The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector
Open accessAbstract
The development of methods for efficient gene transfer to terminally differentiated retinal cells is important to study the function of the retina as well as for gene therapy of retinal diseases. We have developed a lentiviral vector system based on the HIV that can transduce terminally differentiated neurons of the brain in vivo . In this study, we have evaluated the ability of HIV vectors to transfer genes into retinal cells. An HIV vector containing a gene encoding the green fluorescent protein (GFP) was injected into the subretinal space of rat eyes. The GFP gene under the control of the cytomegalovirus promoter was efficiently expressed in both photoreceptor cells and retinal pigment epithelium. However, the use of the rhodopsin promoter resulted in expression predominantly in photoreceptor cells. Most successfully transduced eyes showed that photoreceptor cells in >80% of the area of whole retina expressed the GFP. The GFP expression persisted for at least 12 weeks with no apparent decrease. The efficient gene transfer into photoreceptor cells by HIV vectors will be useful for gene therapy of retinal diseases such as retinitis pigmentosa.
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Cites
In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector
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Self-citationA common progenitor for neurons and glia persists in rat retina late in development
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Self-citationHighly efficient and sustained gene transfer in adult neurons with a lentivirus vector
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Self-citationA "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells
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Cited by
A Third-Generation Lentivirus Vector with a Conditional Packaging System
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Gene therapy - promises, problems and prospects
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Self-citationCas9 as a versatile tool for engineering biology
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Development of a self-inactivating lentivirus vector
Miyoshi, H., Blömer, U., Takahashi, M., Gage, F. H., & Verma, I. M. (1998) · Journal of Virology · doi:10.1128/jvi.72.10.8150-8157.1998 · Cited by 1,196
Self-citationWnt-mediated activation of NeuroD1 and retro-elements during adult neurogenesis
Kuwabara, T., Hsieh, J., Muotri, A., Yeo, G., Warashina, M., Lie, D. C., Moore, L., Nakashima, K., Asashima, M., & Gage, F. H. (2009) · Nature Neuroscience · doi:10.1038/nn.2360 · Cited by 648
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Metadata
- Authors
- Miyoshi, H., Takahashi, M., Gage, F. H., & Verma, I. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-09-16
- Volume
- 94
- Issue
- 19
- Pages
- 10319-10323
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2008512248
- Cited by
- 494
- References
- 28
- FWCI
- 18.07
- Type
- journal article
- Language
- en
- Open access
- green
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