Cited by 116DNA Repair MechanismsEnzyme Structure and FunctionProtein Structure and Dynamics

XLF regulates filament architecture of the XRCC4.ligase IV complex

Open access
Hammel, Michal, Yu, Yaping, Fang, Shujuan, Lees-Miller, Susan P., Tainer, John A.·2010·Structure

Abstract

DNA ligase IV (LigIV) is critical for nonhomologous end joining (NHEJ), the major DNA double-strand break (DSB) repair pathway in human cells, and LigIV activity is regulated by XRCC4 and XLF (XRCC4-like factor) interactions. Here, we employ small angle X-ray scattering (SAXS) data to characterize three-dimensional arrangements in solution for full-length XRCC4, XRCC4 in complex with LigIV tandem BRCT domains and XLF, plus the XRCC4·XLF·BRCT2 complex. XRCC4 forms tetramers mediated through a head-to-head interface, and the XRCC4 C-terminal coiled-coil region folds back on itself to support this interaction. The interaction between XLF and XRCC4 is also mediated via head-to-head interactions. In the XLF·XRCC4·BRCT complex, alternating repeating units of XLF and XRCC4·BRCT place the BRCT domain on one side of the filament. Collective results identify XRCC4 and XLF filaments suitable to align DNA molecules and function to facilitate LigIV end joining required for DSB repair in vivo.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.str.2010.09.009

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Hammel, M., Yu, Y., Fang, S., Lees-Miller, S. P., & Tainer, J. A.
Affiliations
  • Lawrence Berkeley National Laboratory (US)
  • University of Calgary (CA)
  • Scripps Research Institute (US)
Corresponding author
  • Hammel, Michal
  • Tainer, John A.
Venue
Structure
Publisher
Elsevier BV
Published
2010-11
Volume
18
Issue
11
Pages
1431-1442
ISSN
0969-2126 (ISSN-L)
OpenAlex
W2013663837
Cited by
116
References
49
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 6 · 2022: 6 · 2021: 9 · 2020: 6 · 2019: 9 · 2018: 10 · 2017: 7 · 2016: 6 · 2015: 9 · 2014: 12 · 2013: 10 · 2012: 13
FWCI
4.17
Type
journal article
Language
en
Open access
Locations
  • Structure (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institutes of Health (69139, CA92584)
  • Canadian Institutes of Health Research
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
Keywords
X-ray luminosity function, XRCC4, DNA ligase IV, non-homologous end joining, DNA double-strand break repair, BRCT domain, protein-protein interaction, coiled coil, tetramerization, DNA end joining, DNA sequence alignment
MeSH terms
DNA Ligase ATP, Chromatography, Gel, DNA-Binding Proteins, Humans, Models, Molecular, DNA Ligases, Protein Conformation, DNA Repair Enzymes, Scattering, Small Angle, Molecular Dynamics Simulation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review