The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Gα12 and Gα13 Stimulate Rho-dependent Stress Fiber Formation and Focal Adhesion Assembly
Open accessAbstract
Rho, a member of the Ras superfamily of GTP-binding proteins, regulates actin polymerization resulting in the formation of stress fibers and the assembly of focal adhesions. In Swiss 3T3 cells, heterotrimeric G protein-coupled receptors for lysophosphatidic acid and gastrin releasing peptide stimulate Rho-dependent stress fiber and focal adhesion formation. The specific heterotrimeric G protein subunits mediating Rho-dependent stress fiber and focal adhesion formation have not been defined previously. We have expressed GTPase-deficient, constitutively activated G protein alpha subunits and mixtures of beta and gamma subunits in Swiss 3T3 cells. Measurement of actin polymerization and focal adhesion formation indicated that GTPase-deficient alpha 12 and alpha 13, but not the activated forms of alpha 12 or alpha q stimulated stress fiber and focal adhesion assembly. Combinations of beta and gamma subunits were unable to stimulate stress fiber or focal adhesion formation. G alpha 12- and alpha 13-mediated stress fiber and focal adhesion assembly was inhibited by botulinum C3 exoenzyme, which ADP-ribosylates and inactivates Rho, indicating that alpha 12 and alpha 13, but not other G protein alpha subunits or beta gamma complexes, regulate Rho-dependent responses. The results define the integration of G12 and G13 with the regulation of the actin cytoskeleton.
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Cited by
G-protein-coupled receptors and signaling networks: emerging paradigms
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p115 RhoGEF, a GTPase Activating Protein for Gα 12 and Gα 13
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Metadata
- Authors
- Buhl, A. M., Johnson, N. L., Dhanasekaran, N., & Johnson, G. L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-10
- Volume
- 270
- Issue
- 42
- Pages
- 24631-24634
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2077537799
- Cited by
- 448
- References
- 34
- FWCI
- 8.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Buhl, A. M., Johnson, N. L., Dhanasekaran, N., & Johnson, G. L. (1995). Gα12 and Gα13 Stimulate Rho-dependent Stress Fiber Formation and Focal Adhesion Assembly. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.42.24631
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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