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Large Scale Gene Expression Analysis of Osteoclastogenesisin Vitro and Elucidation of NFAT2 as a Key Regulator
Open accessAbstract
To understand the molecular events coupling between cell proliferation and differentiation by elucidating genes essential for the process, we conducted a large scale gene expression analysis of an in vitro osteoclastogenesis system consisting of recombinant RANKL and mouse RAW264 cells. The entire process leading to the formation of tartrate resistant acid phosphatase-positive multinucleated cells takes 3 days and plates become fully covered with multinucleated cells at 4 days. Microarray probing at eight time points revealed 635 genes that showed greater than 2-fold differential expression for at least one time point and they could be classified into six groups by the "k-means" clustering analysis. Among a group of 106 early inducible genes (within 2-5 h after RANKL stimulation), four genes including NFAT2 were identified as genes whose enhanced expressions were fairly correlated with an efficient induction of matured osteoclasts. Moreover, cyclosporin A significantly suppressed the multinucleated cell formation accompanying the reduction of the nuclear localization of NFAT2. When the expression of NFAT2 was suppressed by introducing antisense NFAT2, multinucleated cell formation was severely hampered. Functional analysis thus combined with gene analysis by microarray technology elucidated a key role of NFAT2 in osteoclastogenesis in vitro.
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“NFATc1 is a transcription factor that plays a master role in osteoclastogenesis168 by regulating the expression of osteoclast-specific genes such as cathepsin K, DC-STAMP, and tartrate-resistant acid phosphatase (TRAP) or that of osteoclast-associated receptor (OSCAR) and other genes involved in OC resorptive functions.169”
From Bone remodeling: an operational process ensuring survival and bone mechanical competence (2022) · cites this paper as [168]
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Cites
Kohonen, T. (1995) · Springer series in information sciences · doi:10.1007/978-3-642-56927-2 · Cited by 16,032
Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
Schena, M., Shalon, D., Davis, R. W., & Brown, P. O. (1995) · Science · doi:10.1126/science.270.5235.467 · Cited by 9,609
(1975) · CERN Document Server (European Organization for Nuclear Research) · Cited by 5,958
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation.
Lacey, D. L., Timms, E., Tan, H.-L., Kelley, M. J., Dunstan, C. R., Burgess, T., Elliott, R., Colombero, A., Elliott, G., Scully, S., et al. (1998) · Cell · doi:10.1016/s0092-8674(00)81569-x · Cited by 5,439
Yasuda, H., Shima, N., Nakagawa, N., Yamaguchi, K., Kinosaki, M., Mochizuki, S.-I., Tomoyasu, A., Yano, K., Goto, M., Murakami, A., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.7.3597 · Cited by 4,224
Calcineurin is a common target of cyclophilin-cyclosporin A and FKBP-FK506 complexes
Liu, J., Farmer, J. D., Lane, W. S., Friedman, J., Weissman, I., & Schreiber, S. L. (1991) · Cell · doi:10.1016/0092-8674(91)90124-h · Cited by 4,116
Serial Analysis of Gene Expression
Velculescu, V. E., Zhang, L., Vogelstein, B., & Kinzler, K. W. (1995) · Science · doi:10.1126/science.270.5235.484 · Cited by 3,713
Alessi, D. R., Cuenda, A., Cohen, P., Dudley, D. T., & Saltiel, A. R. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.46.27489 · Cited by 3,374
Expression monitoring by hybridization to high-density oligonucleotide arrays
Lockhart, D. J., Dong, H., Byrne, M. C., Follettie, M. T., Gallo, M. V., Chee, M. S., Mittmann, M., Wang, C., Kobayashi, M., Norton, H., et al. (1996) · Nature Biotechnology · doi:10.1038/nbt1296-1675 · Cited by 2,841
TRANSCRIPTION FACTORS OF THE NFAT FAMILY:Regulation and Function
Rao, A., Luo, C. H., & Hogan, P. G. (1997) · Annual Review of Immunology · doi:10.1146/annurev.immunol.15.1.707 · Cited by 2,592
Transcriptional Activation by Tetracyclines in Mammalian Cells
Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W., & Bujard, H. (1995) · Science · doi:10.1126/science.7792603 · Cited by 2,488
Suda, T., Takahashi, N., Udagawa, N., Jimi, E., Gillespie, M. T., & Martin, T. J. (1999) · Endocrine Reviews · doi:10.1210/edrv.20.3.0367 · Cited by 2,345
A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function.
