Cited by 388Nitric Oxide and Endothelin EffectsMetal-Catalyzed Oxygenation MechanismsHemoglobin structure and function

The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.

Inducible Nitric-oxide Synthase Generates Superoxide from the Reductase Domain

Open access

Xia, Yong, Roman, Linda J., Masters, Bettie Sue S., Zweier, Jay L.·1998·Journal of Biological Chemistry

Open this paper in CoChat — free account Open PDF

Abstract

In the absence of L-arginine, the heme center of the oxygenase domain of neuronal nitric-oxide synthase reduces molecular oxygen to superoxide (O-2). Our recent work has provided evidence that inducible NOS (iNOS) may also catalyze O-2 formation in macrophages. However, there has been a lack of direct evidence of superoxide generation from the purified iNOS, and it was previously hypothesized that significant O-2 production does not occur. Moreover, the mechanism and enzyme site responsible for O-2 generation is unknown. To determine whether iNOS produces O-2 and to identify the mechanism of this process, we performed electron paramagnetic resonance measurements on purified iNOS using the spin trap 5,5-dimethyl-1-pyrroline N-oxide. In the presence of NADPH, prominent O-2 adduct signals were detected from iNOS. These signals were totally abolished by superoxide dismutase but not affected by catalase. High concentrations of L-arginine decreased this O-2 formation, whereas its enantiomer D-arginine did not. Pre-incubation of iNOS with the flavoprotein inhibitor diphenyleneiodonium totally blocked these O-2 signals. Conversely, pretreatment of the enzyme with the heme blocker cyanide had no effect on O-2 generation. Furthermore, strong O-2 generation was directly detected from the isolated iNOS reductase domain. Together, these data demonstrate that iNOS does generate O-2, and this mainly occurs at the flavin-binding sites of the reductase domain.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1074/jbc.273.35.22635

Cites

29 of 29 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Nitric oxide: physiology, pathophysiology, and pharmacology.
Moncada, S., Palmer, R. M., & Higgs, E. A. (1991) · Pharmacological Reviews · doi:10.1016/s0031-6997(25)06663-3 · Cited by 15,525
Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide
Beckman, J. S., Beckman, T. W., Chen, J., Marshall, P. A., & Freeman, B. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.4.1620 · Cited by 7,029
Nitric oxide synthases: Roles, tolls, and controls
Nathan, C., & Xie, Q. W. (1994) · Cell · doi:10.1016/0092-8674(94)90266-6 · Cited by 2,920
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase
Bredt, D. S., Hwang, P. M., Glatt, C. E., Lowenstein, C. J., Reed, R. R., & Snyder, S. H. (1991) · Nature · doi:10.1038/351714a0 · Cited by 2,354
Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum.
Bredt, D. S., & Snyder, S. H. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.22.9030 · Cited by 1,849
Nitric oxide synthase structure and mechanism
Marletta, M. A. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31375-9 · Cited by 1,338
Nitric Oxide Synthases: Properties and Catalytic Mechanism
Griffith, O. W., & Stuehr, D. J. (1995) · Annual Review of Physiology · doi:10.1146/annurev.ph.57.030195.003423 · Cited by 1,311
Generation of superoxide by purified brain nitric oxide synthase
Pou, S., Pou, W. S., Bredt, D. S., Snyder, S. H., & Rosen, G. M. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)35745-4 · Cited by 750
Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury.
Xia, Y., Dawson, V. L., Dawson, T. M., Snyder, S. H., & Zweier, J. L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.13.6770 · Cited by 717
Self-citation
Superoxide and peroxynitrite generation from inducible nitric oxide synthase in macrophages
Xia, Y., & Zweier, J. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6954 · Cited by 691
Self-citation

Flagged first, then by citations

Show all 29 references

Research works printed but not linked: 2

Moncada, 1991, Pharmacol. Rev., 43, 109
Finkelstein, 1982, Mol. Pharmacol., 21, 262

