Cited by 129Metal-Catalyzed Oxygenation MechanismsNitric Oxide and Endothelin EffectsHemoglobin structure and function

Nitric-oxide synthase: A cytochrome P450 family foster child

Gorren, Antonius C.F., Mayer, Bernd·2007·Biochimica et Biophysica Acta (BBA) - General Subjects

Abstract

Nitric-oxide synthase (NOS), the enzyme responsible for mammalian NO generation, is no cytochrome P450, but there are striking similarities between both enzymes. First and foremost, both are heme-thiolate proteins, employing the same prosthetic group to perform similar chemistry. Moreover, they share the same redox partner, a diflavoprotein reductase, which in the case of NOS is incorporated with the oxygenase in one polypeptide chain. There are, however, also conspicuous differences, such as the presence in NOS of the additional cofactor tetrahydrobiopterin, which is applied as an auxiliary electron donor to prevent decay of the oxyferrous complex to ferric heme and superoxide. In this review similarities and differences between NOS and cytochrome P450 are analyzed in an attempt to explain why NOS requires BH4 and why NO synthesis is not catalyzed by a member of the cytochrome P450 family.

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Crossref · 10.1016/j.bbagen.2006.08.019

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Authors
Gorren, A. C. F., & Mayer, B.
Affiliations
University of Graz (AT)
Corresponding author
Gorren, Antonius C.F.
Venue
Biochimica et Biophysica Acta (BBA) - General Subjects
Publisher
Elsevier BV
Published
2007-03
Volume
1770
Issue
3
Pages
432-445
ISSN
0304-4165 (ISSN-L)
OpenAlex
W2044259132
Cited by
129
References
134
Citations by year
2026: 2 · 2025: 3 · 2024: 5 · 2023: 1 · 2022: 6 · 2021: 6 · 2020: 9 · 2019: 5 · 2018: 6 · 2017: 4 · 2016: 6 · 2015: 6 · 2014: 4 · 2013: 13 · 2012: 4
FWCI
0.82
Type
journal article
Language
en
Open access
closed
Locations
  • Biochimica et Biophysica Acta (BBA) - General Subjects (publishedVersion)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund
Topics
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Hemoglobin structure and function
Keywords
nitric oxide synthase, cytochrome P450, tetrahydrobiopterin, flavoprotein reductase, oxygenase, superoxide, electron transfer, enzyme evolution, nitric oxide production
MeSH terms
Animals, Bacterial Proteins, Biological Transport, Cytochrome P-450 Enzyme System, Humans, Nitric Oxide, Oxidation-Reduction, Protons, Nitric Oxide Synthase

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