Anderson, D. M., Maraskovsky, E., Billingsley, W. L., Dougall, W. C., Tometsko, M. E., Roux, E. R., Teepe, M. C., DuBose, R. F., Cosman, D., & Galibert, L. (1997) · Nature · doi:10.1038/36593 · Cited by 2,290
Hsu, H., Lacey, D. L., Dunstan, C. R., Solovyev, I., Colombero, A., Timms, E., Tan, H.-L., Elliott, G., Kelley, M. J., Sarosi, I., et al. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.3540 · Cited by 1,636
Identification of calcineurin as a key signalling enzyme in T-lymphocyte activation
Clipstone, N. A., & Crabtree, G. R. (1992) · Nature · doi:10.1038/357695a0 · Cited by 1,635
Computational analysis of microarray data
Quackenbush, J. (2001) · Nature Reviews Genetics · doi:10.1038/35076576 · Cited by 1,481
Sibson, R., & Hartigan, J. A. (1976) · Journal of the Royal Statistical Society Series C (Applied Statistics) · doi:10.2307/2346526 · Cited by 1,457
T-Cell mediated regulation of osteoclastogenesis by signalling cross-talk between RANKL and IFN-γ.
Takayanagi, H., Ogasawara, K., Hida, S., Chiba, T., Murata, S., Sato, K., Takaoka, A., Yokochi, T., Oda, H., Tanaka, K., et al. (2000) · Nature · doi:10.1038/35046102 · Cited by 1,358
TRAF6 deficiency results in osteopetrosis and defective interleukin-1, CD40, and LPS signalingPreprint
Lomaga, M. A., Yeh, W., Sarosi, I., Duncan, G. S., Furlonger, C. L., Ho, A. P., Morony, S., Capparelli, C., Van, G. Y., Kaufman, S., et al. (1999) · Genes & Development · doi:10.1101/gad.13.8.1015 · Cited by 1,265
c-Fos: a Key Regulator of Osteoclast-Macrophage Lineage Determination and Bone Remodeling
Grigoriadis, A. E., Wang, Z., Cecchini, M. G., Hofstetter, W., Felix, R., Fleisch, H. A., & Wagner, E. F. (1994) · Science · doi:10.1126/science.7939685 · Cited by 1,264
Ninomiya‐Tsuji, J., Kishimoto, K., Hiyama, A., Inoue, J., Cao, Z., & Matsumoto, K. (1999) · Nature · doi:10.1038/18465 · Cited by 1,187
TRANSCRIPTIONAL REGULATION OF GENE EXPRESSION DURING ADIPOCYTE DIFFERENTIATION
MacDougald, O. A., & Lane, M. D. (1995) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.64.070195.002021 · Cited by 1,064
Wong, B. R., Rho, J., Arron, J. R., Robinson, E. A., Orlinick, J. R., Chao, M. V., Kalachikov, S. M., Cayani, E., Bartlett, F. S., Frankel, W. N., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.40.25190 · Cited by 1,042
MAPKs: new JNK expands the group
Davis, R. J. (1994) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(94)90132-5 · Cited by 944
JNK is involved in signal integration during costimulation of T lymphocytes
Su, B., Jacinto, E., Hibi, M., Kallunki, T., Karin, M., & Ben‐Neriah, Y. (1994) · Cell · doi:10.1016/0092-8674(94)90056-6 · Cited by 907
Partners in transcription: NFAT and AP-1
Macián, F., López‐Rodríguez, C., & Rao, A. (2001) · Oncogene · doi:10.1038/sj.onc.1204386 · Cited by 766
Naito, A., Azuma, S., Tanaka, S., Miyazaki, T., Takaki, S., Takatsu, K., Nakao, K., Nakamura, K., Katsuki, M., Yamamoto, T. S., et al. (1999) · Genes to Cells · doi:10.1046/j.1365-2443.1999.00265.x · Cited by 626
Assuma, R., Oates, T. W., Cochran, D. L., Amar, S., & Graves, D. T. (1998) · The Journal of Immunology · doi:10.4049/jimmunol.160.1.403 · Cited by 598
NF-AT components define a family of transcription factors targeted in T-cell activation
Northrop, J. P., Ho, S. N., Chen, L., Thomas, D. J., Timmerman, L., Nolan, G. P., Admon, A., & Crabtree, G. R. (1994) · Nature · doi:10.1038/369497a0 · Cited by 573
Advances in Bone Biology: The Osteoclast*
Roodman, G. D. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-4-308 · Cited by 553
Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression
