Interaction of the Grb10 Adapter Protein with the Raf1 and MEK1 Kinases

Nantel, André; Mohammad-Ali, Khosro; Sherk, Jennifer; Posner, Barry I.; Thomas, David Y.

doi:10.1074/jbc.273.17.10475

Cited by 108Protein Kinase Regulation and GTPase SignalingProtein Tyrosine PhosphatasesMelanoma and MAPK Pathways

Interaction of the Grb10 Adapter Protein with the Raf1 and MEK1 Kinases

Open access

Nantel, André, Mohammad-Ali, Khosro, Sherk, Jennifer, Posner, Barry I., Thomas, David Y.·1998·Journal of Biological Chemistry

DOI 10.1074/jbc.273.17.10475

Open this paper in CoChat — free account Open PDF

Abstract

Grb10 and its close homologues Grb7 and Grb14, belong to a family of adapter proteins characterized by a proline-rich region, a central PH domain, and a carboxyl-terminal Src homology 2 (SH2) domain. Their interaction with a variety of activated tyrosine kinase receptors is well documented, but their actual function remains a mystery. The Grb10 SH2 domain was isolated from a two-hybrid screen using the MEK1 kinase as a bait. We show that this unusual SH2 domain interacts, in a phosphotyrosine-independent manner, with both the Raf1 and MEK1 kinases. Mutation of the MEK1 Thr-386 residue, which is phosphorylated by mitogen-activated protein kinase in vitro, reduces binding to Grb10 in a two-hybrid assay. Interaction of Grb10 with Raf1 is constitutive, while interaction between Grb10 and MEK1 needs insulin treatment of the cells and follows mitogen-activated protein kinase activation. Random mutagenesis of the SH2 domain demonstrated that the Arg-betaB5 and Asp-EF2 residues are necessary for binding to the epidermal growth factor and insulin receptors as well as to the two kinases. In addition, we show that a mutation in Ser-betaB7 affects binding only to the receptors, while a mutation in Thr-betaC5 abrogates binding only to MEK1. Finally, transfection of Grb10 genes with specific mutations in their SH2 domains induces apoptosis in HTC-IR and COS-7 cells. These effects can be competed by co-expression of the wild type protein, suggesting that these mutants act by sequestering necessary signaling components.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1074/jbc.273.17.10475