Sorted by citations

Nitric oxide synthases: structure, function and inhibition
ALDERTON, W. K., COOPER, C. E., & KNOWLES, R. G. (2001) · Biochemical Journal · doi:10.1042/bj3570593 · Cited by 4,110
The metabolic signature of macrophage responses
Viola, A., Munari, F., Sánchez-Rodríguez, R., Scolaro, T., & Castegna, A. (2019) · Frontiers in Immunology · doi:10.3389/fimmu.2019.01462 · Cited by 2,021
Reactive Oxygen Species in Metabolic and Inflammatory Signaling
Forrester, S. J., Kikuchi, D. S., Hernandes, M. S., Xu, Q., & Griendling, K. K. (2018) · Circulation Research · doi:10.1161/circresaha.117.311401 · Cited by 2,081
Regulation of immune responses by L-arginine metabolism
Bronte, V., & Zanovello, P. (2005) · Nature Reviews Immunology · doi:10.1038/nri1668 · Cited by 1,775
Oxygen, oxidative stress, hypoxia, and heart failure
Giordano, F. J. (2005) · Journal of Clinical Investigation · doi:10.1172/jci24408 · Cited by 1,515
Reactive oxygen and reactive nitrogen intermediates in innate and specific immunity
Bogdan, C., Röllinghoff, M., & Diefenbach, A. (2000) · Current Opinion in Immunology · doi:10.1016/s0952-7915(99)00052-7 · Cited by 952
Myeloid suppressor cells in cancer: Recruitment, phenotype, properties, and mechanisms of immune suppression
Serafini, P., Borrello, I., & Bronte, V. (2005) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2005.07.005 · Cited by 775
Enzymatic function of nitric oxide synthases
Andrew, P. (1999) · Cardiovascular Research · doi:10.1016/s0008-6363(99)00115-7 · Cited by 776
The role of oxidants and free radicals in reperfusion injury
Zweier, J. L., & Talukder, M. F. (2006) · Cardiovascular Research · doi:10.1016/j.cardiores.2006.02.025 · Cited by 743
Self-citation
Chemokine nitration prevents intratumoral infiltration of antigen-specific T cells
Molon, B., Ugel, S., Pozzo, F. D., Soldani, C., Zilio, S., Avella, D., Palma, A. D., Mauri, P., Monegal, A., Rescigno, M., et al. (2011) · The Journal of Experimental Medicine · doi:10.1084/jem.20101956 · Cited by 685
Inflammation, Oxidative Stress, and Repair Capacity of the Vascular Endothelium in Obstructive Sleep Apnea
Jelić, S., Padeletti, M., Kawut, S. M., Higgins, C., Canfield, S. M., Onat, D., Colombo, P. C., Basner, R. C., Factor, P. H., & LeJemtel, T. H. (2008) · Circulation · doi:10.1161/circulationaha.107.741512 · Cited by 631
Tumor‐induced tolerance and immune suppression by myeloid derived suppressor cells
Marigo, I., Dolcetti, L., Serafini, P., Zanovello, P., & Bronte, V. (2008) · Immunological Reviews · doi:10.1111/j.1600-065x.2008.00602.x · Cited by 626
L-arginine metabolism in myeloid cells controls T-lymphocyte functions
Bronte, V., Serafini, P., Mazzoni, A., Segal, D. M., & Zanovello, P. (2003) · Trends in Immunology · doi:10.1016/s1471-4906(03)00132-7 · Cited by 599
Nitric oxide: comparative synthesis and signaling in animal and plant cells
Wendehenne, D., Pugin, A., Klessig, D. F., & Durner, J. (2001) · Trends in Plant Science · doi:10.1016/s1360-1385(01)01893-3 · Cited by 571
Nitric oxide, cell bioenergetics and neurodegeneration
Moncada, S., & Bolaños, J. P. (2006) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2006.03988.x · Cited by 555
The Role of Oxidative Stress in Atherosclerosis
Batty, M., Bennett, M. R., & Yu, E. (2022) · Cells · doi:10.3390/cells11233843 · Cited by 586
Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation
Tejero, J., Shiva, S., & Gladwin, M. T. (2019) · Physiological Reviews · doi:10.1152/physrev.00036.2017 · Cited by 541
Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells
Corpas, F. J., Barroso, J. B., & del Río, L. A. (2001) · Trends in Plant Science · doi:10.1016/s1360-1385(01)01898-2 · Cited by 531
IL-4-Induced Arginase 1 Suppresses Alloreactive T Cells in Tumor-Bearing Mice
Bronte, V., Serafini, P., Santo, C. D., Marigo, I., Tosello, V., Mazzoni, A., Segal, D. M., Staib, C., Löwel, M., Sutter, G., et al. (2003) · The Journal of Immunology · doi:10.4049/jimmunol.170.1.270 · Cited by 526
