The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Substitution of a mutant α 2a -adrenergic receptor via “hit and run” gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo
Open accessAbstract
Norepinephrine contributes to antinociceptive, sedative, and sympatholytic responses in vivo , and α 2 adrenergic receptor (α 2 AR) agonists are used clinically to mimic these effects. Lack of subtype-specific agonists has prevented elucidation of the role that each α 2 AR subtype (α 2A , α 2B , and α 2C ) plays in these central effects. Here we demonstrate that α 2 AR agonist-elicited sedative, anesthetic-sparing, and analgesic responses are lost in a mouse line expressing a subtly mutated α 2A AR, D79N α 2A AR, created by two-step homologous recombination. These functional changes are accompanied by failure of the D79N α 2A AR to inhibit voltage-gated Ca 2+ currents and spontaneous neuronal firing, a measure of K + current activation. These results provide definitive evidence that the α 2A AR subtype is the primary mediator of clinically important central actions of α 2 AR agonists and suggest that the D79N α 2A AR mouse may serve as a model for exploring other possible α 2A AR functions in vivo .
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Cites
International Union of Pharmacology nomenclature of adrenoceptors.
Bylund, D. B., Eikenberg, D. C., Hieble, J. P., Langer, S. Z., Lefkowitz, R. J., Minneman, K. P., Molinoff, P. B., Ruffolo, R. R., & Trendelenburg, U. (1994) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06780-8 · Cited by 1,218
Sequence Alignment of the G-Protein Coupled Receptor Superfamily
PROBST, W. C., SNYDER, L. A., SCHUSTER, D. I., BROSIUS, J., & SEALFON, S. C. (1992) · DNA and Cell Biology · doi:10.1089/dna.1992.11.1 · Cited by 845
The Pharmacological Basis of Therapeutics.
Goodman, L., & Gilman, A. (1941) · Anesthesia & Analgesia · doi:10.1213/00000539-194101000-00064 · Cited by 599
Pharmacology of spinal adrenergic systems which modulate spinal nociceptive processing
Yaksh, T. L. (1985) · Pharmacology Biochemistry and Behavior · doi:10.1016/0091-3057(85)90537-4 · Cited by 586
The locus coeruleus: A cytoarchitectonic, golgi and immunohistochemical study in the albino rat
Swanson, L. W. (1976) · Brain Research · doi:10.1016/0006-8993(76)90207-9 · Cited by 518
Central Hypotensive Effects of the α 2a -Adrenergic Receptor Subtype
MacMillan, L. B., Hein, L., Smith, M. S., Piascik, M. T., & Limbird, L. E. (1996) · Science · doi:10.1126/science.273.5276.801 · Cited by 489
Self-citationAghajanian, G. K., & VanderMaelen, C. P. (1982) · Science · doi:10.1126/science.6278591 · Cited by 431
A Hypnotic Response to Dexmedetomidine, an α2 Agonist, Is Mediated in the Locus Coerüleus in Rats
Correa-Sales, C., Rabin, B. C., & Maze, M. (1992) · Anesthesiology · doi:10.1097/00000542-199206000-00013 · Cited by 394
Self-citationReceptors linked to inhibition of adenylate cyclase: additional signaling mechanisms
Limbird, L. E. (1988) · The FASEB Journal · doi:10.1096/fasebj.2.11.2840317 · Cited by 342
Self-citationIntroduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells
Hasty, P., Ramírez-Solis, R., Krumlauf, R., & Bradley, A. (1991) · Nature · doi:10.1038/350243a0 · Cited by 331
Cited by
Millan, M. J. (2002) · Progress in Neurobiology · doi:10.1016/s0301-0082(02)00009-6 · Cited by 3,035
Molecular and neuronal substrates for general anaesthetics
Rudolph, U., & Antkowiak, B. (2004) · Nature Reviews Neuroscience · doi:10.1038/nrn1496 · Cited by 772
General anaesthetic actions on ligand-gated ion channels
Krasowski, M. D., & Harrison, N. L. (1999) · Cellular and Molecular Life Sciences (CMLS) · doi:10.1007/s000180050371 · Cited by 384
Grandjean, J., Schroeter, A., Batata, I., & Rudin, M. (2014) · NeuroImage · doi:10.1016/j.neuroimage.2014.08.043 · Cited by 339
Physiological significance of α2-adrenergic receptor subtype diversity: one receptor is not enough
Philipp, M., Brede, M., & Hein, L. (2002) · American Journal of Physiology-Regulatory, Integrative and Comparative Physiology · doi:10.1152/ajpregu.00123.2002 · Cited by 318
Metadata
- Authors
- Lakhlani, P. P., MacMillan, L. B., Guo, T. Z., McCool, B. A., Lovinger, D. M., Maze, M., & Limbird, L. E.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-09-02
- Volume
- 94
- Issue
- 18
- Pages
- 9950-9955
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1988468898
- Cited by
- 360
- References
- 39
- FWCI
- 6.64
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Substitution of a mutant α 2a -adrenergic receptor via “hit and run” gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo. https://app.cochat.ai/papers/10.1073/pnas.94.18.9950
- The paper
- Lakhlani, P. P., MacMillan, L. B., Guo, T. Z., McCool, B. A., Lovinger, D. M., Maze, M., & Limbird, L. E. (1997). Substitution of a mutant α 2a -adrenergic receptor via “hit and run” gene targeting reveals the role of this subtype in sedative, analgesic, and anesthetic-sparing responses in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.18.9950
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