The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
c-Myc target gene specificity is determined by a post-DNAbinding mechanism
Open accessAbstract
Uncertainty as to which member of a family of DNA-binding transcription factors regulates a specific promoter in intact cells is a problem common to many investigators. Determining target gene specificity requires both an analysis of protein binding to the endogenous promoter as well as a characterization of the functional consequences of transcription factor binding. By using a formaldehyde crosslinking procedure and Gal4 fusion proteins, we have analyzed the timing and functional consequences of binding of Myc and upstream stimulatory factor (USF)1 to endogenous cellular genes. We demonstrate that the endogenous cad promoter can be immunoprecipitated with antibodies against Myc and USF1. We further demonstrate that although both Myc and USF1 can bind to cad, the cad promoter can respond only to the Myc transactivation domain. We also show that the amount of Myc bound to the cad promoter fluctuates in a growth-dependent manner. Thus, our data analyzing both DNA binding and promoter activity in intact cells suggest that cad is a Myc target gene. In addition, we show that Myc binding can occur at many sites in vivo but that the position of the binding site determines the functional consequences of this binding. Our data indicate that a post-DNA-binding mechanism determines Myc target gene specificity. Importantly, we have demonstrated the feasibility of analyzing the binding of site-specific transcription factors in vivo to single copy mammalian genes.
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Cites
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The ornithine decarboxylase gene is a transcriptional target of c-Myc.
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Cdc25 cell-cycle phosphatase as a target of c-myc
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Cited by
c-Myc-regulated microRNAs modulate E2F1 expression
O'Donnell, K. A., Wentzel, E. A., Zeller, K. I., Dang, C. V., & Mendell, J. T. (2005) · Nature · doi:10.1038/nature03677 · Cited by 2,347
Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress
Kops, G. J. P. L., Dansen, T. B., Polderman, P. E., Saarloos, I., Wirtz, K. W. A., Coffer, P. J., Huang, T.-T., Bos, J. L., Medema, R. H., & Burgering, B. M. T. (2002) · Nature · doi:10.1038/nature01036 · Cited by 1,580
The Myc/Max/Mad Network and the Transcriptional Control of Cell Behavior
Grandori, C., Cowley, S. M., James, L. P., & Eisenman, R. N. (2000) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.16.1.653 · Cited by 1,333
Eilers, M., & Eisenman, R. N. (2008) · Genes & Development · doi:10.1101/gad.1712408 · Cited by 927
HIF-1α induces cell cycle arrest by functionally counteracting Myc
Koshiji, M., Kageyama, Y., Pete, E. A., Horikawa, I., Barrett, J. C., & Huang, L. E. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600196 · Cited by 652
Metadata
- Authors
- Boyd, K. E., Wells, J., Gutman, J., Bartley, S. M., & Farnham, P. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1998-11-10
- Volume
- 95
- Issue
- 23
- Pages
- 13887-13892
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1974279777
- Cited by
- 276
- References
- 27
- FWCI
- 5.52
- Type
- journal article
- Language
- en
- Open access
- green
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- Boyd, K. E., Wells, J., Gutman, J., Bartley, S. M., & Farnham, P. J. (1998). c-Myc target gene specificity is determined by a post-DNAbinding mechanism. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.95.23.13887
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