The DOI matches its Crossref record; 56 of the 56 references it cites were found in a registry or an index.
Processing of Transforming Growth Factor β1 Precursor by Human Furin Convertase
Open accessAbstract
Proteolytic processing of the transforming growth factor beta precursor (pro-TGF beta) is an essential step in the formation of the biologically active TGF beta homodimeric protein (TGF beta). The 361-amino-acid precursor pro-TGF beta 1 has within its primary structure the R-H-R-R processing signal found in many constitutively secreted precursor proteins and potentially recognized by members of the mammalian convertase family of endoproteases. To determine whether cleavage of pro-TGF beta 1 can be achieved by the furin convertase in vitro, purified precursor was incubated in the presence of a truncated/secreted form of the enzyme. Immunoblots showed that the 55-kDa pro-TGF beta 1 was converted into the 44 and 12.5 kDa bands corresponding to the pro-region and the mature monomer, respectively. Treatment of pro-TGF beta 1 with furin resulted in a 5-fold increase in the production of biologically active TGF beta 1. Furthermore, when expressed in the furin-deficient LoVo cells, no processing of pro-TGF beta 1 was observed. In contrast, efficient processing was observed when pro-TGF beta was coexpressed with the furin convertase. Collectively, these results provide evidence that in our experimental systems the TGF beta 1 precursor is efficiently and correctly processed by human furin thus permitting release of the biologically active peptide.
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TGF-β signaling and the fibrotic response
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Making sense of latent TGFβ activation
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BDNF and Activity-Dependent Synaptic Modulation: Figure 1.
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Genetic Analysis of the Mammalian Transforming Growth Factor-β Superfamily
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Metadata
- Authors
- Dubois, C. M., Laprise, M.-H., Blanchette, F., Gentry, L. E., & Leduc, R.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-05
- Volume
- 270
- Issue
- 18
- Pages
- 10618-10624
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1993531220
- Cited by
- 405
- References
- 56
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Dubois, C. M., Laprise, M.-H., Blanchette, F., Gentry, L. E., & Leduc, R. (1995). Processing of Transforming Growth Factor β1 Precursor by Human Furin Convertase. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.18.10618
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