Cites

71 of 71 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Opposing Effects of ERK and JNK-p38 MAP Kinases on Apoptosis
Xia, Z., Dickens, M., Raingeaud, J., Davis, R. J., & Greenberg, M. E. (1995) · Science · doi:10.1126/science.270.5240.1326 · Cited by 5,290 · see citing works
Basic Methods in Molecular Biology
(1986) · Elsevier eBooks · doi:10.1016/b978-0-444-01082-7.x5001-2 · Cited by 3,163 · see citing works
Mitogen-activated protein kinase pathways
Robinson, M. J., & Cobb, M. H. (1997) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(97)80061-0 · Cited by 2,495 · see citing works
Current Protocols in Molecular Biology
Teplitz, R. L. (1989) · JAMA · doi:10.1001/jama.1989.03420090114049 · Cited by 1,848 · see citing works
Independent Human MAP-Kinase Signal Transduction Pathways Defined by MEK and MKK Isoforms
Dérijard, B., Raingeaud, J., Barrett, T., Wu, I.-H., Han, J., Ulevitch, R. J., & Davis, R. J. (1995) · Science · doi:10.1126/science.7839144 · Cited by 1,555 · see citing works
Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2
Gyuris, J. (1993) · Cell · doi:10.1016/0092-8674(93)90498-f · Cited by 1,534 · see citing works
Yeast promoters and lacZ fusions designed to study expression of cloned genes in yeast.
Guarente, L. P. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)01013-7 · Cited by 1,022 · see citing works
Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun
Sánchez, I., Hughes, R. T., Mayer, B. J., Yee, K. O., Woodgett, J. R., Avruch, J., Kyriakls, J. M., & Zon, L. I. (1994) · Nature · doi:10.1038/372794a0 · Cited by 970 · see citing works
Localization of Protein Kinases by Anchoring Proteins: a Theme in Signal Transduction
Mochly‐Rosen, D. (1995) · Science · doi:10.1126/science.7716516 · Cited by 905 · see citing works
Identification of a Dual Specificity Kinase That Activates the Jun Kinases and p38-Mpk2
Lin, A., Minden, A., Martinetto, H., Claret, F.-X., Lange, C. A., Mercurio, F., Johnson, G. L., & Karin, M. (1995) · Science · doi:10.1126/science.7716521 · Cited by 763 · see citing works
Binding of a high affinity phosphotyrosyl peptide to the Src SH2 domain: Crystal structures of the complexed and peptide-free forms
Waksman, G. (1993) · Cell · doi:10.1016/0092-8674(93)90405-f · Cited by 747 · see citing works
Oxidative stress activates extracellular signal-regulated kinases through Src and Ras in cultured cardiac myocytes of neonatal rats.
Aikawa, R., Komuro, I., Yamazaki, T., Zou, Y., Kudoh, S., Tanaka, M. H., Shiojima, I., Hiroi, Y., & Yazaki, Y. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119709 · Cited by 687 · see citing works
Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides
Waksman, G., Kominos, D., Robertson, S. C., Pant, N., Baltimore, D., Birge, R. B., Cowburn, D., Hanafusa, H., Mayer, B. J., Overduin, M., et al. (1992) · Nature · doi:10.1038/358646a0 · Cited by 675 · see citing works
SH2-Containing Phosphotyrosine Phosphatase as a Target of Protein-Tyrosine Kinases
Feng, G., Hui, C.-C., & Pawson, T. J. (1993) · Science · doi:10.1126/science.8096088 · Cited by 673 · see citing works
Insulin-like Growth Factor 1 Inhibits Apoptosis Using the Phosphatidylinositol 3′-Kinase and Mitogen-activated Protein Kinase Pathways
Párrizas, M., Saltiel, A. R., & LeRoith, D. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.1.154 · Cited by 653 · see citing works
Coordination of Three Signaling Enzymes by AKAP79, a Mammalian Scaffold Protein
Klauck, T. M., Faux, M. C., Labudda, K., Langeberg, L. K., Jaken, S., & Scott, J. D. (1996) · Science · doi:10.1126/science.271.5255.1589 · Cited by 550 · see citing works
Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae
Chol, K.-Y., Satterberg, B., Lyons, D. M., & Elion, E. A. (1994) · Cell · doi:10.1016/0092-8674(94)90427-8 · Cited by 502 · see citing works
Recognition of a high-affinity phosphotyrosyl peptide by the Src homology-2 domain of p56lck
Eck, M. J., Shoelson, S. E., & Harrison, S. C. (1993) · Nature · doi:10.1038/362087a0 · Cited by 499 · see citing works
Kinase Suppressor of Ras Is Ceramide-Activated Protein Kinase
Zhang, Y., Yao, B., Delikat, S. E., Bayoumy, S. S., Lin, X., Basu, S., McGinley, M., Chan-Hui, P.-Y., Lichenstein, H. S., & Kolesnick, R. (1997) · Cell · doi:10.1016/s0092-8674(00)80183-x · Cited by 437 · see citing works
