Cas9 as a versatile tool for engineering biology

A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity

Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012) · Science · doi:10.1126/science.1225829 · Cited by 17,116 · see citing works

Multiplex Genome Engineering Using CRISPR/Cas Systems

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RNA-Guided Human Genome Engineering via Cas9

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Circular RNAs are a large class of animal RNAs with regulatory potency

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Natural RNA circles function as efficient microRNA sponges

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Folding DNA to create nanoscale shapes and patterns

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CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

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Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

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DNA targeting specificity of RNA-guided Cas9 nucleases

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A System for Stable Expression of Short Interfering RNAs in Mammalian Cells

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CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

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One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

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High-Frequency off-Target Mutagenesis Induced by CRISPR-Cas Nucleases in Human Cells

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Efficient genome editing in zebrafish using a CRISPR-Cas system

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Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

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Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

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CRISPR RNA Maturation by Trans-Encoded Small RNA and Host Factor RNase III

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Evolution and classification of the CRISPR–Cas systems

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CRISPR/Cas, the Immune System of Bacteria and Archaea

Horvath, P., & Barrangou, R. (2010) · Science · doi:10.1126/science.1179555 · Cited by 2,471 · see citing works

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

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Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena

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Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum

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Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.

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RNA-programmed genome editing in human cells

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MicroRNA biogenesis: coordinated cropping and dicing

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Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system

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Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

Cho, S. W., Kim, S., Kim, J. M., & Kim, J.-S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2507 · Cited by 1,863 · see citing works

RNA-guided genetic silencing systems in bacteria and archaea

Wiedenheft, B., Sternberg, S. H., & Doudna, J. A. (2012) · Nature · doi:10.1038/nature10886 · Cited by 1,851 · see citing works

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

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Gene therapy - promises, problems and prospects

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Highly efficient endogenous human gene correction using designed zinc-finger nucleases

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Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

DiCarlo, J. E., Norville, J. E., Mali, P., Rios, X., Aach, J., & Church, G. M. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt135 · Cited by 1,566 · see citing works

Reshaping human antibodies for therapy

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Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

Li, J., Norville, J. E., Aach, J., McCormack, M., Zhang, D., Bush, J., Church, G. M., & Sheen, J. (2013) · Nature Biotechnology · doi:10.1038/nbt.2654 · Cited by 1,499 · see citing works

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

Pattanayak, V., Lin, S., Guilinger, J. P., Ma, E., Doudna, J. A., & Liu, D. R. (2013) · Nature Biotechnology · doi:10.1038/nbt.2673 · Cited by 1,487 · see citing works

RNA-guided gene activation by CRISPR-Cas9-based transcription factors

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CRISPR RNA–guided activation of endogenous human genes

Maeder, M. L., Linder, S. J., Cascio, V. M., Fu, Y., Ho, Q. H., & Joung, J. K. (2013) · Nature Methods · doi:10.1038/nmeth.2598 · Cited by 1,249 · see citing works

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

Nekrasov, V., Staskawicz, B. J., Weigel, D., Jones, J. D. G., & Kamoun, S. (2013) · Nature Biotechnology · doi:10.1038/nbt.2655 · Cited by 1,123 · see citing works

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

Bikard, D., Jiang, W., Samai, P., Hochschild, A., Zhang, F., & Marraffini, L. A. (2013) · Nucleic Acids Research · doi:10.1093/nar/gkt520 · Cited by 1,111 · see citing works

Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

Gratz, S. J., Cummings, A. M., Nguyen, J. N., Hamm, D. C., Donohue, L. K., Harrison, M. M., Wildonger, J., & O’Connor-Giles, K. M. (2013) · Genetics · doi:10.1534/genetics.113.152710 · Cited by 959 · see citing works

CRISPR-Cas Systems in Bacteria and Archaea: Versatile Small RNAs for Adaptive Defense and Regulation

Bhaya, D., Davison, M., & Barrangou, R. (2011) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132430 · Cited by 914 · see citing works

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

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Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery

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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

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Dynamic genome architecture in the nuclear space: regulation of gene expression in three dimensions

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Diversity, Activity, and Evolution of CRISPR Loci in Streptococcus thermophilus

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Efficient construction of sequence-specific TAL effectors for modulating mammalian transcription

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Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor

Deng, W., Lee, J., Wang, H., Miller, J., Reik, A., Gregory, P. D., Dean, A., & Blobel, G. A. (2012) · Cell · doi:10.1016/j.cell.2012.03.051 · Cited by 734 · see citing works

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

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A system for the continuous directed evolution of biomolecules

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Self versus non-self discrimination during CRISPR RNA-directed immunity

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Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

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Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells

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In vivo genome editing restores haemostasis in a mouse model of haemophilia

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Phage Integrases: Biology and Applications

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Enhanced Efficiency of Human Pluripotent Stem Cell Genome Editing through Replacing TALENs with CRISPRs

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Stable and efficient gene transfer into the retina using an HIV-based lentiviral vector

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RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

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CRISPR-based adaptive immune systems

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Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

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Positive and negative regulation of endogenous genes by designed transcription factors

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Cellular Self-Defense: How Cell-Autonomous Immunity Protects Against Pathogens

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Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs

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Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis

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Scalable gene synthesis by selective amplification of DNA pools from high-fidelity microchips

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Synergistic and tunable human gene activation by combinations of synthetic transcription factors

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Robust, synergistic regulation of human gene expression using TALE activators

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Advances in adenoviral vectors: from genetic engineering to their biology

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Engineered zinc finger nickases induce homology-directed repair with reduced mutagenic effects

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Precision genome engineering with programmable DNA-nicking enzymesPreprint

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Adeno-associated Virus as a Gene Therapy Vector: Vector Development, Production and Clinical Applications

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Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzymePreprint

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A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells

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Monomeric site-specific nucleases for genome editing

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Barcoding cells using cell-surface programmable DNA-binding domains

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The new frontier of genome engineering with CRISPR-Cas9

Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062 · see citing works

Easy quantitative assessment of genome editing by sequence trace decomposition

Brinkman, E. K., Chen, T., Amendola, M., & van Steensel, B. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku936 · Cited by 2,390 · see citing works

An updated evolutionary classification of CRISPR–Cas systems

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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

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Highly efficient Cas9-mediated transcriptional programming

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Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

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Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

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Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

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Mechanisms and Regulation of Alternative Pre-mRNA Splicing

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Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

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Structures of Cas9 endonucleases reveal RNA-mediated conformational activation

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Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

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CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

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CRISPR/Cas9 in Genome Editing and Beyond

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