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Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation
Open accessAbstract
The yeast translation factor eIF4G associates with both the cap-binding protein eIF4E and the poly(A)-binding protein Pab1p. Here we report that the two yeast eIF4G homologs, Tif4631p and Tif4632p, share a conserved Pab1p-binding site. This site is required for Pab1p and poly(A) tails to stimulate the in vitro translation of uncapped polyadenylylated mRNA, and the region encompassing it is required for the cap and the poly(A) tail to synergistically stimulate translation. This region on Tif4631p becomes essential for cell growth when the eIF4E binding site on Tif4631p is mutated. Pab1p mutations also show synthetic lethal interactions with eIF4E mutations. These data suggest that eIF4G mediates poly(A) tail stimulated translation in vitro , and that Pab1p and the domain encompassing the Pab1p-binding site on eIF4G can compensate for partial loss of eIF4E function in vivo .
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Cited by
Regulation of mRNA Translation and Stability by microRNAs
Fabian, M. R., Sonenberg, N., & Filipowicz, W. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060308-103103 · Cited by 3,214
eIF4 Initiation Factors: Effectors of mRNA Recruitment to Ribosomes and Regulators of Translation
Gingras, A.-C., Raught, B., & Sonenberg, N. (1999) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.68.1.913 · Cited by 2,121
The Scanning Mechanism of Eukaryotic Translation Initiation
Hinnebusch, A. G. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035802 · Cited by 798
Imataka, H., Gradi, A., & Sonenberg, N. (1998) · The EMBO Journal · doi:10.1093/emboj/17.24.7480 · Cited by 572
The Mechanism of Eukaryotic Translation Initiation: New Insights and Challenges
Hinnebusch, A. G., & Lorsch, J. R. (2012) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a011544 · Cited by 503
Metadata
- Authors
- Tarun, S. Z., Wells, S. E., Deardorff, J. A., & Sachs, A. B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-08-19
- Volume
- 94
- Issue
- 17
- Pages
- 9046-9051
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1973433187
- Cited by
- 316
- References
- 0
- FWCI
- 9.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Tarun, S. Z., Wells, S. E., Deardorff, J. A., & Sachs, A. B. (1997). Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.17.9046
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