Cellular regulation of endothelial nitric oxide synthase
Govers, R., & Rabelink, T. J. (2001) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.2001.280.2.f193 · Cited by 476
Bone marrow myeloid-derived suppressor cells (MDSCs) inhibit graft-versus-host disease (GVHD) via an arginase-1–dependent mechanism that is up-regulated by interleukin-13
Highfill, S. L., Rodrı́guez, P. C., Zhou, Q., Goetz, C. A., Koehn, B. H., Veenstra, R. G., Taylor, P. A., Panoskaltsis‐Mortari, A., Serody, J. S., Munn, D. H., et al. (2010) · Blood · doi:10.1182/blood-2010-06-287839 · Cited by 465
Oxygen Reduction by Nitric-oxide Synthases
Stuehr, D. J., Pou, S., & Rosen, G. M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r100011200 · Cited by 409
Boosting antitumor responses of T lymphocytes infiltrating human prostate cancers
Bronte, V., Kasic, T., Gri, G., Gallana, K., Borsellino, G., Marigo, I., Battistini, L., Iafrate, M., Prayer‐Galetti, T., Pagano, F. C., et al. (2005) · The Journal of Experimental Medicine · doi:10.1084/jem.20042028 · Cited by 408
The cellular and molecular origin of reactive oxygen species generation during myocardial ischemia and reperfusion
Raedschelders, K., Ansley, D. M., & Chen, D. D. Y. (2011) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.11.004 · Cited by 393
Oxidative stress and anti-oxidative mobilization in burn injury
Parihar, A., Parihar, M. S., Milner, S., & Bhat, S. G. (2007) · Burns · doi:10.1016/j.burns.2007.04.009 · Cited by 382
The NOX toolbox: validating the role of NADPH oxidases in physiology and disease
Altenhöfer, S., Kleikers, P. W. M., Radermacher, K. A., Scheurer, P., Rob Hermans, J. J., Schiffers, P., Ho, H., Wingler, K., & Schmidt, H. H. H. W. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1010-9 · Cited by 371
Arginine-dependent immune responses
Líndez, A.-A. M. I., & Reith, W. (2021) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-021-03828-4 · Cited by 384
A mitochondrial superoxide theory for oxidative stress diseases and aging
Indo, H. P., Yen, H., Nakanishi, I., Matsumoto, K., Tamura, M., Nagano, Y. N., Matsui, H., Gusev, O., Cornette, R., Okuda, T., et al. (2014) · Journal of Clinical Biochemistry and Nutrition · doi:10.3164/jcbn.14-42 · Cited by 349
New Molecular Mechanisms on the Activation of TRPM2 Channels by Oxidative Stress and ADP-Ribose
Nazıroğlu, M. (2007) · Neurochemical Research · doi:10.1007/s11064-007-9386-x · Cited by 325
Nitric oxide biosynthesis, nitric oxide synthase inhibitors and arginase competition for L-arginine utilization
Boucher, J., Moali, C., & Tenu, J. (1999) · Cellular and Molecular Life Sciences · doi:10.1007/s000180050352 · Cited by 314
Control of immune response by amino acid metabolism
Grohmann, U., & Bronte, V. (2010) · Immunological Reviews · doi:10.1111/j.1600-065x.2010.00915.x · Cited by 321
eNOS Uncoupling in Cardiovascular Diseases - the Role of Oxidative Stress and Inflammation
Karbach, S., Wenzel, P., Waisman, A., Munzel, T., & Daiber, A. (2014) · Current Pharmaceutical Design · doi:10.2174/13816128113196660748 · Cited by 307
Diabetes-Induced Reactive Oxygen Species: Mechanism of Their Generation and Role in Renal Injury
Fakhruddin, S., Alanazi, W. A., & Jackson, K. E. (2017) · Journal of Diabetes Research · doi:10.1155/2017/8379327 · Cited by 300
Pathways Mediating the Expansion and Immunosuppressive Activity of Myeloid-Derived Suppressor Cells and Their Relevance to Cancer Therapy
Talmadge, J. E. (2007) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-0182 · Cited by 286
Oxidative Stress in Human Abdominal Aortic Aneurysms
Miller, F. J., Sharp, W. J., Fang, X., Oberley, L. W., Oberley, T. D., & Weintraub, N. L. (2002) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000013778.72404.30 · Cited by 277
The heme oxygenase pathway and its interaction with nitric oxide in the control of cellular homeostasis
