Cited by 420DNA Repair MechanismsFungal and yeast genetics researchBacterial Genetics and Biotechnology

Global response of Saccharomyces cerevisiae to an alkylating agent

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Jelinsky, Scott A., Samson, Leona D.·1999·Proceedings of the National Academy of Sciences

Abstract

DNA chip technology enables simultaneous examination of how ≈6,200 Saccharomyces cerevisiae gene transcript levels, representing the entire genome, respond to environmental change. By using chips bearing oligonucleotide arrays, we show that, after exposure to the alkylating agent methyl methanesulfonate, ≈325 gene transcript levels are increased and ≈76 are decreased. Of the 21 genes that already were known to be induced by a DNA-damaging agent, 18 can be scored as inducible in this data set, and surprisingly, most of the newly identified inducible genes are induced even more strongly than these 18. We examined 42 responsive and 8 nonresponsive ORFs by conventional Northern blotting, and 48 of these 50 ORFs responded as they did by DNA chip analysis, with magnitudes displaying a correlation coefficient of 0.79. Responsive genes fall into several expected and many unexpected categories. Evidence for the induction of a program to eliminate and replace alkylated proteins is presented.

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Crossref · 10.1073/pnas.96.4.1486

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Authors
Jelinsky, S. A., & Samson, L. D.
Affiliations
Harvard University (US)
Corresponding author
Samson, Leona D.
Venue
Proceedings of the National Academy of Sciences
Published
1999-02-16
Volume
96
Issue
4
Pages
1486-1491
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2155675863
Cited by
419
References
36
Citations by year
2026: 4 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 1 · 2020: 5 · 2019: 6 · 2018: 6 · 2017: 4 · 2016: 5 · 2015: 11 · 2014: 10 · 2013: 8 · 2012: 11
FWCI
17.46
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p01hg001323-03)
  • National Human Genome Research Institute (P01 HG 01323)
  • National Cancer Institute (CA-55042, CA09078, T32CA009078, R01CA055042)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Saccharomyces cerevisiae, methyl methanesulfonate, alkylating agents, DNA damage response, gene expression profiling, transcriptome, DNA repair genes
MeSH terms
Alkylating Agents, DNA Damage, Fungal Proteins, Genes, Fungal, Methyl Methanesulfonate, Saccharomyces cerevisiae, Transcription, Genetic, Open Reading Frames, Genome, Fungal

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