Cited by 359Redox biology and oxidative stressBacterial Genetics and BiotechnologyNitric Oxide and Endothelin Effects

Nitrosative Stress: Activation of the Transcription Factor OxyR

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Hausladen, Alfred, Privalle, Christopher T, Keng, Teresa, DeAngelo, Joseph, Stamler, Jonathan S·1996·Cell

Abstract

Hydrogen peroxide (H2O2) imposes an oxidative stress to Escherichia coli that is manifested by oxidation of glutathione and related redox-sensitive targets. OxyR is a thiol-containing transcriptional activator whose oxidation controls the expression of genes involved in H2O2 detoxification. Here we report that certain S-nitrosothiols (RSNOs) impose what we term a "nitrosative stress" to E. coli, evidenced by lowering of intracellular thiol and the transcriptional activation of OxyR by S-nitrosylation. This cellular and genetic response determines the metabolic fate of RSNOs and thereby contributes to bacterial rescue from stasis. Our studies reveal that signaling by S-nitrosylation can extend to the level of transcription and describe a metabolic pathway that constitutes an adaptation to nitrosative stress.

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Crossref · 10.1016/s0092-8674(00)80147-6

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  • Higher NO concentrations (>1 μM) can lead to oxidative stress and nitrative and nitrosative stress via peroxynitrite and nitrosating species, and in combination with oxygen can trigger posttranslational modification of proteins, lipids, and DNA.

    “Higher concentrations of NO (>1 μM) can lead not only to oxidative stress but also nitrative and nitrosative stress via the generation of peroxynitrite and nitrosating species”

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Authors
Hausladen, A., Privalle, C. T., Keng, T., DeAngelo, J., & Stamler, J. S.
Affiliations
Duke University (US)
Corresponding author
Stamler, Jonathan S
Venue
Cell
Publisher
Elsevier BV
Published
1996-09
Volume
86
Issue
5
Pages
719-729
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2007013508
Cited by
359
References
66
Citations by year
2026: 1 · 2025: 5 · 2024: 1 · 2023: 2 · 2022: 6 · 2021: 5 · 2020: 6 · 2019: 7 · 2018: 13 · 2017: 3 · 2016: 9 · 2015: 5 · 2014: 9 · 2013: 10 · 2012: 9
FWCI
7.95
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (1k08hl002582-01, 5r01hl052529-05)
  • National Heart, Lung, and Blood Institute (HL02582, HL 52529)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Keywords
nitrosative stress, OxyR, S-nitrosylation, S-nitrosothiols, hydrogen peroxide, oxidative stress, Escherichia coli, thiol oxidation, transcriptional activation, hydrogen peroxide detoxification, glutathione, bacterial adaptation
MeSH terms
Aerobiosis, Anaerobiosis, Catalase, Cysteine, DNA-Binding Proteins, Enzyme Induction, Escherichia coli, Glutathione, Hydrogen Peroxide, Mercaptoethanol, Nitric Oxide, Nitroso Compounds, Oxidation-Reduction, Repressor Proteins, Transcription Factors, Transcriptional Activation, Gene Expression Regulation, Bacterial, Oxidants, Oxidative Stress, S-Nitrosothiols, Escherichia coli Proteins

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