Cited by 166Heat shock proteins researchProtein Structure and DynamicsGenomics and Chromatin Dynamics

Identification of Conserved Residues Required for the Binding of a Tetratricopeptide Repeat Domain to Heat Shock Protein 90

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Russell, Lance C., Whitt, Sherry R., Chen, Mei-Shya, Chinkers, Michael·1999·Journal of Biological Chemistry

Abstract

The sequential binding of heat shock protein 90 (hsp90) to a series of tetratricopeptide repeat (TPR) proteins is critical to its function as a molecular chaperone. We have used site-directed mutagenesis to clarify the structural basis for the binding of hsp90 to the TPR domain of phosphoprotein phosphatase 5 (PP5). This TPR domain was chosen for study because its three-dimensional structure is known. We examined co-immunoprecipitation of hsp90 with wild type and mutant TPR constructs from transfected cells. Only mutations located on one face of the TPR domain affected hsp90 binding. This allowed the identification of a binding groove. Three basic residues that are highly conserved in hsp90-binding TPR proteins extend prominently into this groove. Lys-97 and Arg-101 were absolutely required for hsp90 binding, while mutation of Arg-74 diminished, but did not abrogate, hsp90 binding. Mutation of Lys-32, another conserved basic residue in the binding groove, also blocked hsp90 binding. The TPR domain of PP5 bound specifically to a 12-kDa C-terminal fragment of hsp90. This binding was reduced by mutation of acidic residues in the hsp90 fragment. These data suggest conservation, among hsp90-binding TPR proteins, of a binding groove containing basic residues that interact with acidic residues near the C terminus of hsp90.

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  1. [1]1. Parsell, D.A. ∙ Lindquist, S. Annu. Rev. Genet. 1993; 27:437-496 Crossref Scopus (1954) PubMed Google Scholar
  2. [2]2. Pratt, W.B. Proc. Soc. Exp. Biol. Med. 1998; 217:420-434 Crossref Scopus (423) PubMed Google Scholar
  3. [3]3. Pratt, W.B. ∙ Toft, D.O. Endocr. Rev. 1997; 18:306-360 Crossref Scopus (1573) PubMed Google Scholar
  4. [4]4. Chen, C.-F. ∙ Chen, Y. ∙ Dai, K. ... Mol. Cell. Biol. 1996; 16:4691-4699 Crossref Scopus (160) PubMed Google Scholar
  5. [5]5. Whitesell, L. ∙ Sutphin, P.D. ∙ Pulcini, E.J. ... Mol. Cell. Biol. 1998; 18:1517-1524 Crossref Scopus (195) PubMed Google Scholar
  6. [6]6. Garcı́a-Cardeña, G. ∙ Fan, R. ∙ Shah, V. ... Nature. 1998; 392:821-824 Crossref Scopus (886) PubMed Google Scholar
  7. [7]7. Loo, M.A. ∙ Jensen, T.J. ∙ Cui, L. ... EMBO J. 1998; 17:6879-6887 Crossref Scopus (307) PubMed Google Scholar
  8. [8]8. Rutherford, S.L. ∙ Lindquist, S. Nature. 1998; 396:336-342 Crossref Scopus (1779) PubMed Google Scholar
  9. [9]9. Smith, D. ∙ Whitesell, L. ∙ Katsanis, E. Pharmacol. Rev. 1998; 50:493-513 Full Text Full Text (PDF) PubMed Google Scholar
  10. [10]10. Scheibel, T. ∙ Buchner, J. Biochem. Pharmacol. 1998; 56:675-682 Crossref Scopus (165) PubMed Google Scholar
  11. [11]11. Lamb, J.R. ∙ Tugendreich, S. ∙ Hieter, P. Trends Biochem. Sci. 1995; 20:257-259 Full Text (PDF) Scopus (562) PubMed Google Scholar
  12. [12]12. Frydman, J. ∙ Höhfeld, J. Trends Biochem. Sci. 1997; 22:87-92

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Authors
Russell, L. C., Whitt, S. R., Chen, M.-S., & Chinkers, M.
Affiliations
University of South Alabama (US)
Corresponding author
Chinkers, Michael
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
1999-07
Volume
274
Issue
29
Pages
20060-20063
ISSN
0021-9258 (ISSN-L)
OpenAlex
W1983211885
Cited by
165
References
36
Citations by year
2026: 3 · 2025: 2 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 7 · 2020: 2 · 2019: 2 · 2018: 3 · 2017: 3 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 7
FWCI
3.34
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01hl047063-08)
  • National Heart, Lung, and Blood Institute (HL47063)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK 55877, R01DK055877)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
HSP90, tetratricopeptide repeat domain, protein-protein binding, binding groove, acidic residues, molecular chaperones, site-directed mutagenesis
MeSH terms
Amino Acid Sequence, Animals, Cytoplasm, HeLa Cells, Humans, Peptides, Phosphoprotein Phosphatases, Protein Binding, Mutagenesis, Site-Directed, Conserved Sequence, HSP90 Heat-Shock Proteins, COS Cells

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