The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Two Kinesin Light Chain Genes in Mice
Open accessAbstract
Native kinesin consists of two light chains and two heavy chains in a 1:1 stoichiometric ratio. To date, only one gene for kinesin light chain has been characterized, while a second gene was identified in a genomic sequencing study but not analyzed biochemically. Here we describe new genes encoding kinesin light chains in mouse. One of these light chains is neuronally enriched, while another shows ubiquitous expression. The presence of multiple kinesin light chain genes in mice is especially interesting, since there are two kinesin heavy chain genes in humans (Niclas, J., Navone, F., Hom-Booher, N., and Vale, R. D. (1994) Neuron 12, 1059-1072). To assess the selectivity of kinesin light chain interaction with the heavy chains, we performed immunoprecipitation experiments. The data suggested that the light chains form homodimers with no specificity in their interaction with the two heavy chains. Immunofluorescence and biochemical subfractionation suggested differences in the subcellular localization of the two kinesin light chain gene products. Although both kinesin light chains are distributed throughout the central and peripheral nervous systems, there is enrichment of one in sciatic nerve axons, while the other shows elevated levels in olfactory bulb glomeruli. These results indicate that the mammalian nervous system contains multiple kinesin light chain gene products with potentially distinct functions.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“KLC1 is predominant in neuronal tissues (Rahman et al., 1998).”
From Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis (2015) · cites this paper as Rahman et al., 1998
In the citing paper
“Recent data suggest that while KHC and KLC only form homodimers amongst themselves, there is no specificity in the interaction of KLCs with KHCs (Rahman et al. 1998).”
From Defective kinesin heavy chain behavior in mouse kinesin light chain mutants (1999) · cites this paper as Rahman et al. 1998
In the citing paper
“In mammals, the polypeptide components of kinesin-I are encoded by three KHC genes, KIF5A, KIF5B, and KIF5C (Gudkov et al. 1994; Niclas et al. 1994; Nakagawa et al. 1997; Xia et al. 1998), and three KLC genes, KLC1, KLC2, and KLC3 (Lamerdin et al. 1996; Rahman et al. 1998).”
From Defective kinesin heavy chain behavior in mouse kinesin light chain mutants (1999) · cites this paper as Rahman et al. 1998
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684
A comprehensive set of sequence analysis programs for the VAX
Devereux, J., Haeberli, P. E., & Smithies, O. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.1part1.387 · Cited by 14,222
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
Laemmli, U. K. (1970) · Nature · Cited by 5,673
Altering the Genome by Homologous Recombination
Capecchi, M. R. (1989) · Science · doi:10.1126/science.2660260 · Cited by 2,037
Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility
VALE, R., REESE, T., & SHEETZ, M. (1985) · Cell · doi:10.1016/s0092-8674(85)80099-4 · Cited by 1,993
Guan, K., & Dixon, J. (1991) · Analytical Biochemistry · doi:10.1016/0003-2697(91)90534-z · Cited by 1,735
Sternberger, L. A., & Sternberger, N. H. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.19.6126 · Cited by 1,158
Okada, Y., Yamazaki, H., Sekine‐Aizawa, Y., & Hirokawa, N. (1995) · Cell · doi:10.1016/0092-8674(95)90538-3 · Cited by 679
Tetratrico peptide repeat interactions: to TPR or not to TPR?
Lamb, J. R., Tugendreich, S., & Hieter, P. (1995) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(00)89037-4 · Cited by 626
A novel brain ATPase with properties expected for the fast axonal transport motor
Brady, S. T. (1985) · Nature · doi:10.1038/317073a0 · Cited by 618
KIF1B, a novel microtubule plus end-directed monomeric motor protein for transport of mitochondria
Nangaku, M., Sato-Yoshitake, R., Okada, Y., Noda, Y., Takemura, R., Yamazaki, H., & Hirokawa, N. (1994) · Cell · doi:10.1016/0092-8674(94)90012-4 · Cited by 608
Hirokawa, N., Pfister, K. K., Yorifuji, H., Wagner, M. C., Brady, S. T., & Bloom, G. S. (1989) · Cell · doi:10.1016/0092-8674(89)90691-0 · Cited by 477
Yang, J. T., Laymon, R. A., & Goldstein, L. S. B. (1989) · Cell · doi:10.1016/0092-8674(89)90692-2 · Cited by 395
Kinesin family in murine central nervous system.
Aizawa, H., Sekine, Y., Takemura, R., Zhang, Z., Nangaku, M., & Hirokawa, N. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.119.5.1287 · Cited by 321
Radial extension of macrophage tubular lysosomes supported by kinesin
Hollenbeck, P. J., & Swanson, J. A. (1990) · Nature · doi:10.1038/346864a0 · Cited by 295
Yamazaki, H., Nakata, T., Okada, Y., & Hirokawa, N. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.130.6.1387 · Cited by 293
Bloom, G. S., Wagner, M. C., Pfister, K. K., & Brady, S. T. (1988) · Biochemistry · doi:10.1021/bi00409a043 · Cited by 284
Kinesin associates with anterogradely transported membranous organelles in vivo.
