The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
The Mechanism of PAK Activation
Open accessAbstract
The p21-activated kinases (PAKs), in common with many kinases, undergo multiple autophosphorylation events upon interaction with appropriate activators. The Cdc42-induced phosphorylation of PAK serves in part to dissociate the kinase from its partners PIX and Nck. Here we investigate in detail how autophosphorylation events affect the catalytic activity of PAK by altering the autophosphorylation sites in both alpha- and betaPAK. Both in vivo and in vitro analyses demonstrate that, although most phosphorylation events in the PAK N-terminal regulatory domain play no direct role in activation, a phosphorylation of alphaPAK serine 144 or betaPAK serine 139, which lie in the kinase inhibitory domain, significantly contribute to activation. By contrast, sphingosine-mediated activation is independent of this residue, indicating a different mode of activation. Thus two autophosphorylation sites direct activation while three others control association with focal complexes via PIX and Nck.
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A brain serine/threonine protein kinase activated by Cdc42 and Rac1
Manser, E., Leung, T., Salihuddin, H., Zhao, Z.-S., & Lim, L. (1994) · Nature · doi:10.1038/367040a0 · Cited by 1,538
Self-citationPAK Kinases Are Directly Coupled to the PIX Family of Nucleotide Exchange Factors
Manser, E., Loo, T.-H., Koh, C.-G., Zhao, Z.-S., Chen, X.-Q., Tan, L., Tan, I., Leung, T., & Lim, L. (1998) · Molecular Cell · doi:10.1016/s1097-2765(00)80019-2 · Cited by 753
Self-citationRudel, T., & Bokoch, G. M. (1997) · Science · doi:10.1126/science.276.5318.1571 · Cited by 747
Integrin-mediated Signals Regulated by Members of the Rho Family of GTPases
Clark, E. A., King, W. G., Brugge, J. S., Symons, M., & Hynes, R. O. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.2.573 · Cited by 603
Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase
Sanders, L. C., Matsumura, F., Bokoch, G. M., & de Lanerolle, P. (1999) · Science · doi:10.1126/science.283.5410.2083 · Cited by 593
Expression of Constitutively Active α-PAK Reveals Effects of the Kinase on Actin and Focal Complexes
Manser, E., Huang, H.-Y., Loo, T.-H., Chen, X.-Q., Dong, J.-M., Leung, T., & Lim, L. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.3.1129 · Cited by 561
Self-citationStructure of PAK1 in an Autoinhibited Conformation Reveals a Multistage Activation Switch
Lei, M., Lu, W., Meng, W., Parrini, M.-C., Eck, M. J., Mayer, B. J., & Harrison, S. C. (2000) · Cell · doi:10.1016/s0092-8674(00)00043-x · Cited by 545
del Pozo, M. A. (2000) · The EMBO Journal · doi:10.1093/emboj/19.9.2008 · Cited by 452
The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338
King, A. J., Sun, H., Diaz, B., Barnard, D., Miao, W., Bagrodia, S., & Marshall, M. S. (1998) · Nature · doi:10.1038/24184 · Cited by 443
Bagrodia, S., & Cerione, R. A. (1999) · Trends in Cell Biology · doi:10.1016/s0962-8924(99)01618-9 · Cited by 393
Cited by
Deacon, S. W., Beeser, A., Fukui, J. A., Rennefahrt, U. E. E., Myers, C., Chernoff, J., & Peterson, J. R. (2008) · Chemistry & Biology · doi:10.1016/j.chembiol.2008.03.005 · Cited by 366
Arias‐Romero, L. E., & Chernoff, J. (2008) · Biology of the Cell · doi:10.1042/bc20070109 · Cited by 364
Role of p21-activated kinase pathway defects in the cognitive deficits of Alzheimer disease
Zhao, L., Ma, Q.-L., Calon, F., Harris-White, M. E., Yang, F., Lim, G. P., Morihara, T., Ubeda, O. J., Ambegaokar, S., Hansen, J. E., et al. (2006) · Nature Neuroscience · doi:10.1038/nn1630 · Cited by 317
Changes in Synaptic Morphology Accompany Actin Signaling during LTP
Chen, L. Y., Rex, C. S., Casale, M. S., Gall, C. M., & Lynch, G. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.0164-07.2007 · Cited by 296
Rex, C. S., Chen, L. Y., Sharma, A., Liu, J., Babayan, A. H., Gall, C. M., & Lynch, G. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200901084 · Cited by 293
Metadata
- Authors
- Chong, C., Tan, L., Lim, L., & Manser, E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-05
- Volume
- 276
- Issue
- 20
- Pages
- 17347-17353
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1964579181
- Cited by
- 258
- References
- 36
- FWCI
- 5.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
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