Foresti, R., & Motterlini, R. (1999) · Free Radical Research · doi:10.1080/10715769900301031 · Cited by 271
Oxidative and Antioxidative Potential of Brain Microglial Cells
Dringen, R. (2005) · Antioxidants and Redox Signaling · doi:10.1089/ars.2005.7.1223 · Cited by 270
Metalloporphyrin class of therapeutic catalytic antioxidants
Patel, M., & Day, B. J. (1999) · Trends in Pharmacological Sciences · doi:10.1016/s0165-6147(99)01336-x · Cited by 253
Genetic Deficiency of Inducible Nitric Oxide Synthase Reduces Atherosclerosis and Lowers Plasma Lipid Peroxides in Apolipoprotein E–Knockout Mice
Kuhlencordt, P. J., Chen, J., Han, F., Astern, J., & Huang, P. L. (2001) · Circulation · doi:10.1161/01.cir.103.25.3099 · Cited by 252
Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function
Jackson, M. J. (2002) · Molecular Aspects of Medicine · doi:10.1016/s0098-2997(02)00018-3 · Cited by 238
Differential Effects of Green Tea–Derived Catechin on Developing Versus Established Atherosclerosis in Apolipoprotein E–Null Mice
Chyu, K., Babbidge, S. M., Zhao, X., Dandillaya, R., Rietveld, A. G., Yano, J., Dimayuga, P. C., Cercek, B., & Shah, P. K. (2004) · Circulation · doi:10.1161/01.cir.0000128034.70732.c2 · Cited by 224
Detection of Reactive Oxygen and Nitrogen Species by EPR Spin Trapping
Villamena, F. A., & Zweier, J. L. (2004) · Antioxidants and Redox Signaling · doi:10.1089/152308604773934387 · Cited by 220
Self-citation
Immunology and Oxidative Stress in Multiple Sclerosis: Clinical and Basic Approach
Ortíz, G. G., Pacheco-Moisés, F. P., Bitzer-Quintero, O. K., Anguiano, A. C. R., Flores-Alvarado, L. J., Ramírez-Ramírez, V., Macías-Islas, M. Á., & Torres-Sánchez, E. D. (2013) · Clinical and Developmental Immunology · doi:10.1155/2013/708659 · Cited by 221
Immune cell - produced ROS and their impact on tumor growth and metastasis
Kennel, K. B., & Greten, F. R. (2021) · Redox Biology · doi:10.1016/j.redox.2021.101891 · Cited by 209
A20 Inhibits Cytokine-Induced Apoptosis and Nuclear Factor κB–Dependent Gene Activation in Islets
Grey, S. T., Arvelo, M. B., Hasenkamp, W. M., Bach, F. H., & Ferran, C. J. (1999) · The Journal of Experimental Medicine · doi:10.1084/jem.190.8.1135 · Cited by 208
Classical Inhibitors of NOX NAD(P)H Oxidases Are Not Specific
Aldieri, E., Riganti, C., Polimeni, M., Gazzano, E., Lussiana, C., Campia, I., & Ghigo, D. (2008) · Current Drug Metabolism · doi:10.2174/138920008786049285 · Cited by 201
Myocardial ischemia results in tetrahydrobiopterin (BH 4 ) oxidation with impaired endothelial function ameliorated by BH 4
Dumitrescu, C., Biondi, R., Xia, Y., Cardounel, A. J., Druhan, L. J., Ambrosio, G., & Zweier, J. L. (2007) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0702986104 · Cited by 192
Self-citation
Mechanism of Superoxide Generation by Neuronal Nitric-oxide Synthase
Pou, S., Keaton, L., Surichamorn, W., & Rosen, G. M. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.14.9573 · Cited by 188
Electron transfer by diflavin reductases
Murataliev, M. B., Feyereisen, R., & Walker, F. A. (2003) · Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics · doi:10.1016/j.bbapap.2003.10.003 · Cited by 185
Phosphorylation of Endothelial Nitric-oxide Synthase Regulates Superoxide Generation from the Enzyme
Chen, C., Druhan, L. J., Varadharaj, S., Chen, Y., & Zweier, J. L. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m802269200 · Cited by 173
Self-citation
Carcinogenesis mechanisms of Fusobacterium nucleatum
Gholizadeh, P., Eslami, H., & Kafil, H. S. (2017) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2017.02.102 · Cited by 175
Photic Signaling by Cryptochrome in the Drosophila Circadian System
Lin, F.-J., Song, W., Meyer-Bernstein, E. L., Naidoo, N. N., & Sehgal, A. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.21.7287-7294.2001 · Cited by 169