BCR sequences essential for transformation by the BCR-ABL oncogene bind to the ABL SH2 regulatory domain in a non-phosphotyrosine-dependent manner
Pendergast, A. M., Muller, A. J., Havlik, M. H., et al. (1991) · Cell · doi:10.1016/0092-8674(91)90148-r · Cited by 376 · see citing works
Rotation of photoreceptor clusters in the developing drosophila eye requires the nemo gene
Choi, K.-W., & Benzer, S. (1994) · Cell · doi:10.1016/0092-8674(94)90579-7 · Cited by 339 · see citing works
Identification of the major phosphorylation sites of the Raf-1 kinase
Morrison, D. K., Heidecker, G., Rapp, U. R., & Copeland, T. D. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85336-x · Cited by 328 · see citing works
Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3
Nishina, H., Fischer, K., Radvanyi, L. G., Shahinian, A., Hakem, R., Rubie, E. A., Bernstein, A., Mak, T. W., Woodgett, J. R., & Penninger, J. M. (1997) · Nature · doi:10.1038/385350a0 · Cited by 323 · see citing works
The pleckstrin homology domain: An intriguing multifunctional protein module
Shaw, G. (1996) · BioEssays · doi:10.1002/bies.950180109 · Cited by 281 · see citing works
The adaptor protein Nck links receptor tyrosine kinases with the serine-threonine kinase Pak1
Galisteo, M. L., Chernoff, J., Su, Y. C., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.35.20997 · Cited by 274 · see citing works
Direct Random Mutagenesis of Gene-Sized DNA Fragments Using Polymerase Chain Reaction
Fromant, M., Blanquet, S., & Plateau, P. (1995) · Analytical Biochemistry · doi:10.1006/abio.1995.1050 · Cited by 273 · see citing works
Molecular Glue: Kinase Anchoring and Scaffold Proteins
Faux, M. C., & Scott, J. D. (1996) · Cell · doi:10.1016/s0092-8674(00)81075-2 · Cited by 269 · see citing works
Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein
Nauert, J. B., Klauck, T. M., Langeberg, L. K., & Scott, J. D. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00027-3 · Cited by 256 · see citing works
The SH2 domain protein GRB‐7 is co‐amplified, overexpressed and in a tight complex with HER2 in breast cancer.
Stein, D., Wu, J., Fuqua, S. A. W., et al. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06386.x · Cited by 249 · see citing works
Fibroblast Growth Factor-2 Suppression of Tumor Necrosis Factor α-Mediated Apoptosis Requires Ras and the Activation of Mitogen-activated Protein Kinase
Gardner, A. M., & Johnson, G. L. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.24.14560 · Cited by 215 · see citing works
Labelling DNA strand breaks with BrdUTP. Detection of apoptosis and cell proliferation
Li, X., & Darżynkiewicz, Z. (1995) · Cell Proliferation · doi:10.1111/j.1365-2184.1995.tb00045.x · Cited by 209 · see citing works
A differential efficiency of adenovirus-mediated in vivo gene transfer into skeletal muscle cells of different maturity
Acsádi, G., Jáni, Á., Bernard Massie, B., Simoneau, M., Holland, P. C., Blaschuk, K., & Karpati, G. (1994) · Human Molecular Genetics · doi:10.1093/hmg/3.4.579 · Cited by 207 · see citing works
Crystal structures of peptide complexes of the amino-terminal SH2 domain of the Syp tyrosine phosphatase
Lee, C., Kominos, D., Jacques, S. L., Margolis, B. L., Schlessinger, J., Shoelson, S. E., & Kuriyan, J. (1994) · Structure · doi:10.1016/s0969-2126(00)00044-7 · Cited by 205 · see citing works
Pheromone Response in Yeast: Association of Bem1p with Proteins of the MAP Kinase Cascade and Actin
Leeuw, T., Fourest‐Lieuvin, A., Wu, C., Chenevert, J. M., Clark, K., Whiteway, M. S., Thomas, D. Y., & Leberer, E. (1995) · Science · doi:10.1126/science.270.5239.1210 · Cited by 204 · see citing works
Self-citation
Properties of MEKs, the kinases that phosphorylate and activate the extracellular signal-regulated kinases.
Zheng, C. F., & Guan, K. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)80474-8 · Cited by 200 · see citing works
Regulation of the MAP kinase pathway by mammalian Ksr through direct interaction with MEK and ERK
Yu, W., Fantl, W. J., Harrowe, G., & Williams, L. T. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70020-x · Cited by 197 · see citing works
Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain.
