Syntaxin: A Synaptic Protein Implicated in Docking of Synaptic Vesicles at Presynaptic Active Zones
Abstract
Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Two 35-kilodalton proteins (p35 or syntaxins) were identified that interact with the synaptic vesicle protein p65 (synaptotagmin). The p35 proteins are expressed only in the nervous system, are 84 percent identical, include carboxyl-terminal membrane anchors, and are concentrated on the plasma membrane at synaptic sites. An antibody to p35 immunoprecipitated solubilized N-type calcium channels. The p35 proteins may function in docking synaptic vesicles near calcium channels at presynaptic active zones.
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Syt1 interacts with the neuronal SNARE complex.
“Syt1 also interacts with the neuronal SNARE complex based on immunoprecipitation and pull-downs24–27, single molecule fluorescence resonance energy transfer (smFRET)28, and nuclear magnetic resonance29,30 experiments.”
From Architecture of the synaptotagmin–SNARE machinery for neuronal exocytosis (2015) · cites this paper as [24]
Proteins regulated by chr3q28 region included five members of the syntaxin family and five ephrin family members.
“Proteins regulated by this region included five members of the syntaxin family involved in synapse function31 and five ephrin family members involved in neural development and memory32,33.”
From Proteogenomic analysis of human cerebrospinal fluid identifies neurologically relevant regulation and implicates causal proteins for Alzheimer’s disease (2024) · cites this paper as [31]
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- Authors
- Bennett, M. K., Calakos, N., & Scheller, R. H.
- Affiliations
- Howard Hughes Medical Institute (US)
- Venue
- Science
- Published
- 1992-07-10
- Volume
- 257
- Issue
- 5067
- Pages
- 255-259
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2056293200
- Cited by
- 1,263
- References
- 1
- Citations by year
- 2026: 8 · 2025: 9 · 2024: 10 · 2023: 10 · 2022: 17 · 2021: 23 · 2020: 11 · 2019: 13 · 2018: 15 · 2017: 26 · 2016: 23 · 2015: 26 · 2014: 35 · 2013: 29 · 2012: 23
- FWCI
- 39.02
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service (2T32G07365)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- syntaxin, synaptic vesicle docking, active zone, calcium-regulated exocytosis, neurotransmitter release, synaptotagmin, p35, N-type calcium channels, synaptic proteins
- MeSH terms
- Amino Acid Sequence, Animals, Antigens, Surface, Calcium-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Membrane Glycoproteins, Molecular Sequence Data, Nerve Tissue Proteins, Synaptic Transmission, Sequence Homology, Nucleic Acid, Synaptic Vesicles, Immunoblotting, Oligonucleotide Probes, Syntaxin 1, Synaptotagmins, Synaptotagmin I, Rats
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