Effects of nitric oxide and nitric oxide‐derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis
Goodwin, D. C., Landino, L. M., & Marnett, L. J. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.10.1121 · Cited by 168
Peroxynitrite Protects Neurons against Nitric Oxide-mediated Apoptosis
Garcı́a-Nogales, P., Almeida, Á., & Bolaños, J. P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m206835200 · Cited by 164
Regulation of iNOS function and cellular redox state by macrophage Gch1 reveals specific requirements for tetrahydrobiopterin in NRF2 activation
McNeill, E., Crabtree, M. J., Sahgal, N., Patel, J., Chuaiphichai, S., Iqbal, A. J., Hale, A., Greaves, D. R., & Channon, K. M. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.10.575 · Cited by 150
Macrophage-Mediated Innate Host Defense Against Protozoan Parasites
Stafford, J. L., Neumann, N. F., & Belosevic, M. (2002) · Critical Reviews in Microbiology · doi:10.1080/1040-840291046731 · Cited by 163
Role of neuronal and endothelial nitric oxide synthase in nitric oxide generation in the brain following cerebral ischemia
Wei, G., Dawson, V. L., & Zweier, J. L. (1999) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/s0925-4439(99)00051-4 · Cited by 157
Self-citation
Glucose‐6‐phosphate dehydrogenase deficiency promotes endothelial oxidant stress and decreases endothelial nitric oxide bioavailability
Leopold, J. A., Cap, A., Scribner, A. W., Stanton, R. C., & Loscalzo, J. (2001) · The FASEB Journal · doi:10.1096/fj.00-0893fje · Cited by 153
Arginase: a key enzyme in the pathophysiology of allergic asthma opening novel therapeutic perspectives
Maarsingh, H., Zaagsma, J., & Meurs, H. (2009) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.2009.00374.x · Cited by 151
Inducible Nitric Oxide Synthase Deficiency Protects the Heart From Systolic Overload–Induced Ventricular Hypertrophy and Congestive Heart Failure
Zhang, P., Xu, X., Hu, X., van Deel, E. D., Zhu, G., & Chen, Y. (2007) · Circulation Research · doi:10.1161/01.res.0000264081.78659.45 · Cited by 152
Inducible Nitric Oxide Synthase Mediates Delayed Myocardial Protection Induced by Activation of Adenosine A 1 Receptors
Zhao, T., Xi, L., Chelliah, J. C., Levasseur, J. E., & Kukreja, R. C. (2000) · Circulation · doi:10.1161/01.cir.102.8.902 · Cited by 152
Mechanism of Free-Radical Generation by Nitric Oxide Synthase
Rosen, G. M., Tsai, P., & Pou, S. (2002) · Chemical Reviews · doi:10.1021/cr010187s · Cited by 153
Endothelial Function in Obstructive Sleep Apnea
Atkeson, A. D., Yeh, S. Y., Malhotra, A., & Jelić, S. (2009) · Progress in Cardiovascular Diseases · doi:10.1016/j.pcad.2008.08.002 · Cited by 154
Arginase and pulmonary diseases
Maarsingh, H., Pera, T., & Meurs, H. (2008) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/s00210-008-0286-7 · Cited by 146
PGE2-Driven Induction and Maintenance of Cancer-Associated Myeloid-Derived Suppressor Cells
Obermajer, N., Wong, J. L., Edwards, R. P., Odunsi, K. O., Moysich, K. B., & Kaliński, P. (2012) · Immunological Investigations · doi:10.3109/08820139.2012.695417 · Cited by 146
Asymmetric Dimethylarginine Induces Oxidative and Nitrosative Stress in Murine Lung Epithelial Cells
Wells, S. M., & Holian, A. (2006) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/rcmb.2006-0302sm · Cited by 144
Tetrahydrobiopterin and Nitric Oxide: Mechanistic and Pharmacological Aspects
Werner, E. R., Gorren, A. C. F., Heller, R., Werner‐Felmayer, G., & Mayer, B. (2003) · Experimental Biology and Medicine · doi:10.1177/153537020322801108 · Cited by 145
Mitochondrial Reactive Oxygen Species and Risk of Atherosclerosis
Hulsmans, M., Dooren, E. V., & Holvoet, P. (2012) · Current Atherosclerosis Reports · doi:10.1007/s11883-012-0237-0 · Cited by 132
Nitric-oxide synthase: A cytochrome P450 family foster child
Gorren, A. C. F., & Mayer, B. (2007) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2006.08.019 · Cited by 129
