis, T., Fritsch, E. F. & Sambrook, J. (1984) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 88–148.
- [19]Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463–5467.
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Metadata
- Authors
- Supek, F., Supekova, L., Nelson, H., & Nelson, N.
- Affiliations
- Roche Institute of Molecular Biology, Nutley, NJ 07110, USA.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1996-05-14
- Volume
- 93
- Issue
- 10
- Pages
- 5105-5110
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC39414
- OpenAlex
- W2015326313
- Cited by
- 325
- References
- 41
- Citations by year
- 2026: 1 · 2024: 4 · 2023: 7 · 2022: 10 · 2021: 11 · 2020: 9 · 2019: 8 · 2018: 6 · 2017: 8 · 2016: 3 · 2015: 10 · 2014: 9 · 2013: 5 · 2012: 5
- FWCI
- 10.74
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- manganese transporter, manganese deficiency, plasma membrane proteins, mitochondrial processing peptidase, Mas receptor, extragenic suppressors, Saccharomyces cerevisiae mutants
- MeSH terms
- Mitochondrial Processing Peptidase, Amino Acid Sequence, Animals, Biological Transport, Active, Carrier Proteins, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Macrophages, Manganese, Membrane Proteins, Metalloendopeptidases, Molecular Sequence Data, Mycobacterium, Phenotype, Saccharomyces cerevisiae, Point Mutation
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