The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry
Open accessAbstract
Nano-electrospray tandem mass spectrometry allows qualitative and quantitative analysis of complex membrane lipid mixtures at the subpicomole level. We have exploited this technique to selectively detect individual classes of phospholipids from unprocessed total cellular lipid extracts by either precursor ion or neutral loss scanning. This way phosphatidylcholine, sphingomyelin, phosphatidylinositol and -phosphates, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidic acid, and their plasmalogen analogues can be detected. The optimized ionization and fragmentation conditions described together with the principle of internal standardization by nonnatural analogues allow the rapid and quantitative determination of membrane lipid compositions down to sample amounts of 1000 cells.
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
Bligh, E. G., & Dyer, W. J. (1959) · Canadian Journal of Biochemistry and Physiology · doi:10.1139/o59-099 · Cited by 54,751
Functional rafts in cell membranes
Simons, K., & Ikonen, E. (1997) · Nature · doi:10.1038/42408 · Cited by 9,757
Electrospray Ionization for Mass Spectrometry of Large Biomolecules
Fenn, J. B., Mann, M., Meng, C. K., Wong, S. F., & Whitehouse, C. M. (1989) · Science · doi:10.1126/science.2675315 · Cited by 7,634
Cited by
Molecular anatomy of a trafficking organelle
Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., Urlaub, H., Schenck, S., Brügger, B., Ringler, P., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.030 · Cited by 2,373
Self-citationHan, X., & Gross, R. W. (2003) · Journal of Lipid Research · doi:10.1194/jlr.r300004-jlr200 · Cited by 914
Schneiter, R., Brügger, B., Sandhoff, R., Zellnig, G., Leber, A., Lampl, M., Athenstaedt, K., Hrastnik, C., Eder, S., Daum, G., et al. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.146.4.741 · Cited by 493
Self-citationKoivusalo, M., Haimi, P., Heikinheimo, L., Kostiainen, R., & Somerharju, P. (2001) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)31176-7 · Cited by 417
Shaner, R. L., Allegood, J. C., Park, H., Wang, E., Kelly, S., Haynes, C. A., Sullards, M. C., & Merrill, A. H. (2009) · Journal of Lipid Research · doi:10.1194/jlr.d800051-jlr200 · Cited by 399
Metadata
- Authors
- Brügger, B., Erben, G., Sandhoff, R., Wieland, F. T., & Lehmann, W. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1997-03-18
- Volume
- 94
- Issue
- 6
- Pages
- 2339-2344
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2077727777
- Cited by
- 881
- References
- 3
- FWCI
- 6.49
- Type
- journal article
- Language
- en
- Open access
- green
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- Brügger, B., Erben, G., Sandhoff, R., Wieland, F. T., & Lehmann, W. D. (1997). Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.94.6.2339
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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