Superoxide Generation from Mitochondrial NADH Dehydrogenase Induces Self-inactivation with Specific Protein Radical Formation
Chen, Y., Chen, C., Zhang, L., Green‐Church, K. B., & Zweier, J. L. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m503936200 · Cited by 130
Self-citation
HMG-CoA Reductase Inhibitor Increases GTP Cyclohydrolase I mRNA and Tetrahydrobiopterin in Vascular Endothelial Cells
Hattori, Y., Nakanishi, N., Akimoto, K., Yoshida, M., & Kasai, K. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000054659.72231.a1 · Cited by 127
Atherosclerosis: from lesion formation to plaque activation and endothelial dysfunction
Keaney, J. F. (2000) · Molecular Aspects of Medicine · doi:10.1016/s0098-2997(00)00005-4 · Cited by 124
Reactive oxygen species as mediators of organ dysfunction caused by sepsis, acute respiratory distress syndrome, or hemorrhagic shock: potential benefits of resuscitation with Ringerʼs ethyl pyruvate solution
Fink, M. P. (2002) · Current Opinion in Clinical Nutrition & Metabolic Care · doi:10.1097/00075197-200203000-00009 · Cited by 126
A Neurovascular Perspective for Long-Term Changes After Brain Trauma
Pop, V., & Badaut, J. (2011) · Translational Stroke Research · doi:10.1007/s12975-011-0126-9 · Cited by 125
Vascular Generation of Tumor Necrosis Factor-α Reduces Nitric Oxide Availability in Small Arteries From Visceral Fat of Obese Patients
Virdis, A., Santini, F., Colucci, R., Duranti, E., Salvetti, G., Rugani, I., Segnani, C., Anselmino, M., Bernardini, N., Blandizzi, C., et al. (2011) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2011.01.050 · Cited by 119
Endothelial Cell Activation in Patients With Decompensated Heart Failure
Colombo, P. C., Banchs, J. E., Celaj, S., Talreja, A., Lachmann, J. S., Malla, S., Dubois, N. B., Ashton, A. W., Latif, F., Jorde, U. P., et al. (2004) · Circulation · doi:10.1161/01.cir.0000151611.89232.3b · Cited by 122
Glucose deprivation causes oxidative stress and stimulates aggresome formation and autophagy in cultured cardiac myocytes
Marambio, P., Toro, B., Sanhueza, C., Troncoso, R., Parra, V., Verdejo, H., García, L., Quiroga, C., Munafó, D. B., Díaz‐Elizondo, J., et al. (2010) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2010.02.002 · Cited by 122
Cytokine-induced apoptosis and necrosis are preceded by disruption of the mitochondrial membrane potential (Δψm) in pancreatic RINm5F cells: prevention by Bcl-2
Barbu, A. R., Welsh, N., & Saldeen, J. (2002) · Molecular and Cellular Endocrinology · doi:10.1016/s0303-7207(02)00009-6 · Cited by 121
Overexpression of Inducible Nitric Oxide Synthase in Rostral Ventrolateral Medulla Causes Hypertension and Sympathoexcitation via an Increase in Oxidative Stress
Kimura, Y., Hirooka, Y., Sagara, Y., Ito, K., Kishi, T., Shimokawa, H., Takeshita, A., & Sunagawa, K. (2005) · Circulation Research · doi:10.1161/01.res.0000152965.75127.9d · Cited by 121
Nitric Oxide Regulates Human Sperm Capacitation and Protein-Tyrosine Phosphorylation In Vitro1
Herrero, M. B., de Lamirande, E., & Gagnon, C. (1999) · Biology of Reproduction · doi:10.1095/biolreprod61.3.575 · Cited by 121
Heat Shock Protein 90 as an Endogenous Protein Enhancer of Inducible Nitric-oxide Synthase
Yoshida, M., & Xia, Y. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m305214200 · Cited by 119
Self-citation
Mechanism of nitric oxide synthase regulation: Electron transfer and interdomain interactions
Feng, C. (2011) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2011.10.011 · Cited by 128
Lactate dehydrogenases amplify reactive oxygen species in cancer cells in response to oxidative stimuli
Wu, H., Wang, Y., Ying, M., Jin, C., Li, J., & Hu, X. (2021) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-021-00595-3 · Cited by 122
Mitochondria and reactive oxygen and nitrogen species in neurological disorders and stroke: Therapeutic implications☆
