The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
Mechanisms of pH Regulation in the Regulated Secretory Pathway
Open accessAbstract
A precise pH gradient between organelles of the regulated secretory pathway is required for sorting and processing of prohormones. We studied pH regulation in live endocrine cells by targeting biotin-based pH indicators to cellular organelles expressing avidin-chimera proteins. In AtT-20 cells, we found that steady-state pH decreased from the endoplasmic reticulum (ER) (pH(ER) = 7.4 +/- 0.2, mean +/- S.D.) to Golgi (pH(G) = 6.2 +/- 0.4) to mature secretory granules (MSGs) (pH(MSG) = 5.5 +/- 0.4). Golgi and MSGs required active H(+) v-ATPases for acidification. ER, Golgi, and MSG steady-state pH values were also dependent upon the different H(+) leak rates across each membrane. However, neither steady-state pH(MSG) nor rates of passive H(+) leak were affected by Cl(-)-free solutions or valinomycin, indicating that MSG membrane potential was small and not a determinant of pH(MSG). Therefore, our data do not support earlier suggestions that organelle acidification is primarily regulated by Cl(-) conductances. Measurements of H(+) leak rates, buffer capacities, and estimates of surface areas and volumes of these organelles were applied to a mathematical model to determine the H(+) permeability (P(H+)) of each organelle membrane. We found that P(H+) decreased progressively from ER to Golgi to MSGs, and proper acidification of Golgi and MSGs required gradual decreases in P(H+) and successive increases in the active H(+) pump density.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
An Introduction to Error Analysis
Taylor, J. R. (1982) · Medical Entomology and Zoology · Cited by 2,807
Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
Miesenböck, G., De Angelis, D. A., & Rothman, J. E. (1998) · Nature · doi:10.1038/28190 · Cited by 2,563
Roos, A., & Boron, W. F. (1981) · Physiological Reviews · doi:10.1152/physrev.1981.61.2.296 · Cited by 2,511
Llopis, J., McCaffery, J. M., Miyawaki, A., Farquhar, M. G., & Tsien, R. Y. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.6803 · Cited by 1,130
Self-citationPaula, S., Volkov, A. G., Van Hoek, A. N., Haines, T. H., & Deamer, D. W. (1996) · Biophysical Journal · doi:10.1016/s0006-3495(96)79575-9 · Cited by 578
Sorting and storage during secretory granule biogenesis: looking backward and looking forward
ARVAN, P., & CASTLE, D. (1998) · Biochemical Journal · doi:10.1042/bj3320593 · Cited by 523
Stobrawa, S. M., Breiderhoff, T., Takamori, S., Engel, D., Schweizer, M., Zdebik, A. A., Bösl, M. R., Ruether, K., Jahn, H., Draguhn, A., et al. (2001) · Neuron · doi:10.1016/s0896-6273(01)00189-1 · Cited by 508
Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network.
Chanat, E., & Huttner, W. B. (1991) · The Journal of cell biology · doi:10.1083/jcb.115.6.1505 · Cited by 458
Maycox, P. R., Deckwerth, T., Hell, J. W., & Jahn, R. (1988) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)37605-7 · Cited by 361
Vesicles and cisternae in the trans golgi apparatus of human fibroblasts are acidic compartments
Anderson, R. G. W., & Pathak, R. K. (1985) · Cell · doi:10.1016/0092-8674(85)90212-0 · Cited by 349
Cited by
Sensors and regulators of intracellular pH
Casey, J. R., Grinstein, S., & Orlowski, J. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2820 · Cited by 2,218
Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts
Stewart, M. P., Langer, R., & Jensen, K. F. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00678 · Cited by 747
The role of pyocyanin in Pseudomonas aeruginosa infection
Lau, G. W., Hassett, D. J., Ran, H., & Kong, F. (2004) · Trends in Molecular Medicine · doi:10.1016/j.molmed.2004.10.002 · Cited by 688
Nakamura, N., Tanaka, S., Teko, Y., Mitsui, K., & Kanazawa, H. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m410041200 · Cited by 353
Dense-core secretory granule biogenesis
Kim, T., Gondré-Lewis, M. C., Arnaoutova, I., & Loh, Y. P. (2006) · Physiology · doi:10.1152/physiol.00043.2005 · Cited by 203
Metadata
- Authors
- Wu, M. M., Grabe, M., Adams, S., Tsien, R. Y., Moore, H.-P. H., & Machen, T. E.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2001-08
- Volume
- 276
- Issue
- 35
- Pages
- 33027-33035
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2162613182
- Cited by
- 266
- References
- 36
- FWCI
- 4.95
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Mechanisms of pH Regulation in the Regulated Secretory Pathway. https://app.cochat.ai/papers/10.1074/jbc.m103917200
- The paper
- Wu, M. M., Grabe, M., Adams, S., Tsien, R. Y., Moore, H.-P. H., & Machen, T. E. (2001). Mechanisms of pH Regulation in the Regulated Secretory Pathway. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m103917200
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