Joung, I., Strominger, J. L., & Shin, J. H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.5991 · Cited by 194 · see citing works
The protein kinase KSR interacts with 14-3-3 protein and Raf
Xing, H., Kornfeld, K., & Muslin, A. J. (1997) · Current Biology · doi:10.1016/s0960-9822(06)00152-7 · Cited by 173 · see citing works
High-efficiency expression/cloning of epidermal growth factor-receptor-binding proteins with Src homology 2 domains.
Margolis, B. L., Silvennoinen, O., Comoglio, F., et al. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.19.8894 · Cited by 171 · see citing works
Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function.
Liu, F., & Roth, R. A. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.22.10287 · Cited by 171 · see citing works
Association of the Yeast Pheromone Response G Protein βγ Subunits with the Map Kinase Scaffold Ste5p
Whiteway, M. S., Wu, C., Leeuw, T., Clark, K., Fourest‐Lieuvin, A., Thomas, D. Y., & Leberer, E. (1995) · Science · doi:10.1126/science.7667635 · Cited by 170 · see citing works
Self-citation
The Yeast Tribrid System—Genetic Detection of trans-phosphorylated ITAM-SH2-Interactions
Osborne, M. A., Dalton, S., & Kochan, J. P. (1995) · Nature Biotechnology · doi:10.1038/nbt1295-1474 · Cited by 164 · see citing works
Murine Ksr interacts with MEK and inhibits Ras-induced transformation
Denouel-Galy, A., Douville, E. M. J., Warne, P. H., Papin, C., Laugier, D., Calothy, G., Downward, J., & Eychène, A. (1998) · Current Biology · doi:10.1016/s0960-9822(98)70019-3 · Cited by 150 · see citing works
Ligand Activation of ELK Receptor Tyrosine Kinase Promotes Its Association with Grb10 and Grb2 in Vascular Endothelial Cells
Stein, E., Cerretti, D. P., & Daniel, T. O. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.38.23588 · Cited by 141 · see citing works
Interaction of a GRB-IR Splice Variant (a Human GRB10 Homolog) with the Insulin and Insulin-like Growth Factor I Receptors
O’Neill, T. J., Rose, D. W., Pillay, T. S., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.37.22506 · Cited by 139 · see citing works
Cloning and Characterization of GRB14, a Novel Member of the GRB7 Gene Family
Daly, R. J., Sanderson, G. M., Janes, P. W., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.21.12502 · Cited by 130 · see citing works
The cloning of Grb10 reveals a new family of SH2 domain proteins.
Ooi, J., Yajnik, V., Immanuel, D., Gordon, M., Moskow, J. J., Buchberg, A. M., & Margolis, B. L. (1995) · PubMed · Cited by 128 · see citing works
Human GRB-IRβ/GRB10
Frantz, J. D., Giorgetti‐Peraldi, S., Ottinger, E. A., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.5.2659 · Cited by 119 · see citing works
Evidence for the direct interaction of the insulin-like growth factor I receptor with IRS-1, Shc, and Grb10.
Dey, B. R., Frick, K., Lopaczyński, W., et al. (1996) · Molecular Endocrinology · doi:10.1210/mend.10.6.8776723 · Cited by 107 · see citing works
Growth factor‐stimulated MAP kinase induces rapid retrophosphorylation and inhibition of MAP kinase kinase (MEK1)
Brunet, A., Pagès, G., & Pouysségur, J. (1994) · FEBS Letters · doi:10.1016/0014-5793(94)00475-7 · Cited by 105 · see citing works
Evidence that extracellular signal-regulated kinases are the insulin-activated Raf-1 kinase kinases.
Lee, R., Cobb, M. H., & Blackshear, P. J. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)48399-8 · Cited by 104 · see citing works
Direct Association between the Ret Receptor Tyrosine Kinase and the Src Homology 2-containing Adapter Protein Grb7
Pandey, A., Liu, X., Dixon, J. E., et al. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.18.10607 · Cited by 102 · see citing works
Mitogen-Activated Protein (MAP) Kinase Phosphorylation of MAP Kinase Kinase: Determination of Phosphorylation Sites by Mass Spectrometry and Site-Directed Mutagenesis1
Mansour, S. J., Resing, K. A., Candi, J. M., Hermann, A. S., Gloor, J. W., Herskind, K. R., Wartmann, M., Davis, R. J., & Ahn, N. G. (1994) · The Journal of Biochemistry · doi:10.1093/oxfordjournals.jbchem.a124524 · Cited by 98 · see citing works
The adapter protein Grb10 associates preferentially with the insulin receptor as compared with the IGF-I receptor in mouse fibroblasts.
