The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
h CTR1: A human gene for copper uptake identified by complementation in yeast
Open accessAbstract
The molecular mechanisms responsible for the cellular uptake of copper in mammalian cells are unknown. We describe isolation of a human gene involved in this process by complementation of the yeast high-affinity copper uptake mutant,ctr1. Besides complementing ctr1 growth defect on nonfermentable media, the human gene also rescues iron transport and SOD1 defects in ctr1 yeast. Overexpression of the gene in yeast leads to vulnerability to the toxicity of copper overload. In addition, its expression in ctr1 yeast significantly increases the level of cellular copper, as demonstrated by atomic absorption. We propose this gene as a candidate for high-affinity copper uptake in humans and by analogy have named it h CTR1. The hCTR1 and yeast CTR1 predicted transmembrane proteins are 29% identical, but the human protein is substantially smaller in both the extracellular metal-binding and intracellular domains. An additional human gene similar to h CTR1, here named h CTR2, was identified in a database search. Both h CTR1 and h CTR2 are expressed in all human tissues examined, and both genes are located in 9q31/32. These studies, together with the previously recognized functional and sequence similarity between the Menkes/Wilson copper export proteins and CCC2 in yeast, demonstrate that similar copper homeostatic mechanisms are used in these evolutionarily divergent organisms.
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The Wilson disease gene is a putative copper transporting P–type ATPase similar to the Menkes gene
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Self-citationThe Wilson disease gene is a copper transporting ATPase with homology to the Menkes disease gene
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Metadata
- Authors
- Zhou, B., & Gitschier, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-07-08
- Volume
- 94
- Issue
- 14
- Pages
- 7481-7486
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2010355719
- Cited by
- 567
- References
- 35
- FWCI
- 21.93
- Type
- journal article
- Language
- en
- Open access
- green
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