Cited by 267Bone Metabolism and DiseasesBone and Dental Protein StudiesCellular Mechanics and Interactions

Apatite-mediated Actin Dynamics in Resorbing Osteoclasts

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Saltel, Frédéric, Destaing, Olivier, Bard, Frédéric, Eichert, Diane, Jurdic, Pierre·2004·Molecular Biology of the Cell

Abstract

The actin cytoskeleton is essential for osteoclasts main function, bone resorption. Two different organizations of actin have been described in osteoclasts, the podosomes belt corresponding to numerous F-actin columns arranged at the cell periphery, and the sealing zone defined as a unique large band of actin. To compare the role of these two different actin organizations, we imaged osteoclasts on various substrata: glass, dentin, and apatite. Using primary osteoclasts expressing GFP-actin, we found that podosome belts and sealing zones, both very dynamic actin structures, were present in mature osteoclasts; podosome belts were observed only in spread osteoclasts adhering onto glass, whereas sealing zone were seen in apico-basal polarized osteoclasts adherent on mineralized matrix. Dynamic observations of several resorption cycles of osteoclasts seeded on apatite revealed that 1) podosomes do not fuse together to form the sealing zone; 2) osteoclasts alternate successive stationary polarized resorption phases with a sealing zone and migration, nonresorption phases without any specific actin structure; and 3) apatite itself promotes sealing zone formation though c-src and Rho signaling. Finally, our work suggests that apatite-mediated sealing zone formation is dependent on both c-src and Rho whereas apico-basal polarization requires only Rho.

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Crossref · 10.1091/mbc.e04-06-0522

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  • The interaction between osteoclasts and bone matrix is essential for osteoclast function, since bone resorption occurs only when osteoclasts bind to mineralized bone surface.

    “On the other hand, the interaction between osteoclasts and bone matrix is essential for osteoclast function, since as previously mentioned, bone resorption occurs only when osteoclasts bind to mineralized bone surface [97].”
  • These osteoclast membrane domains are only formed when osteoclasts are in contact with extracellular mineralized matrix, in a process in which αvβ3-integrin and CD44 mediate the attachment of osteoclast podosomes to the bone surface.

    “It is important to mention that these domains are only formed when osteoclasts are in contact with extracellular mineralized matrix, in a process which α v β 3-integrin, as well as the CD44, mediates the attachment of the osteoclast podosomes to the bone surface [96–99].”

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Research works printed but not linked: 2

  1. Lakkakorpi, P.T., and Vaananen, H.K. (1996). Cytoskeletal changes in osteoclasts during the resorption cycle. Microsc. Res. Tech. 33, 171–181. [DOI] [PubMed] [Google Scholar]
  2. Shibutani, T., Iwanaga, H., Imai, K., Kitago, M., Doi, Y., and Iwayama, Y. (2000). Use of glass slides coated with apatite-collagen complexes for measurement of osteoclastic resorption activity. J. Biomed. Mater. Res. 50, 153–159. [DOI] [PubMed] [Google Scholar]

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Authors
Saltel, F., Destaing, O., Bard, F., Eichert, D., & Jurdic, P.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • University of California System (US)
  • European Synchrotron Radiation Facility (FR)
Venue
Molecular Biology of the Cell
Published
2004-12
Volume
15
Issue
12
Pages
5231-5241
ISSN
  • 1059-1524 (ISSN-L)
  • 1939-4586
OpenAlex
W2005716633
Cited by
267
References
39
Citations by year
2026: 2 · 2025: 1 · 2024: 9 · 2023: 6 · 2022: 8 · 2021: 12 · 2020: 11 · 2019: 14 · 2018: 14 · 2017: 5 · 2016: 12 · 2015: 22 · 2014: 16 · 2013: 9 · 2012: 13
FWCI
5.21
Type
journal article
Language
en
Open access
Locations
  • Molecular Biology of the Cell (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ligue Contre le Cancer
  • Centre National de la Recherche Scientifique
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Health Sciences › Medicine › Rheumatology › Bone and Dental Protein Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Keywords
osteoclasts, bone resorption, actin cytoskeleton, sealing zone, podosomes, apatite, Src kinase, Rho GTPase signaling, cell migration, dentin, mineralized matrix
MeSH terms
CSK Tyrosine-Protein Kinase, Actins, Animals, Apatites, Bone Resorption, Cell Differentiation, Cell Movement, Cells, Cultured, Humans, Male, Microscopy, Electron, Scanning, Osteoclasts, Protein-Tyrosine Kinases, Cell Polarity, src-Family Kinases, rho GTP-Binding Proteins, Mice

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