The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
The Bloom's Syndrome Gene Product Interacts with Topoisomerase III
Open accessAbstract
Bloom's syndrome is a rare genetic disorder associated with loss of genomic integrity and a large increase in the incidence of many types of cancer at an early age. The Bloom's syndrome gene product, BLM, belongs to the RecQ family of DNA helicases, which also includes the human Werner's and Rothmund-Thomson syndrome gene products and the Sgs1 protein of Saccharomyces cerevisiae. This family shows strong evolutionary conservation of protein structure and function. Previous studies have shown that Sgs1p interacts both physically and genetically with topoisomerase III. Here, we have investigated whether this interaction has been conserved in human cells. We show that BLM and hTOPO IIIalpha, one of two human topoisomerase III homologues, co-localize in the nucleus of human cells and can be co-immunoprecipitated from human cell extracts. Moreover, the purified BLM and hTOPO IIIalpha proteins are able to bind specifically to each other in vitro, indicating that the interaction is direct. We have mapped two independent domains on BLM that are important for mediating the interaction with hTOPO IIIalpha. Furthermore, through characterizing a genetic interaction between BLM and TOP3 in S. cerevisiae, we have identified a functional role for the hTOPO IIIalpha interaction domains in BLM.
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Cites
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Positional Cloning of the Werner's Syndrome Gene
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Mutations in RECQL4 cause a subset of cases of Rothmund-Thomson syndrome
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Cited by
Mackay, A., Burford, A., Carvalho, D., Izquierdo, E., Fazal-Salom, J., Taylor, K. R., Bjerke, L., Clarke, M., Vinci, M., Nandhabalan, M., et al. (2017) · Cancer Cell · doi:10.1016/j.ccell.2017.08.017 · Cited by 1,212
The Bloom's syndrome helicase suppresses crossing over during homologous recombination
Wu, L., & Hickson, I. D. (2003) · Nature · doi:10.1038/nature02253 · Cited by 1,085
Self-citationSgs1 Helicase and Two Nucleases Dna2 and Exo1 Resect DNA Double-Strand Break Ends
Zhu, Z., Chung, W.-H., Shim, E. Y., Lee, S. E., & Ira, G. (2008) · Cell · doi:10.1016/j.cell.2008.08.037 · Cited by 1,051
Tumor Cell Death Induced by Topoisomerase-Targeting Drugs
Li, T.-K., & Liu, L. F. (2001) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.41.1.53 · Cited by 508
DNA topoisomerases: harnessing and constraining energy to govern chromosome topology
Schoeffler, A. J., & Berger, J. M. (2008) · Quarterly Reviews of Biophysics · doi:10.1017/s003358350800468x · Cited by 490
Metadata
- Authors
- Wu, L., Davies, S. L., North, P. S., Goulaouic, H., Riou, J.-F., Turley, H., Gatter, K. C., & Hickson, I. D.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-03
- Volume
- 275
- Issue
- 13
- Pages
- 9636-9644
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2039674107
- Cited by
- 318
- References
- 36
- FWCI
- 10.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
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