Laviola, L., Giorgino, F., Chow, J. C., Baquero, J. A. G., Hansen, H. F., Ooi, J., Zhu, J., Riedel, H., & Smith, R. J. (1997) · Journal of Clinical Investigation · doi:10.1172/jci119246 · Cited by 98 · see citing works
Feedback regulation of mitogen-activated protein kinase kinase kinase activity of c-Raf-1 by insulin and phorbol ester stimulation.
Ueki, K., Matsuda, S., Tobe, K., Gotoh, Y., Tamemoto, H., Yachi, M., Akanuma, Y., Yazaki, Y., Nishida, E., & Kadowaki, T. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40745-9 · Cited by 97 · see citing works
Raf-1 interacts with Fyn and Src in a non-phosphotyrosine-dependent manner
Cleghon, V., & Morrison, D. K. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)32504-8 · Cited by 94 · see citing works
Characterization of protein tyrosine phosphatase SH-PTP2. Study of phosphopeptide substrates and possible regulatory role of SH2 domains.
Dechert, U., Adam, M., Harder, K. W., et al. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)37504-x · Cited by 94 · see citing works
Coexpression of Grb7 with epidermal growth factor receptor or Her2/erbB2 in human advanced esophageal carcinoma.
Tanaka, S., Mori, M., Akiyoshi, T., et al. (1997) · PubMed · Cited by 92 · see citing works
Cloning of a Grb2 Isoform with Apoptotic Properties
Fath, I., Schweighoffer, F., Rey, I., Multon, M.-C., Boiziau, J., Duchesne, M., & Tocqué, B. (1994) · Science · doi:10.1126/science.8178156 · Cited by 91 · see citing works
The Role of mGrb10α in Insulin-like Growth Factor I-mediated Growth
Morrione, A., Valentinis, B., Resnicoff, M., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.42.26382 · Cited by 89 · see citing works
Grb7 is a Downstream Signaling Component of Platelet-derived Growth Factor α- and β-Receptors
Yokote, K., Margolis, B. L., Heldin, C., & Claesson, L. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.48.30942 · Cited by 82 · see citing works
Structural Determinants of the Interaction between the erbB2 Receptor and the Src Homology 2 Domain of Grb7
Janes, P. W., Lackmann, M., Church, W. B., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.13.8490 · Cited by 81 · see citing works
SH2/SH3 Adaptor Proteins Can Link Tyrosine Kinases to a Ste20-related Protein Kinase, HPK1
Anafi, M., Kiefer, F., Gish, G. D., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.44.27804 · Cited by 78 · see citing works
A limited set of SH2 domains binds BCR through a high-affinity phosphotyrosine-independent interaction.
Muller, A. J., Pendergast, A. M., Havlik, M. H., et al. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.11.5087 · Cited by 78 · see citing works
Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1.
Charest, D. L., Mordret, G. P., Harder, K. W., et al. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.8.4679 · Cited by 72 · see citing works
Cloning, Chromosome Localization, Expression, and Characterization of an Src Homology 2 and Pleckstrin Homology Domain-containing Insulin Receptor Binding Protein hGrb10γ
Dong, L., Du, H., Porter, S. G., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.46.29104 · Cited by 71 · see citing works
In Vivo Association of v-Abl with Shc Mediated by a Non-phosphotyrosine-dependent SH2 Interaction
Raffel, G. D., Parmar, K., & Rosenberg, N. E. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.9.4640 · Cited by 64 · see citing works
Insulin down-regulates insulin receptor number and up-regulates insulin receptor affinity in cells expressing a tyrosine kinase-defective insulin receptor.
Sbraccia, P., Wong, K.-Y., Brunetti, A., et al. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)34059-1 · Cited by 45 · see citing works
High Level Expression in 293 Cells of the Herpes Simplex Virus Type 2 Ribonucleotide Reductase Subunit 2 Using an Adenovirus Vector
Lamarche, N., Massie, B., Richer, M., et al. (1990) · Journal of General Virology · doi:10.1099/0022-1317-71-8-1785 · Cited by 28 · see citing works
Genetic analysis of a phosphatidylinositol 3-kinase SH2 domain reveals determinants of specificity.
Yoakim, M., Hou, W., Songyang, Z., et al. (1994) · Molecular and Cellular Biology · doi:10.1128/mcb.14.9.5929 · Cited by 26 · see citing works
[33] Ras-Raf complexes in Vitro
Zhang, X., Marshall, M. S., & Avruch, J. (1995) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(95)55035-6 · Cited by 7 · see citing works

