The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Role of guanine nucleotide-binding proteins--ras-family or trimeric proteins or both--in Ca2+ sensitization of smooth muscle.
Open accessAbstract
The purpose of this study was to identify guanine nucleotide-binding proteins (G proteins) involved in the agonist- and guanosine 5'-[gamma-thio]triphosphate (GTP[gamma-S])-induced increase in the Ca2+ sensitivity of 20-kDa myosin light chain (MLC20) phosphorylation and contraction in smooth muscle. A constitutively active, recombinant val14p21rhoA.GTP expressed in the baculovirus/Sf9 system, but not the protein expressed without posttranslational modification in Escherichia coli, induced at constant Ca2+ (pCa 6.4) a slow contraction associated with increased MLC20 phosphorylation from 19.8% to 29.5% (P < 0.05) in smooth muscle permeabilized with beta-esein. The effect of val14p21rhoA.GTP was inhibited by ADP-ribosylation of the protein and was absent in smooth muscle extensively permeabilized with Triton X-100. ADP-ribosylation of endogenous p21rho with epidermal cell differentiation inhibitor (EDIN) inhibited Ca2+ sensitization induced by GTP [in rabbit mesenteric artery (RMA) and rabbit ileum smooth muscles], by carbachol (in rabbit ileum), and by endothelin (in RMA), but not by phenylephrine (in RMA), and only slowed the rate without reducing the amplitude of contractions induced in RMA by 1 microM GTP[gamma-S] at constant Ca2+ concentrations. AlF(4-)-induced Ca2+ sensitization was inhibited by both guanosine 5'-[beta-thio]diphosphate (GDP[beta-S]) and by EDIN. EDIN also inhibited, to a lesser extent, contractions induced by Ca2+ alone (pCa 6.4) in both RMA and rabbit ileum. ADP-ribosylation of trimeric G proteins with pertussis toxin did not inhibit Ca2+ sensitization. We conclude that p21rho may play a role in physiological Ca2+ sensitization as a cofactor with other messengers, rather than as a sole direct inhibitor of smooth muscle MLC20 phosphatase.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Calculation of protein extinction coefficients from amino acid sequence data
Gill, S. C., & von Hippel, P. H. (1989) · Analytical Biochemistry · doi:10.1016/0003-2697(89)90602-7 · Cited by 5,283
Ridley, A. J., & Hall, A. (1992) · Cell · doi:10.1016/0092-8674(92)90163-7 · Cited by 4,468
Self-citationSignal transduction and regulation in smooth muscle
Somlyo, A. P., & Somlyo, A. V. (1994) · Nature · doi:10.1038/372231a0 · Cited by 1,902
Self-citationPhysiology of the Gastrointestinal Tract.
(1987) · Annals of Internal Medicine · doi:10.7326/0003-4819-107-1-131_1 · Cited by 1,673
Kurose, H., Katada, T., Amano, T., & Ui, M. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32507-9 · Cited by 494
Involvement of rho p21 in the GTP-enhanced calcium ion sensitivity of smooth muscle contraction.
Hirata, K., Kikuchi, A., Sasaki, T., Kuroda, S., Kaibuchi, K., Matsuura, Y., Seki, H., Saida, K., & Takai, Y. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)50337-4 · Cited by 447
Receptor-coupled, Permeabilized Smooth Muscle
Kitazawa, T., Kobayashi, S., Horiuti, K., Somlyo, A. V., & Somlyo, A. P. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)83550-5 · Cited by 427
Self-citationALESSI, D., MACDOUGALL, L. K., SOLA, M. M., IKEBE, M., & COHEN, P. (1992) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1992.tb17508.x · Cited by 377
Cytosolic heparin inhibits muscarinic and α-adrenergic Ca2+ release in smooth muscle
Kobayashi, S., Kitazawa, T., Somlyo, A. V., & Somlyo, A. P. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)84670-7 · Cited by 337
Self-citationG protein-mediated inhibition of myosin light-chain phosphatase in vascular smooth muscle.
Kitazawa, T., Masuo, M., & Somlyo, A. P. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.20.9307 · Cited by 320
Self-citation
Cited by
Myofibroblasts and mechano-regulation of connective tissue remodelling
Tomasek, J. J., Gabbiani, G., Hinz, B., Chaponnier, C., & Brown, R. A. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm809 · Cited by 4,290
Somlyo, A. P., & Somlyo, A. V. (2000) · The Journal of Physiology · doi:10.1111/j.1469-7793.2000.t01-2-00177.x · Cited by 1,207
Self-citationFormation of actin stress fibers and focal adhesions enhanced by Rho-kinase
Amano, M., Chihara, K., Kimura, K., Fukata, Y., Nakamura, N., Matsuura, Y., & Kaibuchi, K. (1997) · Science · doi:10.1126/science.275.5304.1308 · Cited by 1,077
Regulation of the Cytoskeleton and Cell Adhesion by the Rho Family GTPases in Mammalian Cells
Kaibuchi, K., Kuroda, S., & Amano, M. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.459 · Cited by 1,017
Sauzeau, V., Le Jeune, H., Cario-Toumaniantz, C., Smolenski, A., Lohmann, S. M., Bertoglio, J., Chardin, P., Pacaud, P., & Loirand, G. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m000753200 · Cited by 602
Metadata
- Authors
- Gong, M. C., Iizuka, K., Nixon, G., Browne, J. P., Hall, A., Eccleston, J. F., Sugai, M., Kobayashi, S., Somlyo, A. V., & Somlyo, A. P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-02-06
- Volume
- 93
- Issue
- 3
- Pages
- 1340-1345
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2062129029
- Cited by
- 277
- References
- 29
- FWCI
- 10.02
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Role of guanine nucleotide-binding proteins--ras-family or trimeric proteins or both--in Ca2+ sensitization of smooth muscle. https://app.cochat.ai/papers/10.1073/pnas.93.3.1340
- The paper
- Gong, M. C., Iizuka, K., Nixon, G., Browne, J. P., Hall, A., Eccleston, J. F., Sugai, M., Kobayashi, S., Somlyo, A. V., & Somlyo, A. P. (1996). Role of guanine nucleotide-binding proteins--ras-family or trimeric proteins or both--in Ca2+ sensitization of smooth muscle. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.3.1340
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
