The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Calcium-dependent switching of the specificity of phosphoinositide binding to synaptotagmin
Open accessAbstract
The synaptic vesicle membrane protein synaptotagmin (tagmin) is essential for fast, calcium-dependent, neurotransmitter release and is likely to be the calcium sensor for exocytosis, because of its many calcium-dependent properties. Polyphosphoinositides are needed for exocytosis, but it has not been known why. We now provide a possible connection between these observations with the finding that the C2B domain of tagmin I binds phosphatidylinositol-4,5-bisphosphate (PIns-4,5-P 2 ), its isomer phosphatidylinositol-3,4-bisphosphate and phosphatidylinositol-3,4,5-trisphosphate (PIns-3,4,5-P 3 ). Calcium ions switch the specificity of this binding from PIns-3,4,5-P 3 (at calcium concentrations found in resting nerve terminals) to PIns-4,5-P 2 (at concentration of calcium required for transmitter release). Inositol polyphosphates, known blockers of neurotransmitter release, inhibit the binding of both PIns-4,5-P 2 and PIns-3,4,5-P 3 to tagmin. Our findings imply that tagmin may operate as a bimodal calcium sensor, switching bound lipids during exocytosis. This connection to polyphosphoinositides, compounds whose levels are physiologically regulated, could be important for long-term memory and learning.
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Cites
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Cited by
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Metadata
- Authors
- Schiavo, G., Gu, Q.-M., Prestwich, G. D., Söllner, T. H., & Rothman, J. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-11-12
- Volume
- 93
- Issue
- 23
- Pages
- 13327-13332
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1982630258
- Cited by
- 297
- References
- 42
- FWCI
- 7.45
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Calcium-dependent switching of the specificity of phosphoinositide binding to synaptotagmin. https://app.cochat.ai/papers/10.1073/pnas.93.23.13327
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- Schiavo, G., Gu, Q.-M., Prestwich, G. D., Söllner, T. H., & Rothman, J. E. (1996). Calcium-dependent switching of the specificity of phosphoinositide binding to synaptotagmin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.23.13327
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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