Human Mitochondrial Thioredoxin
Open accessAbstract
Thioredoxins (Trx) are a class of small multifunctional redox-active proteins found in all organisms. Recently, we reported the cloning of a mitochondrial thioredoxin, Trx2, from rat heart. To investigate the biological role of Trx2 we have isolated the human homologue, hTrx2, and generated HEK-293 cells overexpressing Trx2 (HEK-Trx2). Here, we show that HEK-Trx2 cells are more resistant toward etoposide. In addition, HEK-Trx2 are more sensitive toward rotenone, an inhibitor of complex I of the respiratory chain. Finally, overexpression of Trx2 confers an increase in mitochondrial membrane potential, DeltaPsi(m). Treatment with oligomycin could both reverse the effect of rotenone and decrease the membrane potential suggesting that Trx2 interferes with the activity of ATP synthase. Taken together, these results suggest that Trx2 interacts with specific components of the mitochondrial respiratory chain and plays an important role in the regulation of the mitochondrial membrane potential.
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JC-1 accumulates in mitochondria in a potential-dependent manner.
“JC-1 is a cationic fluorescent dye (green color as monomer; 539 nm) that accumulates in mitochondria in a potential-dependent manner.”
From Mitofusin-2 Determines Mitochondrial Network Architecture and Mitochondrial Metabolism (2003) · cites this paper as [29]
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Thioredoxin‐2 (TRX‐2) is an essential gene regulating mitochondria‐dependent apoptosis
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- Authors
- Damdimopoulos, A. E., Miranda-Vizuete, A., Pelto-Huikko, M., Gustafsson, J.-Å., & Spyrou, G.
- Affiliations
- Karolinska Institutet (SE)
- Corresponding author
- Spyrou, Giannis
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-09
- Volume
- 277
- Issue
- 36
- Pages
- 33249-33257
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2072623280
- Cited by
- 192
- References
- 1
- Citations by year
- 2025: 1 · 2024: 4 · 2023: 4 · 2022: 5 · 2021: 3 · 2020: 8 · 2019: 6 · 2018: 2 · 2017: 5 · 2016: 7 · 2015: 2 · 2014: 11 · 2013: 5 · 2012: 13
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- mitochondrial thioredoxin, Trx2, human homologue, redox proteins, etoposide resistance, rotenone, mitochondrial complex I inhibition, mitochondrial membrane potential, oligomycin, ATP synthase, electron transport chain
- MeSH terms
- Amino Acid Sequence, Cell Line, Cloning, Molecular, Dose-Response Relationship, Drug, Enzyme Inhibitors, Etoposide, Humans, Immunohistochemistry, Membrane Potentials, Microscopy, Fluorescence, Mitochondria, Molecular Sequence Data, Oligomycins, Protein Binding, RNA, Messenger, Rotenone, Thioredoxins, Time Factors, Tissue Distribution, Transfection, Blotting, Western, Cell Death, Sequence Homology, Amino Acid, DNA, Complementary, Mitochondrial Proteins, Mitochondrial Proton-Translocating ATPases
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