The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Tissue factor- and factor X-dependent activation of protease-activated receptor 2 by factor VIIa
Open accessAbstract
Protease-activated receptor 2 (PAR2) is expressed by vascular endothelial cells and other cells in which its function and physiological activator(s) are unknown. Unlike PAR1, PAR3, and PAR4, PAR2 is not activatable by thrombin. Coagulation factors VIIa (FVIIa) and Xa (FXa) are proteases that act upstream of thrombin in the coagulation cascade and require cofactors to interact with their substrates. These proteases elicit cellular responses, but their receptor(s) have not been identified. We asked whether FVIIa and FXa might activate PARs if presented by their cofactors. Co-expression of tissue factor (TF), the cellular cofactor for FVIIa, together with PAR1, PAR2, PAR3, or PAR4 conferred TF-dependent FVIIa activation of PAR2 and, to lesser degree, PAR1. Responses to FXa were also observed but were independent of exogenous cofactor. The TF/FVIIa complex converts the inactive zymogen Factor X (FX) to FXa. Strikingly, when FX was present, low picomolar concentrations of FVIIa caused robust signaling in cells expressing TF and PAR2. Responses in keratinocytes and cytokine-treated endothelial cells suggested that PAR2 may be activated directly by TF/FVIIa and indirectly by TF/FVIIa-generated FXa at naturally occurring expression levels of TF and PAR2. These results suggest that PAR2, although not activatable by thrombin, may nonetheless function as a sensor for coagulation proteases and contribute to endothelial activation in the setting of injury and inflammation. More generally, these findings highlight the potential importance of cofactors in regulating PAR function and specificity.
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Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line.
Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., & Fusenig, N. E. (1988) · The Journal of cell biology · doi:10.1083/jcb.106.3.761 · Cited by 4,170
Vu, T.-K. H., Hung, D. T., Wheaton, V. I., & Coughlin, S. R. (1991) · Cell · doi:10.1016/0092-8674(91)90261-v · Cited by 2,970
Self-citationDrake, T. A., Morrissey, J. H., & Edgington, T. S. (1989) · Europe PMC (PubMed Central) · Cited by 1,192
Bevilacqua, M. P., Pober, J. S., Majeau, G. R., Fiers, W., Cotran, R. S., & Gimbrone, M. A. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.12.4533 · Cited by 1,067
A dual thrombin receptor system for platelet activation
Kahn, M. L., Zheng, Y.-W., Huang, W., Bigornia, V., Zeng, D., Moff, S., Farese, R. V., Tam, C., & Coughlin, S. R. (1998) · Nature · doi:10.1038/29325 · Cited by 979
Self-citationAgonists of proteinase-activated receptor 2 induce inflammation by a neurogenic mechanism
Steinhoff, M., Vergnolle, N., Young, S. H., Tognetto, M., Amadesi, S., Ennes, H. S., Trevisani, M., Hollenberg, M. D., Wallace, J. L., Caughey, G. H., et al. (2000) · Nature Medicine · doi:10.1038/72247 · Cited by 936
Molecular cloning of a potential proteinase activated receptor.
Nystedt, S., Emilsson, K., Wahlestedt, C., & Sundelin, J. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.20.9208 · Cited by 886
Protease-activated receptor 3 is a second thrombin receptor in humans
Ishihara, H., Connolly, A. J., Zeng, D., Kahn, M. L., Wu Zheng, Y., Timmons, C., Tram, T., & Coughlin, S. R. (1997) · Nature · doi:10.1038/386502a0 · Cited by 865
Self-citationProtease-activated receptors 1 and 4 mediate activation of human platelets by thrombin
Kahn, M. L., Nakanishi-Matsui, M., Shapiro, M. J., Ishihara, H., & Coughlin, S. R. (1999) · Journal of Clinical Investigation · doi:10.1172/jci6042 · Cited by 841
Self-citationHemostasis and Thrombosis: Basic Principles and Clinical Practice
Francis, J. L. (1994) · Blood Coagulation & Fibrinolysis · doi:10.1097/00001721-199410000-00031 · Cited by 715
Cited by
Protease-Activated Receptors: Contribution to Physiology and Disease
OSSOVSKAYA, V. S., & BUNNETT, N. W. (2004) · Physiological Reviews · doi:10.1152/physrev.00028.2003 · Cited by 1,078
Activation of Endothelial Cell Protease Activated Receptor 1 by the Protein C Pathway
Riewald, M., Petrovan, R. J., Donner, A., Mueller, B. M., & Ruf, W. (2002) · Science · doi:10.1126/science.1071699 · Cited by 831
Role of Tissue Factor in Hemostasis, Thrombosis, and Vascular Development
Mackman, N. (2004) · Arteriosclerosis, Thrombosis, and Vascular Biology · doi:10.1161/01.atv.0000130465.23430.74 · Cited by 658
Proteinase-Activated Receptors
Macfarlane, S. R., Seatter, M. J., Kanke, T., Hunter, G. D., & Plevin, R. (2001) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01493-5 · Cited by 641
Infection and inflammation and the coagulation system
Levi, M. (2003) · Cardiovascular Research · doi:10.1016/s0008-6363(02)00857-x · Cited by 517
Metadata
- Authors
- Camerer, E., Huang, W., & Coughlin, S. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2000-05-09
- Volume
- 97
- Issue
- 10
- Pages
- 5255-5260
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2029539300
- Cited by
- 675
- References
- 37
- FWCI
- 23.56
- Type
- journal article
- Language
- en
- Open access
- green
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