The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Distinct Roles in Signal Transduction for Each of the Phospholipase A2 Enzymes Present in P388D1 Macrophages
Open accessAbstract
Receptor-stimulated arachidonic acid (AA) mobilization in P388D1 macrophages consists of a transient phase in which AA accumulates in the cell and a sustained phase in which AA accumulates in the incubation medium. We have shown previously that a secretory group II phospholipase A2 (sPLA2) is the enzyme responsible for most of the AA released to the incubation medium. By using selective inhibitors for each of the PLA2s present in P388D1 macrophages, we demonstrate herein that the cytosolic group IV PLA2 (cPLA2) mediates accumulation of cell-associated AA during the early steps of P388D1 cell activation. The contribution of both cPLA2 and sPLA2 to AA release can be distinguished on the basis of the different spatial and temporal characteristics of activation and substrate preferences of the two phospholipase A2s (PLA2s). Furthermore, the results suggest the possibility that a functionally active cPLA2 may be necessary for sPLA2 to act. cPLA2 action precedes that of sPLA2, and overcoming cPLA2 inhibition by artificially increasing intracellular free AA levels restores extracellular AA release. Although this suggests cross-talk between cPLA2 and sPLA2, selective inhibition of one other PLA2 present in these cells, namely the Ca2+-independent PLA2, does not block, but instead enhances receptor-coupled AA release. These data indicate that Ca2+-independent PLA2 does not mediate AA mobilization in P388D1 macrophages. Collectively, the results of this work suggest that each of the PLA2s present in P388D1 macrophages serves a distinct role in cell activation and signal transduction.
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Cites
A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION
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cPLA2 is phosphorylated and activated by MAP kinase
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Diversity of group types, regulation, and function of phospholipase A2.
Dennis, E. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36794-7 · Cited by 1,436
Self-citationFourcade, O., Simon, M.-F., Viodé, C., Rugani, N., Leballe, F., Ragab, A., Fournié, B., Sarda, L., & Chap, H. (1995) · Cell · doi:10.1016/0092-8674(95)90295-3 · Cited by 536
Ackermann, E. J., Conde-Frieboes, K., & Dennis, E. A. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.1.445 · Cited by 393
Self-citationMammalian non-pancreatic phospholipases A2
Kudo, I., Murakami, M., Hara, S., & Inoue, K. (1993) · Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism · doi:10.1016/0005-2760(93)90003-r · Cited by 351
Transbilayer movement of phospholipids in red cell and platelet membranes
Schroit, A. J., & Zwaal, R. F. A. (1991) · Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes · doi:10.1016/0304-4157(91)90019-s · Cited by 313
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Balsinde, J., Bianco, I. D., Ackermann, E. J., Conde-Frieboes, K., & Dennis, E. A. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.18.8527 · Cited by 287
Self-citationAckermann, E. J., Kempner, E. S., & Dennis, E. A. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37098-9 · Cited by 266
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Cited by
Lauber, K., Bohn, E., Kröber, S. M., Xiao, Y.-J., Blumenthal, S. G., Lindemann, R. K., Marini, P., Wiedig, C., Zobywalski, A., Baksh, S., et al. (2003) · Cell · doi:10.1016/s0092-8674(03)00422-7 · Cited by 967
The role of omega-3 long-chain polyunsaturated fatty acids in health and disease of the retina
SanGiovanni, J. P., & Chew, E. Y. (2005) · Progress in Retinal and Eye Research · doi:10.1016/j.preteyeres.2004.06.002 · Cited by 874
REGULATION AND INHIBITION OF PHOSPHOLIPASE A2
Balsinde, J., Balboa, M. A., Insel, P. A., & Dennis, E. A. (1999) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.39.1.175 · Cited by 566
Self-citationPhospholipase A2 regulation of arachidonic acid mobilization
Balsinde, J., Winstead, M. V., & Dennis, E. A. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)03413-0 · Cited by 477
Self-citationKurrasch‐Orbaugh, D. M., Parrish, J. C., Watts, V. J., & Nichols, D. E. (2003) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.2003.01921.x · Cited by 138
Metadata
- Authors
- Balsinde, J., & Dennis, E. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-03
- Volume
- 271
- Issue
- 12
- Pages
- 6758-6765
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2041299660
- Cited by
- 351
- References
- 28
- FWCI
- 22.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
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