Bolaños, J. P., Moro, M. Á., Lizasoaín, I., & Almeida, Á. (2009) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2009.05.009 · Cited by 120
Myeloid-derived suppressor cell role in tumor-related inflammation
Dolcetti, L., Marigo, I., Mantelli, B., Peranzoni, E., Zanovello, P., & Bronte, V. (2008) · Cancer Letters · doi:10.1016/j.canlet.2008.03.012 · Cited by 122
Reactive oxygen and nitrogen species regulate inducible nitric oxide synthase function shifting the balance of nitric oxide and superoxide production
Sun, J., Druhan, L. J., & Zweier, J. L. (2009) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2009.11.019 · Cited by 122
Self-citation
The Biological Chemistry of Peroxynitrite
Radí, R., Denicola, A., Álvarez, B., Ferrer‐Sueta, G., & Rubbo, H. (2000) · Elsevier eBooks · doi:10.1016/b978-012370420-7/50005-8 · Cited by 115
Role of reactive oxygen and nitrogen species in acute respiratory distress syndrome
Fink, M. P. (2002) · Current Opinion in Critical Care · doi:10.1097/00075198-200202000-00002 · Cited by 113
Arginase Inhibition Protects against Allergen-induced Airway Obstruction, Hyperresponsiveness, and Inflammation
Maarsingh, H., Zuidhof, A. B., Bos, I. S. T., van Duin, M., Boucher, J., Zaagsma, J., & Meurs, H. (2008) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.200710-1588oc · Cited by 107
Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species
Shi, H., Timmins, G. S., Monske, M., Burdick, A. D., Kalyanaraman, B., Liu, Y., Jean-Louis, C., Burchiel, S. W., & Liu, K. J. (2005) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2005.02.028 · Cited by 103
Decreased Neointimal Thickening After Arterial Wall Injury in Inducible Nitric Oxide Synthase Knockout Mice
Chyu, K., Dimayuga, P. C., Zhu, J., Nilsson, J., Kaul, S., Shah, P. K., & Cercek, B. (1999) · Circulation Research · doi:10.1161/01.res.85.12.1192 · Cited by 103
Mitochondrial KATPChannel as an End Effector of Cardioprotection During Late Preconditioning: Triggering Role of Nitric Oxide
Wang, Y., Kudo, M., Xu, M., Ayub, A., & Ashraf, M. (2001) · Journal of Molecular and Cellular Cardiology · doi:10.1006/jmcc.2001.1468 · Cited by 99
Nitric Oxide Synthase in Atherosclerosis and Vascular Injury
Channon, K. M., Qian, H., & George, S. E. (2000) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.20.8.1873 · Cited by 99
RETRACTED: Ginger extract ameliorates bisphenol A (BPA)-induced disruption in thyroid hormones synthesis and metabolism: Involvement of Nrf-2/HO-1 pathway
Mohammed, E. T., Hashem, K. S., Ahmed, A. E., Aly, M. T., Aleya, L., & Abdel‐Daim, M. M. (2019) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2019.134664 · Cited by 99
Retracted
Arginine, nitric oxide, carbon monoxide, and endothelial function in severe malaria
Weinberg, J. B., Lopansri, B. K., Mwaikambo, E. D., & Granger, D. L. (2008) · Current Opinion in Infectious Diseases · doi:10.1097/qco.0b013e32830ef5cf · Cited by 99
Nitric oxide donor drugs: current status and future trends
Webb, D. J., & Megson, I. L. (2002) · Expert Opinion on Investigational Drugs · doi:10.1517/13543784.11.5.587 · Cited by 95
Relative deficiency of nitric oxide-dependent vasodilation in salt-hypertensive Dahl rats: the possible role of superoxide anions
Zicha, J., Dobešová, Z., & Kuneš, J. (2001) · Journal of Hypertension · doi:10.1097/00004872-200102000-00011 · Cited by 95
The Role of Tetrahydrobiopterin in the Regulation of Neuronal Nitric-oxide Synthase-generated Superoxide
Rosen, G. M., Tsai, P., Weaver, J. M. R., Porasuphatana, S., Roman, L. J., Starkov, A. A., Fiskum, G. M., & Pou, S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200853200 · Cited by 94
Self-citation
Myeloid suppressor cells regulate the adaptive immune response to cancer
Frey, A. B. (2006) · Journal of Clinical Investigation · doi:10.1172/jci29906 · Cited by 93
Regulation of Lymphocytes by Nitric Oxide
Bogdan, C. (2010) · Methods in molecular biology · doi:10.1007/978-1-60761-869-0_24 · Cited by 95

