The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Transport of Metal-binding Peptides by HMT1, A Fission Yeast ABC-type Vacuolar Membrane Protein
Open accessAbstract
The Schizosaccharomyces pombe hmt1 gene encodes an ABC (ATP-binding cassette)-type protein essential for Cd2+ tolerance. Immunoblot analysis of subcellular fractions indicates that the native HMT1 polypeptide is associated with the vacuolar membrane. Vacuolar membrane vesicles were purified from strains that hyperproduce, or are deficient in, the HMT1 protein. In vitro transport of radiolabeled substrates by these vesicles indicates that HMT1 is an ATP-dependent transporter of phytochelatins, the metal-chelating peptides involved in heavy metal tolerance of plants and certain fungi. Vacuolar vesicles containing HMT1 are capable of taking up both apo-phytochelatins and phytochelatin-Cd2+ complexes. HMT1 activity is sensitive to antibodies directed against this protein and to vanadate, but not to inhibitors affecting the vacuolar proton ATPase or ionophores that abolish the pH gradient across the vacuolar membrane. Vacuolar uptake of Cd2+ and of a glutathione conjugate were also observed, but are not attributable to HMT1. These studies highlight the importance of the yeast vacuole in detoxification of xenobiotics.
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Balzi, E., Wang, M., Leterme, S., Van Dyck, L., & Goffeau, A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42155-7 · Cited by 447
Kamijo, K., Taketani, S., Yokota, S., Osumi, T., & Hashimoto, T. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)39595-x · Cited by 391
Cited by
P HYTOCHELATINS AND M ETALLOTHIONEINS : Roles in Heavy Metal Detoxification and Homeostasis
Cobbett, C., & Goldsbrough, P. (2002) · Annual Review of Plant Biology · doi:10.1146/annurev.arplant.53.100301.135154 · Cited by 2,518
Molecular mechanisms of plant metal tolerance and homeostasis
Clemens, S. (2001) · Planta · doi:10.1007/s004250000458 · Cited by 1,417
A long way ahead: understanding and engineering plant metal accumulation
Clemens, S., Palmgren, M. G., & Krämer, U. (2002) · Trends in Plant Science · doi:10.1016/s1360-1385(02)02295-1 · Cited by 1,265
Phytoremediation of soil metals
Chaney, R. L., Malik, M., Li, Y. M., Brown, S. L., Brewer, E. P., Angle, J. S., & Baker, A. J. (1997) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(97)80004-3 · Cited by 1,254
Arsenic uptake and metabolism in plants
Zhao, F. J., Ma, J. F., Meharg, A. A., & McGrath, S. P. (2009) · New Phytologist · doi:10.1111/j.1469-8137.2008.02716.x · Cited by 1,185
Metadata
- Authors
- Ortiz, D. F., Ruscitti, T., McCue, K. F., & Ow, D. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1995-03
- Volume
- 270
- Issue
- 9
- Pages
- 4721-4728
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2025327528
- Cited by
- 470
- References
- 2
- FWCI
- 6.33
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Ortiz, D. F., Ruscitti, T., McCue, K. F., & Ow, D. W. (1995). Transport of Metal-binding Peptides by HMT1, A Fission Yeast ABC-type Vacuolar Membrane Protein. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.270.9.4721
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