The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability
Open accessAbstract
Elucidation of mechanisms that regulate hematopoietic stem cell self-renewal and differentiation would be facilitated by the identification of defined culture conditions that allow these cells to be amplified. We now demonstrate a significant net increase (3-fold,P< 0.001)in vitro of cells that are individually able to permanently and competitively reconstitute the lymphoid and myeloid systems of syngeneic recipient mice when Sca-1+lin−adult marrow cells are incubated for 10 days in serum-free medium with interleukin 11, flt3-ligand, and Steel factor. Moreover, the culture-derived repopulating cells continued to expand their numbers in the primary hosts at the same rate seen in recipients of noncultured stem cells. In the expansion cultures, long-term culture-initiating cells increased 7- ± 2-fold, myeloid colony-forming cells increased 140- ± 36-fold, and total nucleated cells increased 230- ± 62-fold. Twenty-seven of 100 cultures initiated with 15 Sca-1+lin−marrow cells were found to contain transplantable stem cells 10 days later. This frequency of positive cultures is the same as the frequency of transplantable stem cells in the original input suspension, suggesting that most had undergone at least one self-renewal division in vitro. No expansion of stem cells was seen when Sca-1+TER119−CD34+day 14.5 fetal liver cells were cultured under the same conditions. These findings set the stage for further investigations of the mechanisms by which cytokine stimulation may elicit different outcomes in mitotically activated hematopoietic stem cells during ontogeny and in the adult.
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Cites
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Self-citationThe purification and characterization of fetal liver hematopoietic stem cells
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Cited by
Stem cells, cancer, and cancer stem cells
Reya, T., Morrison, S. J., Clarke, M. F., & Weissman, I. L. (2001) · Nature · doi:10.1038/35102167 · Cited by 9,738
A role for Wnt signalling in self-renewal of haematopoietic stem cells
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Long-Term Propagation of Distinct Hematopoietic Differentiation Programs In Vivo
Dykstra, B., Kent, D., Bowie, M., McCaffrey, L., Hamilton, M., Lyons, K., Lee, S.-J., Brinkman, R., & Eaves, C. (2007) · Cell Stem Cell · doi:10.1016/j.stem.2007.05.015 · Cited by 609
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Metadata
- Authors
- Miller, C. L., & Eaves, C. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-12-09
- Volume
- 94
- Issue
- 25
- Pages
- 13648-13653
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2088255007
- Cited by
- 261
- References
- 38
- FWCI
- 8.89
- Type
- journal article
- Language
- en
- Open access
- green
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- Miller, C. L., & Eaves, C. J. (1997). Expansion in vitro of adult murine hematopoietic stem cells with transplantable lympho-myeloid reconstituting ability. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.25.13648
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