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Eosinophil Peroxidase Nitrates Protein Tyrosyl Residues
Open accessAbstract
Eosinophil peroxidase (EPO) has been implicated in promoting oxidative tissue injury in conditions ranging from asthma and other allergic inflammatory disorders to cancer and parasitic/helminthic infections. Studies thus far on this unique peroxidase have primarily focused on its unusual substrate preference for bromide (Br(-)) and the pseudohalide thiocyanate (SCN(-)) forming potent hypohalous acids as cytotoxic oxidants. However, the ability of EPO to generate reactive nitrogen species has not yet been reported. We now demonstrate that EPO readily uses nitrite (NO(2)(-)), a major end-product of nitric oxide ((.)NO) metabolism, as substrate to generate a reactive intermediate that nitrates protein tyrosyl residues in high yield. EPO-catalyzed nitration of tyrosine occurred more readily than bromination at neutral pH, plasma levels of halides, and pathophysiologically relevant concentrations of NO(2)(-). Furthermore, EPO was significantly more effective than MPO at promoting tyrosine nitration in the presence of plasma levels of halides. Whereas recent studies suggest that MPO can also promote protein nitration through indirect oxidation of NO(2)(-) with HOCl, we found no evidence that EPO can indirectly mediate protein nitration by a similar reaction between HOBr and NO(2)(-). EPO-dependent nitration of tyrosine was modulated over a physiologically relevant range of SCN(-) concentrations and was accompanied by formation of tyrosyl radical addition products (e.g. o,o'-dityrosine, pulcherosine, trityrosine). The potential role of specific antioxidants and nucleophilic scavengers on yields of tyrosine nitration and bromination by EPO are examined. Thus, EPO may contribute to nitrotyrosine formation in inflammatory conditions characterized by recruitment and activation of eosinophils.
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“Generation of nitrogen dioxide and subsequent protein nitration from nitrite oxidation by peroxidases such as myeloperoxidase[108], eosinophil peroxidase [109] and also other heme proteins such as cytochrome c [110], hemoglobin [111, 112] and myoglobin [112, 113] was appreciated many years before the biologic potential for nitrite-reduction to NO was realized.”
From Current perspectives and challenges in understanding the role of nitrite as an integral player in nitric oxide biology and therapy (2011) · cites this paper as [109]
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Metadata
- Authors
- Wu, W., Chen, Y., & Hazen, S. L.
- Affiliations
- Cleveland State University (US)
- Corresponding author
- Hazen, Stanley L.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 1999-09
- Volume
- 274
- Issue
- 36
- Pages
- 25933-25944
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2094921988
- Cited by
- 265
- References
- 70
- Citations by year
- 2026: 7 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 6 · 2016: 4 · 2015: 4 · 2014: 6 · 2013: 7 · 2012: 8
- FWCI
- 7.75
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Heart, Lung, and Blood Institute (HL61878, HL 62526, R01HL061878, R01HL062526)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry (medical) › Antioxidant Activity and Oxidative Stress
- Keywords
- eosinophil peroxidase, nitrite, tyrosine nitration, reactive nitrogen species, eosinophils, myeloperoxidase, thiocyanate, bromide, hypohalous acids, nitrotyrosine, oxidative tissue injury, antioxidants, eosinophil activation
- MeSH terms
- Animals, Eosinophils, Inflammation, Kinetics, Nitric Oxide, Nitrites, Peroxidases, Tyrosine, Oxidative Stress, Eosinophil Peroxidase
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