Timmerman, L., Clipstone, N. A., Ho, S. N., Northrop, J. P., & Crabtree, G. R. (1996) · Nature · doi:10.1038/383837a0 · Cited by 528
Matsumoto, M., SUDO, T., Saito, T., Osada, H., & Tsujimoto, M. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m001229200 · Cited by 513
Shibasaki, F., Price, E. R., Milan, D. J., & McKeon, F. (1996) · Nature · doi:10.1038/382370a0 · Cited by 487
Segregation of TRAF6‐mediated signaling pathways clarifies its role in osteoclastogenesis
Kobayashi, N., Kadono, Y., Naito, A., Matsumoto, K., Yamamoto, T. S., Tanaka, S., & Inoue, J. (2001) · The EMBO Journal · doi:10.1093/emboj/20.6.1271 · Cited by 473
DSCR1, overexpressed in Down syndrome, is an inhibitor of calcineurin-mediated signaling pathways
Fuentes, J. –. (2000) · Human Molecular Genetics · doi:10.1093/hmg/9.11.1681 · Cited by 465
SOCS: physiological suppressors of cytokine signaling
Krebs, D. L., & Hilton, D. J. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.16.2813 · Cited by 463
Takahashi, N., Udagawa, N., & Suda, T. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1999.0252 · Cited by 461
The TRAF Family of Signal Transducers Mediates NF-κB Activation by the TRANCE Receptor
Wong, B. R., Josien, R., Lee, S. Y., Vologodskaia, M., Steinman, R. M., & Choi, Y. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.43.28355 · Cited by 458
Beals, C. R., Clipstone, N. A., Ho, S. N., & Crabtree, G. R. (1997) · Genes & Development · doi:10.1101/gad.11.7.824 · Cited by 402
Molecular mechanisms regulating myogenic determination and differentiation
Robert, L. S. P. (2000) · Frontiers in bioscience · doi:10.2741/perry · Cited by 369
Fosl1 is a transcriptional target of c-Fos during osteoclast differentiation
Matsuo, K., Owens, J., Tonko, M., Elliott, C., Chambers, T. J., & Wagner, E. F. (2000) · Nature Genetics · doi:10.1038/72855 · Cited by 342
ECSIT is an evolutionarily conserved intermediate in the Toll/IL-1 signal transduction pathway
Kopp, E., Medzhitov, R., Carothers, J., Xiao, C., Douglas, I., Janeway, C. A., & Ghosh, S. (1999) · Genes & Development · doi:10.1101/gad.13.16.2059 · Cited by 334
A conserved family of calcineurin regulatorsPreprint
Kingsbury, T. J., & Cunningham, K. W. (2000) · Genes & Development · doi:10.1101/gad.14.13.1595 · Cited by 305
Calcineurin Binds the Transcription Factor NFAT1 and Reversibly Regulates Its Activity
Loh, C., Shaw, K. T. Y., Carew, J. A., Viola, J. P. B., Luo, C., Perrino, B. A., & Rao, A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.18.10884 · Cited by 299
Ralph, P., & Nakoinz, I. (1977) · The Journal of Immunology · doi:10.4049/jimmunol.119.3.950 · Cited by 279
Calcineurin preferentially synergizes with PKC‐θ to activate JNK and IL‐2 promoter in T lymphocytes
Werlen, G., Jacinto, E., Xia, Y., & Karin, M. (1998) · The EMBO Journal · doi:10.1093/emboj/17.11.3101 · Cited by 261
Serfling, E., Berberich‐Siebelt, F., Chuvpilo, S. A., Jankevics, E., Klein‐Hessling, S., Twardzik, T., & Avots, A. (2000) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/s0167-4889(00)00082-3 · Cited by 213
Matsuda, S., Moriguchi, T., Koyasu, S., & Nishida, E. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.20.12378 · Cited by 213
Cappellen, D., Luong-Nguyen, N.-H., Bongiovanni, S., Grenet, O., Wanke, C., & Šuša, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m200434200 · Cited by 184
Regulation of Nuclear Localization during Signaling
Cyert, M. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.r100012200 · Cited by 170
