Cited by 634RNA Interference and Gene DeliveryLipid Membrane Structure and BehaviorNanoparticle-Based Drug Delivery

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Mechanism of oligonucleotide release from cationic liposomes.

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Zelphati, O, Szoka, F C·1996·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.93.21.11493PMC PMC38085

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Abstract

We propose a mechanism for oligonucleotide (ODN) release from cationic lipid complexes in cells that accounts for various observations on cationic lipid-nucleic acid-cell interactions. Fluorescent confocal microscopy of cells treated with rhodamine-labeled cationic liposome/ fluorescein-labeled ODN (F-ODN) complexes show the F-ODN separates from the lipid after internalization and enters the nucleus leaving the fluorescent lipid in cytoplasmic structures. ODN displacement from the complex was studied by fluorescent resonance energy transfer. Anionic liposome compositions (e.g., phosphatidylserine) that mimic the cytoplasmic facing monolayer of the cell membrane released ODN from the complex at about a 1:1 (-/+) charge ratio. Release was independent of ionic strength and pH. Physical separation of the F-ODN from monovalent and multivalent cationic lipids was confirmed by gel electrophoresis. Fluid but not solid phase anionic liposomes are required, whereas the physical state of the cationic lipids does not effect the release. Water soluble molecules with a high negative linear charge density, dextran sulfate, or heparin also release ODN. However, ATP, spermidine, spermine, tRNA, DNA, polyglutamic acid, polylysine, bovine serum albumin, or histone did not release ODN, even at 100-fold charge excess (-/+). Based upon these results, we propose that the complex, after internalization by endocytosis, induces flip-flop of anionic lipids from the cytoplasmic facing monolayer. Anionic lipids laterally diffuse into the complex and form a charged neutralized ion-pair with the cationic lipids. This leads to displacement of the ODN from the cationic lipid and its release into the cytoplasm.

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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954
Cellular and Molecular Barriers to Gene Transfer by a Cationic Lipid
Zabner, J., Fasbender, A., Moninger, T., Poellinger, K. A., & Welsh, M. J. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.32.18997 · Cited by 1,412
Cationic liposome-mediated transfection
Felgner, P. L., & RINGOLD, G. M. (1989) · Nature · doi:10.1038/337387a0 · Cited by 989
Cationic liposome-mediated RNA transfection.
Malone, R. W., Felgner, P. L., & Verma, I. M. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6077 · Cited by 911
Efficient gene transfer into mammalian primary endocrine cells with lipopolyamine-coated DNA.
Behr, J., DEMENEIX, B. A., Loeffler, J.-P., & Pérez-Mutul, J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.18.6982 · Cited by 719
Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides.
Bennett, C. F., Chiang, M. Y., Chan, H. E. H., Shoemaker, J. E., & Mirabelli, C. K. (1992) · Molecular Pharmacology · doi:10.1016/s0026-895x(25)09229-6 · Cited by 612
DNA transfection mediated by cationic liposomes containing lipopolylysine: characterization and mechanism of action
Zhou, X., & Huang, L. (1994) · Biochimica et Biophysica Acta (BBA) - Biomembranes · doi:10.1016/0005-2736(94)90066-3 · Cited by 501
Antisense oligonucleotides inhibit intercellular adhesion molecule 1 expression by two distinct mechanisms.
Chiang, M. Y.-N., Chan, H., Zounes, M. A., Freier, S., Lima, W. F., & Bennett, C. F. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55250-9 · Cited by 463
Structure and Biological Activity of Heparin
Casu, B. (1985) · Advances in carbohydrate chemistry and biochemistry · doi:10.1016/s0065-2318(08)60067-0 · Cited by 429
A new cationic liposome reagent mediating nearly quantitative transfection of animal cells.
Rose, J. K., Buonocore, L., & Whitt, M. A. (1991) · PubMed · Cited by 385

Show all 22 references

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Authors: Zelphati, O., & Szoka, F. C.
Affiliations: University of California, San Francisco (US)
ORCID: Zelphati, O
Szoka, F C
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1996-10-15
Volume: 93
Issue: 21
Pages: 11493-11498
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.93.21.11493
PMC: PMC38085
OpenAlex: W2018116983
Cited by: 634
References: 22
Citations by year: 2026: 10 · 2025: 12 · 2024: 14 · 2023: 9 · 2022: 9 · 2021: 16 · 2020: 15 · 2019: 26 · 2018: 19 · 2017: 17 · 2016: 21 · 2015: 14 · 2014: 20 · 2013: 24 · 2012: 27
FWCI: 8.46
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r01dk046052-07, 5r01gm030163-11)
National Institute of General Medical Sciences (GM30163)
National Institute of Diabetes and Digestive and Kidney Diseases (DK-46052)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
Keywords: cationic liposomes, anionic liposomes, phosphatidylserine, lipid flip-flop, endocytosis, nuclear delivery, charge ratio, ion pairing, lipid lateral diffusion, dextran sulfate, heparin, nucleic acid delivery
MeSH terms: Base Sequence, Cell Membrane, DNA, Endocytosis, Energy Transfer, Hydrogen-Ion Concentration, Liposomes, Models, Biological, Oligodeoxyribonucleotides, Osmolar Concentration, RNA, Transfer, Serum Albumin, Bovine, Spectrometry, Fluorescence, Spermidine, Spermine, Structure-Activity Relationship, Thionucleotides, Microscopy, Confocal

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Mechanism of oligonucleotide release from cationic liposomes.

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