The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
Open accessAbstract
A split intein capable of protein trans -splicing is identified in a DnaE protein of the cyanobacterium Synechocystis sp. strain PCC6803. The N- and C-terminal halves of DnaE (catalytic subunit α of DNA polymerase III) are encoded by two separate genes, dnaE-n and dnaE-c , respectively. These two genes are located 745,226 bp apart in the genome and on opposite DNA strands. The dnaE-n product consists of a N-extein sequence followed by a 123-aa intein sequence, whereas the dnaE-c product consists of a 36-aa intein sequence followed by a C-extein sequence. The N- and C-extein sequences together reconstitute a complete DnaE sequence that is interrupted by the intein sequences inside the β- and τ-binding domains. The two intein sequences together reconstitute a split mini-intein that not only has intein-like sequence features but also exhibited protein trans -splicing activity when tested in Escherichia coli cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Basic local alignment search tool
Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990) · Journal of Molecular Biology · doi:10.1006/jmbi.1990.9999 · Cited by 98,669
Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994) · Nucleic Acids Research · doi:10.1093/nar/22.22.4673 · Cited by 64,617
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.185 · Cited by 2,432
Kaneko, T., Sato, S., Kotani, H., Tanaka, A., Asamizu, E., Nakamura, Y., Miyajima, N., Hirosawa, M., Sugiura, M., Sasamoto, S., et al. (1996) · DNA Research · doi:10.1093/dnares/3.3.109 · Cited by 2,132
INTRONS AS MOBILE GENETIC ELEMENTS
Lambowitz, A. M., & Belfort, M. (1993) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.62.070193.003103 · Cited by 625
Kane, P. M., Yamashiro, C. T., Wolczyk, D. F., Neff, N., Goebl, M., & Stevens, T. H. (1990) · Science · doi:10.1126/science.2146742 · Cited by 497
Protein Splicing of Inteins and Hedgehog Autoproteolysis: Structure, Function, and Evolution
Perler, F. B. (1998) · Cell · doi:10.1016/s0092-8674(00)80892-2 · Cited by 369
Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae
Gimble, F. S., & Thorner, J. W. (1992) · Nature · doi:10.1038/357301a0 · Cited by 273
Crystal Structure of PI-SceI, a Homing Endonuclease with Protein Splicing Activity
Duan, X., Gimble, F. S., & Quiocho, F. A. (1997) · Cell · doi:10.1016/s0092-8674(00)80237-8 · Cited by 254
Protein Splicing Involving the Saccharomyces cerevisiae VMA Intein
Chong, S., Shao, Y., Paulus, H., Benner, J., Perler, F. B., & Xu, M.-Q. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.36.22159 · Cited by 243
Cited by
Kaneko, T. (2001) · DNA Research · doi:10.1093/dnares/8.5.205 · Cited by 696
Production of cyclic peptides and proteins in vivo
Scott, C. P., Abel-Santos, E., Wall, M., Wahnon, D. C., & Benkovic, S. J. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13638 · Cited by 402
Nature's recipe for splitting inteins
Aranko, A. S., Wlodawer, A., & Iwai, H. (2014) · Protein Engineering Design and Selection · doi:10.1093/protein/gzu028 · Cited by 116
Can Adeno-Associated Viral Vectors Deliver Effectively Large Genes?
Tornabene, P., & Trapani, I. (2020) · Human Gene Therapy · doi:10.1089/hum.2019.220 · Cited by 76
Tornabene, P., Trapani, I., Centrulo, M., Marrocco, E., Minopoli, R., Lupo, M., Iodice, C., Gesualdo, C., Simonelli, F., Surace, E. M., et al. (2021) · Molecular Therapy - Methods & Clinical Development · doi:10.1016/j.omtm.2021.10.004 · Cited by 25
Metadata
- Authors
- Wu, H., Hu, Z., & Liu, X.-Q.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-08-04
- Volume
- 95
- Issue
- 16
- Pages
- 9226-9231
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2130511335
- Cited by
- 373
- References
- 27
- FWCI
- 4.16
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. https://app.cochat.ai/papers/10.1073/pnas.95.16.9226
- The paper
- Wu, H., Hu, Z., & Liu, X.-Q. (1998). Protein trans -splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.16.9226
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