Hirokawa, N., Sato-Yoshitake, R., Kobayashi, N., Pfister, K. K., Bloom, G. S., & Brady, S. T. (1991) · The Journal of Cell Biology · doi:10.1083/jcb.114.2.295 · Cited by 260
Pfister, K. K., Wagner, M. C., Stenoien, D. L., Brady, S. T., & Bloom, G. S. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.108.4.1453 · Cited by 258
Brady, S. T., Pfister, K. K., & Bloom, G. S. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.3.1061 · Cited by 238
KIF3A is a new microtubule-based anterograde motor in the nerve axon.
Kondo, S., Sato-Yoshitake, R., Noda, Y., Aizawa, H., Nakata, T., Matsuura, Y., & Hirokawa, N. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.125.5.1095 · Cited by 237
Evidence that the Head of Kinesin Is Sufficient for Force Generation and Motility in Vitro
Yang, J. T., Saxton, W. M., Stewart, R. J., Raff, E. C., & Goldstein, L. S. B. (1990) · Science · doi:10.1126/science.2142332 · Cited by 225
Kinesin- and Myosin-driven Steps of Vesicle Recruitment for Ca2+-regulated Exocytosis
Bi, G., Morris, R. L., Liao, G., Alderton, J. M., Scholey, J. M., & Steinhardt, R. A. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.138.5.999 · Cited by 211
Suppression of kinesin expression in cultured hippocampal neurons using antisense oligonucleotides.
Ferreira, A., Niclas, J., Vale, R. D., Banker, G. A., & Kosik, K. S. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.117.3.595 · Cited by 190
The quaternary structure of bovine brain kinesin.
Kuznetsov, S., Vaisberg, E. A., Shanina, N. A., Magretova, N. N., Chernyak, V. Y., & Gelfand, V. I. (1988) · The EMBO Journal · doi:10.1002/j.1460-2075.1988.tb02820.x · Cited by 183
WITH APOLOGIES TO SCHEHERAZADE: TAILS OF 1001 KINESIN MOTORS
Goldstein, L. S. B. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.001535 · Cited by 181
Inhibition of kinesin-driven microtubule motility by monoclonal antibodies to kinesin heavy chains.
Ingold, A. L., Cohn, S. A., & Scholey, J. M. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.6.2657 · Cited by 175
Kinesin is responsible for centrifugal movement of pigment granules in melanophores.
Rodionov, V., Gyoeva, F. K., & Gelfand, V. I. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.11.4956 · Cited by 170
Hoshimaru, M., Ray, J., Sah, D. W. Y., & Gage, F. H. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.4.1518 · Cited by 165
Cloning and localization of a conventional kinesin motor expressed exclusively in neurons
Niclas, J., Navone, F., Hom-Booker, N., & Vale, R. D. (1994) · Neuron · doi:10.1016/0896-6273(94)90314-x · Cited by 153
Immunochemical analysis of kinesin light chain function
Stenoien, D. L., & Brady, S. T. (1997) · Molecular Biology of the Cell · doi:10.1091/mbc.8.4.675 · Cited by 143
Gudkov, A. V., Kazarov, A. R., Thimmapaya, R., Axenovich, S. A., Mazo, I. A., & Roninson, I. B. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.9.3744 · Cited by 122
Molecular genetics of kinesin light chains: generation of isoforms by alternative splicing.
Cyr, J. L., Pfister, K. K., Bloom, G. S., Slaughter, C. A., & Brady, S. T. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.22.10114 · Cited by 120
Effects of kinesin mutations on neuronal functions
Gho, M., McDonald, K., Ganetzky, B., & Saxton, W. M. (1992) · Science · doi:10.1126/science.1384131 · Cited by 120
Elluru, R. G., Bloom, G. S., & Brady, S. T. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.1.21 · Cited by 118
The carboxyl-terminal domain of kinesin heavy chain is important for membrane binding
Skoufias, D. A., Cole, D. G., Wedaman, K. P., & Scholey, J. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)42281-2 · Cited by 110
The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain
Gauger, A. K., & Goldstein, L. S. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38698-3 · Cited by 105
Steinberg, G., & Schliwa, M. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.11.1605 · Cited by 102
Characterization of the KIF3C Neural Kinesin-like Motor from Mouse
Yang, Z., & Goldstein, L. S. B. (1998) · Molecular Biology of the Cell · doi:10.1091/mbc.9.2.249 · Cited by 102
Evidence that the stalk of Drosophila kinesin heavy chain is an alpha-helical coiled coil.
