CoChat
Sign Up
Cited by 325Fungal and yeast genetics researchHeavy Metal Exposure and ToxicityTrace Elements in Health

The DOI matches its Crossref record; 40 of the 41 references it cites were found in a registry or an index.

A yeast manganese transporter related to the macrophage protein involved in conferring resistance to mycobacteria.

Open access
Supek, F, Supekova, L, Nelson, H, Nelson, N·1996·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.93.10.5105PMC PMC39414
Open this paper in CoChat — free account

Abstract

A novel Saccharomyces cerevisiae mutant, unable to grow in the presence of 12.5 mM EGTA, was isolated by replica plating. The phenotype of the mutant is caused by a single amino acid change (Gly149 to Arg) in the essential yeast gene CDC1. The mutant could be suppressed by overexpression of the SMF1 gene, which was isolated as an extragenic high-copy suppressor. The SMF1 gene codes for a highly hydrophobic protein and its deletion renders the yeast cells sensitive to low manganese concentration. In accordance with this observation, the smf1 null mutant exhibits reduced Mn2+ uptake at micromolar concentrations. Using a specific antibody, we demonstrated that Smf1p is located in the yeast plasma membrane. These results suggest that Smf1p is involved in high-affinity Mn2+ uptake. This assumption was also tested by overexpressing the SMF1 gene in the temperature-sensitive mutant of the mitochondrial processing peptidase (MAS1). SMF1 overexpression as well as addition of 1 mM Mn2+ to the growth medium complemented this mutation. This also suggests that in vivo Mas1p is a manganese-dependent peptidase. The yeast Smf1p resembles a protein from Drosophila and mammalian macrophages. The latter was implicated in conferring resistance to mycobacteria. A connection between Mn2+ transport and resistance or sensitivity to mycobacteria is discussed.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1073/pnas.93.10.5105

Cites

40 of 40 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 41 references

Research works printed but not linked: 4

  1. [8]Rooney, E. K. & Gross, J. D. (1992) Proc. Natl. Acad. Sci. USA 89, 8025-8029.
  2. [13]Noumi, T., Beltrán, C., Nelson, H. & Nelson, N. (1991) Proc. Natl. Acad. Sci. USA 88, 1938–1942.
  3. [17]Maniatis, T., Fritsch, E. F. & Sambrook, J. (1984) Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 88–148.
  4. [19]Sanger, F., Nicklen, S. & Coulson, A. R. (1977) Proc. Natl. Acad. Sci. USA 74, 5463–5467.

Cited by

Sorted by citations

Related

See all

Metadata

Authors
Supek, F., Supekova, L., Nelson, H., & Nelson, N.
Affiliations
Roche Institute of Molecular Biology, Nutley, NJ 07110, USA.
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1996-05-14
Volume
93
Issue
10
Pages
5105-5110
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.93.10.5105
PMC
PMC39414
OpenAlex
W2015326313
Cited by
325
References
41
Citations by year
2026: 1 · 2024: 4 · 2023: 7 · 2022: 10 · 2021: 11 · 2020: 9 · 2019: 8 · 2018: 6 · 2017: 8 · 2016: 3 · 2015: 10 · 2014: 9 · 2013: 5 · 2012: 5
FWCI
10.74
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
  • Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
Keywords
manganese transporter, manganese deficiency, plasma membrane proteins, mitochondrial processing peptidase, Mas receptor, extragenic suppressors, Saccharomyces cerevisiae mutants
MeSH terms
Mitochondrial Processing Peptidase, Amino Acid Sequence, Animals, Biological Transport, Active, Carrier Proteins, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Macrophages, Manganese, Membrane Proteins, Metalloendopeptidases, Molecular Sequence Data, Mycobacterium, Phenotype, Saccharomyces cerevisiae, Point Mutation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review