The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Overexpression of Calreticulin Increases Intracellular Ca2+ Storage and Decreases Store-operated Ca2+ Influx
Open accessAbstract
The widely distributed and highly conserved Ca(2+)-binding protein calreticulin has been suggested to play a role as a Ca2+ storage protein of intracellular Ca+ stores. To test this hypothesis, we have generated a mouse L fibroblast cell line stably transfected with a calreticulin expression vector. The calreticulin content of the overexpressers was increased by 1.6 +/- 0.2-fold compared with mock-transfected cells. The total cellular Ca2+ content of calreticulin-overexpressing and control cells, as assessed by equilibrium 45Ca+2 uptake, was 141 +/- 8 and 67 +/- 6 pmol of Ca2+/10(6) cells, respectively (i.e. a 2.1 +/- 0.2-fold increase in the Ca2+ content of calreticulin-overexpressing cells). Over 80% of the increased Ca2+ content was found within thapsigargin-sensitive Ca2+ stores. The pattern of calreticulin distribution, revealed by immunofluorescence microscopy, showed an endoplasmic reticulum-like pattern and was identical in overexpressers and control cells. In overexpressers, cytosolic free [Ca2+] elevations due to Ca2+ release were enhanced when either ATP or a combination of ionomycin and thapsigargin was used as a stimulus. In contrast, thapsigargin-induced Ca2+ and Mn2+ influxes from the extracellular space were markedly diminished in calreticulin-overexpressing cells, suggesting an active involvement of calreticulin in the regulation of store-operated Ca2+ influx.
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Store-Operated Calcium Channels
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Metadata
- Authors
- Mery, L., Mesaeli, N., Michalak, M., Opas, M., Lew, D. P., & Krause, K.-H.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1996-04
- Volume
- 271
- Issue
- 16
- Pages
- 9332-9339
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1600052472
- Cited by
- 271
- References
- 19
- FWCI
- 10.53
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Mery, L., Mesaeli, N., Michalak, M., Opas, M., Lew, D. P., & Krause, K.-H. (1996). Overexpression of Calreticulin Increases Intracellular Ca2+ Storage and Decreases Store-operated Ca2+ Influx. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.271.16.9332
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