de Cuevas, M., Tao, T., & Goldstein, L. S. B. (1992) · The Journal of Cell Biology · doi:10.1083/jcb.116.4.957 · Cited by 100
Characterization of KIFC2, a Neuronal Kinesin Superfamily Member in Mouse
Hanlon, D., Yang, Z., & Goldstein, L. S. B. (1997) · Neuron · doi:10.1016/s0896-6273(00)81244-1 · Cited by 97
Neurofilament protein phosphorylation - where, when and why
Matus, A. I. (1988) · Trends in Neurosciences · doi:10.1016/0166-2236(88)90086-0 · Cited by 88
A Collection of cDNA Clones with Specific Expression Patterns in Mouse Brain
Kato, K. (1990) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.1990.tb00460.x · Cited by 84
Tetratrico peptide repeats are present in the kinesin light chain
Gindhart, J. G., & Goldstein, L. S. B. (1996) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(96)80180-0 · Cited by 76
Amaratunga, A., Morin, P. J., Kosik, K. S., & Fine, R. E. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)85352-8 · Cited by 67
Sequence Analysis of theERCC2Gene Regions in Human, Mouse, and Hamster Reveals Three Linked Genes
Lamerdin, J. E., Stilwagen, S. A., Ramirez, M., Stubbs, L. J., & Carrano, A. V. (1996) · Genomics · doi:10.1006/geno.1996.0303 · Cited by 54
Sequences of Sea Urchin Kinesin Light Chain Isoforms
Wedaman, K. P., Knight, A. E., Kendrick‐Jones, J., & Scholey, J. M. (1993) · Journal of Molecular Biology · doi:10.1006/jmbi.1993.1267 · Cited by 47
Motor activity and mitotic spindle localization of the Drosophila kinesin-like protein KLP61F.
Barton, N. R., Pereira, A., & Goldstein, L. S. B. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.11.1563 · Cited by 45
Beushausen, S. A., Kladakis, A., & Jaffe, H. (1993) · DNA and Cell Biology · doi:10.1089/dna.1993.12.901 · Cited by 36
Kinesin Light Chain Isoforms in Caenorhabditis elegans
Fan, J., & Amos, L. (1994) · Journal of Molecular Biology · doi:10.1006/jmbi.1994.1465 · Cited by 26
Cloning and Genetic Characterization of the Human Kinesin Light-Chain (KLC) Gene
Cabeza-Arvelaiz, Y., SHIH, L.-C. N., Hardman, N., ASSELBERGS, F., Bilbe, G., Schmitz, A., WHITE, B., Siciliano, M. J., & Lachman, L. B. (1993) · DNA and Cell Biology · doi:10.1089/dna.1993.12.881 · Cited by 23
Subcellular localization of the F5 protein to the neuronal membrane‐associated cytoskeleton
Arai, M., & Cohen, J. A. (1994) · Journal of Neuroscience Research · doi:10.1002/jnr.490380313 · Cited by 11
Goedert, M., Marsh, S. G. E., & Carter, N. (1996) · Genomics · doi:10.1006/geno.1996.0102 · Cited by 8
Flagged first, then by citations
Research works printed but not linked: 2
- Hall, 1991, J. Cell Biol., 115, 389
- Bloom, 1994, Protein Profile, 1, 1059
Cited by
Sorted by citations
The Molecular Motor Toolbox for Intracellular Transport
Vale, R. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00111-9 · Cited by 1,982
Microtubule-Based Transport Systems in Neurons: The Roles of Kinesins and Dyneins
Goldstein, L. S. B., & Yang, Z. (2000) · Annual Review of Neuroscience · doi:10.1146/annurev.neuro.23.1.39 · Cited by 563
Kamal, A., Stokin, G. B., Yang, Z., Xia, C.-H., & Goldstein, L. S. B. (2000) · Neuron · doi:10.1016/s0896-6273(00)00124-0 · Cited by 544
Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics
Hirokawa, N., & Noda, Y. (2008) · Physiological Reviews · doi:10.1152/physrev.00023.2007 · Cited by 528
Arl8 and SKIP Act Together to Link Lysosomes to Kinesin-1
Rosa-Ferreira, C., & Munro, S. (2011) · Developmental Cell · doi:10.1016/j.devcel.2011.10.007 · Cited by 360
Metadata
- Authors
- Rahman, A., Friedman, D. S., & Goldstein, L. S. B.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of California, San Francisco (US)
- Corresponding author
- Goldstein, Lawrence S.B.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1998-06
- Volume
- 273
- Issue
- 25
- Pages
- 15395-15403
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1983362841
- Cited by
- 123
- References
- 55
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 8 · 2022: 3 · 2021: 6 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 7 · 2016: 6 · 2015: 5 · 2014: 4 · 2013: 1 · 2012: 4
- FWCI
- 2.16
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- University of California, San Francisco
- Lawrence Livermore National Laboratory
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- kinesin light chain, kinesin heavy chain, gene duplication, neuronal expression, subcellular localization, olfactory bulb glomeruli
- MeSH terms
- Amino Acid Sequence, Animals, Brain Chemistry, Chromosome Mapping, Cloning, Molecular, Humans, Microtubule-Associated Proteins, Microtubules, Molecular Sequence Data, Sciatic Nerve, Kinesins, Sequence Analysis, DNA, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