Authors: Xia, Y., Roman, L. J., Masters, B. S. S., & Zweier, J. L.
Affiliations: Johns Hopkins University (US)
The University of Texas at San Antonio Health Science Center (US)
Corresponding author: Zweier, Jay L.
ORCID: Xia, Yong
Zweier, Jay L.
Venue: Journal of Biological Chemistry
Publisher: Elsevier BV
Published: 1998-08
Volume: 273
Issue: 35
Pages: 22635-22639
ISSN: 0021-9258 (ISSN-L)
DOI: 10.1074/jbc.273.35.22635
OpenAlex: W2064619021
Cited by: 388
References: 29
Citations by year: 2026: 1 · 2025: 3 · 2024: 2 · 2023: 7 · 2022: 2 · 2021: 8 · 2020: 7 · 2019: 5 · 2018: 9 · 2017: 10 · 2016: 9 · 2015: 6 · 2014: 12 · 2013: 18 · 2012: 14
FWCI: 10.43
Type: journal article
Language: en
Open access: hybrid
doi.org
Licence: cc-by-nc-nd
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
creativecommons.org/licenses/by/4.0/ (vor, 8109 d)
Locations: Journal of Biological Chemistry (publishedVersion, cc-by-nc-nd)
PubMed (publishedVersion)
Funders: Welch Foundation (AQ-1192)
American Heart Association
National Institutes of Health (HL38324)
National Heart, Lung, and Blood Institute (HL-38324, HL52315, P50HL052315)
National Institute of General Medical Sciences (R01GM052419, GM-52419)
Topics: Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
Keywords: inducible nitric oxide synthase, superoxide production, oxygenase domain, reductase domain, flavin binding site, L-arginine, diphenyleneiodonium, flavoprotein, macrophages, spin trapping, NADPH, superoxide dismutase
MeSH terms: Animals, Catalysis, Cyclic N-Oxides, Electron Spin Resonance Spectroscopy, Oxidoreductases, Oxygenases, Recombinant Proteins, Spin Labels, Superoxides, Nitric Oxide Synthase, Mice, Nitric Oxide Synthase Type II

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Inducible Nitric-oxide Synthase Generates Superoxide from the Reductase Domain

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks