The DOI matches its Crossref record; 66 of the 66 references it cites were found in a registry or an index.
Rho-A Is Critical for Osteoclast Podosome Organization, Motility, and Bone Resorption
Open accessAbstract
Rho plays a regulatory role in the formation of actin stress fibers and focal adhesions, and it is also involved in integrin-mediated signaling events. To study the role of Rho in alpha(v)beta(3)/gelsolin-dependent signaling, the HIV-Tat peptide, hemagglutinin (HA)-tagged Rho(Val-14) (constitutively active) and Rho(Asn-19) (dominant negative) were transduced into avian osteoclasts. Protein transduction by HA-Tat was highly efficient, and 90-100% of the cells were transduced with HA-tagged proteins. We demonstrate here that Rho(Val-14) transduction (100 nM) stimulated gelsolin-associated phosphatidylinositol 3-kinase activity, podosome assembly, stress fiber formation, osteoclast motility, and bone resorption, mimicking osteoclast stimulation by osteopontin/alpha(v)beta(3.) The effects of Rho(Val-14) transduction stimulation was time-dependent. C3 exoenzyme blocked the effects of Rho(Val-14) and induced podosome disassembly, loss of motility, and inhibition of bone resorption. Transduction of Rho(Asn-19) produced podosome disassembly, and blocked osteopontin stimulation. These data demonstrate that integrin-dependent activation of phosphoinositide synthesis, actin stress fiber formation, podosome reorganization for osteoclast motility, and bone resorption require Rho stimulation.
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Cited by
The multiple functions and mechanisms of osteopontin
Icer, M. A., & Gezmen-Karadag, M. (2018) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2018.07.003 · Cited by 606
The cell biology of bone metabolism
Datta, H. K., Ng, W. F., Walker, J. A., Tuck, S. P., & Varanasi, S. S. (2008) · Journal of Clinical Pathology · doi:10.1136/jcp.2007.048868 · Cited by 614
Rho signaling, ROCK and mDia1, in transformation, metastasis and invasion
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Glucocorticoids suppress bone formation via the osteoclast.
Kim, H.-J. (2006) · Journal of Clinical Investigation · doi:10.1172/jci28084 · Cited by 384
Apatite-mediated Actin Dynamics in Resorbing Osteoclasts
Saltel, F., Destaing, O., Bard, F., Eichert, D., & Jurdic, P. (2004) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-06-0522 · Cited by 267
Metadata
- Authors
- Chellaiah, M. A., Soga, N., Swanson, S., McAllister, S., Alvarez, U., Wang, D., Dowdy, S. F., & Hruska, K. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2000-04
- Volume
- 275
- Issue
- 16
- Pages
- 11993-12002
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2084819879
- Cited by
- 263
- References
- 66
- FWCI
- 5.75
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Chellaiah, M. A., Soga, N., Swanson, S., McAllister, S., Alvarez, U., Wang, D., Dowdy, S. F., & Hruska, K. A. (2000). Rho-A Is Critical for Osteoclast Podosome Organization, Motility, and Bone Resorption. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.275.16.11993
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