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Cited by 337

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Caveolae-deficient Endothelial Cells Show Defects in the Uptake and Transport of Albumin in Vivo

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Schubert, William, Frank, Philippe G., Razani, Babak, Park, David S., Chow, Chi-Wing, Lisanti, Michael P.·2001·Journal of Biological Chemistry
DOI 10.1074/jbc.c100613200
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Abstract

The role of endothelial cell caveolae in the uptake and transport of macromolecules from the blood-space to the tissue-space remains controversial. To address this issue directly, we employed caveolin-1 gene knock-out mice that lack caveolin-1 protein expression and caveolae organelles. Here, we show that endothelial cell caveolae are required for the efficient uptake and transport of a known caveolar ligand, i.e. albumin, in vivo. Caveolin-1-null mice were perfused with 5-nm gold-conjugated albumin, and its uptake was followed by transmission electron microscopy. Our results indicate that gold-conjugated albumin is not endocytosed by Cav-1-deficient lung endothelial cells and remains in the blood vessel lumen; in contrast, gold-conjugated albumin was concentrated and internalized by lung endothelial cell caveolae in wild-type mice, as expected. To quantitate this defect in uptake, we next studied the endocytosis of radioiodinated albumin using aortic ring segments from wild-type and Cav-1-null mice. Interestingly, little or no uptake of radioiodinated albumin was observed in the aortic segments from Cav-1-deficient mice, whereas aortic segments from wild-type mice showed robust uptake that was time- and temperature-dependent and competed by unlabeled albumin. We conclude that endothelial cell caveolae are required for the efficient uptake and transport of albumin from the blood to the interstitium.

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Metadata

Authors
Schubert, W., Frank, P. G., Razani, B., Park, D. S., Chow, C.-W., & Lisanti, M. P.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-12
Volume
276
Issue
52
Pages
48619-48622
ISSN
0021-9258 (ISSN-L)
DOI
10.1074/jbc.c100613200
OpenAlex
W2019862207
Cited by
337
References
47
FWCI
5.06
Type
journal article
Language
en
Open access
hybrid

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