Overproduction and Physical Characterization of SoxR, a [2Fe-2S] Protein That Governs an Oxidative Response Regulon in Escherichia coli
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SoxR protein governs the soxRS (superoxide response) regulon of Escherichia coli by becoming a transcriptional activator when the cells are exposed to compounds that mediate univalent redox reactions, many of which produce superoxide as a by-product. SoxR was overproduced and purified to near homogeneity from a strain bearing an expression vector. It could bind specifically to the soxS operator even in the absence of RNA polymerase. The aerobically purified protein, which is readily autooxidized, could activate the transcription of soxS DNA even without exposure to known inducing agents. SoxR is a globular homodimer. It contains one [2Fe-2S] cluster per polypeptide chain, as demonstrated by optical and EPR spectroscopy combined with stoichiometric analysis of iron content, unpaired-electron-spin density, and reduction by dithionite. The protein is active in its oxidized ([2Fe-2S]2+) state. The presence of a prosthetic group capable of univalent redox reactions may help to explain the activation of the regulon in vivo by compounds that can mediate such reactions.
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- Authors
- Wu, J., Dunham, W. R., & Weiss, B.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Weiss, Bernard
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-04
- Volume
- 270
- Issue
- 17
- Pages
- 10323-10327
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2025874325
- Cited by
- 103
- References
- 35
- Citations by year
- 2025: 2 · 2022: 1 · 2018: 1 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 6.65
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Center for Research Resources (M-01RR00042, M01RR000042)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- SoxR, [2Fe-2S] cluster, SOX, transcriptional activator, Escherichia coli, protein overexpression, protein purification, autooxidation, homodimer, prosthetic groups, superoxide, DNA binding, electron spin resonance
- MeSH terms
- Bacterial Proteins, Base Sequence, Cloning, Molecular, Dithionite, Electron Spin Resonance Spectroscopy, Escherichia coli, Iron-Sulfur Proteins, Metals, Molecular Sequence Data, Oxidation-Reduction, Plasmids, Transcription Factors, Sequence Homology, Amino Acid, DNA Primers, Regulon, Oxidative Stress
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