Cernunnos Interacts with the XRCC4·DNA-ligase IV Complex and Is Homologous to the Yeast Nonhomologous End-joining Factor Nej1
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DNA double strand breaks are considered as the most harmful DNA lesions and are repaired by either homologous recombination or nonhomologous end joining (NHEJ). A new NHEJ factor, Cernunnos, has been identified, the defect of which leads to a severe immunodeficiency condition associated with microcephaly and other developmental defects in humans. This presentation is reminiscent to that of DNA-ligase IV deficiency and suggests a possible interplay between Cernunnos and the XRCC4 x DNA-ligase IV complex. We show here that Cernunnos physically interacts with the XRCC4 x DNA-ligase IV complex. Moreover, a combination of sensitive methods of sequence analysis revealed that Cernunnos can be associated with the XRCC4 family of proteins and that it corresponds to the genuine homolog of the yeast Nej1 protein. Altogether these results shed new lights on the last step, the DNA religation, of the NHEJ pathway.
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DNA ligase IV completes the final steps of NHEJ to ligate DNA ends together while being held with XLF/XRCC4 and Ku/XRCC4 complexes.
“DNA ligase IV completes the final steps of NHEJ to ligate DNA ends together while being held with XLF/XRCC4 and Ku/XRCC4 complexes [68,69].”
From Integration of DNA damage and repair with murine double-minute 2 (Mdm2) in tumorigenesis (2012) · cites this paper as [69]
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- Authors
- Callebaut, I., Malivert, L., Fischer, A., Mornon, J.-P., Revy, P., & de Villartay, J.-P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Hôpital Necker-Enfants Malades (FR)
- Corresponding author
- Callebaut, Isabelle
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2006-05
- Volume
- 281
- Issue
- 20
- Pages
- 13857-13860
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2024144876
- Cited by
- 130
- References
- 30
- Citations by year
- 2026: 1 · 2025: 2 · 2023: 3 · 2022: 4 · 2021: 4 · 2020: 2 · 2019: 2 · 2017: 2 · 2016: 8 · 2015: 8 · 2014: 8 · 2013: 5 · 2012: 9
- FWCI
- 8.11
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- Cernunnos, XRCC4, DNA ligase IV, non-homologous end joining, DNA double-strand break repair, DNA religation, protein-protein interaction, homology, immunodeficiency, microcephaly, DNA repair
- MeSH terms
- DNA Ligase ATP, Amino Acid Sequence, Cell Line, DNA Repair, DNA-Binding Proteins, Humans, Models, Molecular, Molecular Sequence Data, DNA Ligases, Protein Binding, Protein Conformation, Sequence Homology, Amino Acid, Saccharomyces cerevisiae Proteins, DNA Repair Enzymes
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