Flagged first, then by citations

Research works printed but not linked: 3

Margolis, B., Silvennoinen, O., Comoglio, F., Roonprapunt, C., Skolnik, E., Ullrich, A., and Schlessinger, J. (1992) Proc. Natl. Acad. Sci. U. S. A. 89, 8894 -8898
Liu, F., and Roth, R. A. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 10287-10291
Joung, I., Strominger, J. L., and Shin, J. (1996) Proc. Natl. Acad. Sci. U. S. A. 93, 5991-5995

Sorted by citations

Quantifying the contribution of Neanderthal introgression to the heritability of complex traits
McArthur, E., Rinker, D. C., & Capra, J. A. (2021) · Nature Communications · doi:10.1038/s41467-021-24582-y · Cited by 71 · see citing works
Tissue-specific regulation and function of Grb10 during growth and neuronal commitment
Plasschaert, R. N., & Bartolomei, M. S. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1411254111 · Cited by 67 · see citing works

Authors: Nantel, A., Mohammad-Ali, K., Sherk, J., Posner, B. I., & Thomas, D. Y.
Affiliations: Biotechnology Research Institute (CA)
McGill University (CA)
Corresponding author: Nantel, André
ORCID: Nantel, André
Thomas, David Y.
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1998-04
Volume: 273
Issue: 17
Pages: 10475-10484
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1074/jbc.273.17.10475
OpenAlex: W2046462167
Cited by: 108
References: 71
Citations by year: 2025: 1 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 4 · 2013: 3 · 2012: 2
FWCI: 3.77
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 8238 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by)
PubMed (publishedVersion)
NPARC (acceptedVersion)
INFM-OAR (INFN Catania) (submittedVersion, cc-by)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Tyrosine Phosphatases
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Melanoma and MAPK Pathways
Keywords: Grb10, adaptor proteins, MEK1, SH2 domain, protein-protein interaction, mitogen-activated protein kinase, epidermal growth factor receptor, insulin receptor, proline-rich region, apoptosis, Grb7, Grb14
MeSH terms: Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Cell Line, DNA, Humans, Molecular Sequence Data, Phosphorylation, Protein Binding, Protein-Tyrosine Kinases, Proteins, Sequence Homology, Nucleic Acid, Apoptosis, Protein Serine-Threonine Kinases, Point Mutation, Sequence Homology, Amino Acid, Proto-Oncogene Proteins c-raf, Mitogen-Activated Protein Kinase Kinases, MAP Kinase Kinase 1, GRB10 Adaptor Protein

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Interaction of the Grb10 Adapter Protein with the Raf1 and MEK1 Kinases

Abstract

Integrity signals

Claims the paper makes

Self-check

Cites

Cited by

Related

Record

About these checks