Porter, C. M., Havens, M. A., & Clipstone, N. A. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.5.3543 · Cited by 151
Regulation of the Murine Nfatc1 Gene by NFATc2
Zhou, B., Cron, R. Q., Wu, B., Genin, A., Wang, Z., Liu, S., Robson, P., & Baldwin, H. S. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m107068200 · Cited by 124
Cooperation between Syk and Rac1 Leads to Synergistic JNK Activation in T Lymphocytes
Jacinto, E., Werlen, G., & Karin, M. (1998) · Immunity · doi:10.1016/s1074-7613(00)80456-2 · Cited by 121
IL‐10 attenuates IFN‐α‐activated STAT1 in the liver: involvement of SOCS2 and SOCS3
Shen, X., Hong, F., Nguyen, V.-A., & Gao, B. (2000) · FEBS Letters · doi:10.1016/s0014-5793(00)01905-0 · Cited by 103
Characterization of the mouse tartrate-resistant acid phosphatase (trap) gene promoter
Reddy, S. V., Hundley, J. E., Windle, J. J., Alcantara, O., Linn, R. A., Leach, R. J., Boldt, D. H., & Roodman, G. D. (1995) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650100413 · Cited by 85
Differentiation-Related Changes in the Cell Cycle Traverse
Studzinski, G. P., & Harrison, L. E. (1999) · International review of cytology · doi:10.1016/s0074-7696(08)61384-4 · Cited by 67
Itahana, K., Morikazu, Y., & Takeya, T. (1996) · Endocrinology · doi:10.1210/endo.137.11.8895378 · Cited by 63
Self-citationAwumey, E. M., Moonga, B. S., Sodam, B. R., Koval, A. P., Adebanjo, O. A., Kumegawa, M., Zaidi, M., & Epstein, S. (1999) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1998.9785 · Cited by 55
Self-citationMeiyanto, E., Hoshijima, M., Ogawa, T., Ishida, N., & Takeya, T. (2001) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.2001.4564 · Cited by 39
Self-citationEffects of cyclosporine a on chick osteoclastsIn vitro
Chowdhury, M. H., Shen, V., & Dempster, D. W. (1991) · Calcified Tissue International · doi:10.1007/bf02556217 · Cited by 38
Hommes, F. A. (1962) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(62)90272-3 · Cited by 29
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Boyle, W. J., Simonet, W. S., & Lacey, D. L. (2003) · Nature · doi:10.1038/nature01658 · Cited by 6,670
Takayanagi, H., Kim, S., Koga, T., Nishina, H., Isshiki, M., Yoshida, H., Saiura, A., Isobe, M., Yokochi, T., Inoue, J.-I., et al. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00369-6 · Cited by 2,701
Transcriptional regulation by calcium, calcineurin, and NFAT
Hogan, P. G., Chen, L., Nardone, J., & Rao, A. (2003) · Genes & Development · doi:10.1101/gad.1102703 · Cited by 1,991
Genetic regulation of osteoclast development and function
Teitelbaum, S. L., & Ross, F. P. (2003) · Nature Reviews Genetics · doi:10.1038/nrg1122 · Cited by 1,720
The molecular understanding of osteoclast differentiation
Asagiri, M., & Takayanagi, H. (2007) · Bone · doi:10.1016/j.bone.2006.09.023 · Cited by 1,363
Metadata
- Authors
- Ishida, N., Hayashi, K., Hoshijima, M., Ogawa, T., Koga, S., Miyatake, Y., Kumegawa, M., Kimura, T., & Takeya, T.
- Affiliations
- Nara Institute of Science and Technology (JP)
- Meikai University (JP)
- Corresponding author
- Takeya, Tatsuo
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-10
- Volume
- 277
- Issue
- 43
- Pages
- 41147-41156
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2067233675
- Cited by
- 377
- References
- 63
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 4 · 2023: 4 · 2022: 13 · 2021: 12 · 2020: 7 · 2019: 14 · 2018: 15 · 2017: 11 · 2016: 8 · 2015: 15 · 2014: 22 · 2013: 13 · 2012: 30
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
- Keywords
- osteoclastogenesis, NFATc1, RANKL, RAW264 cells, gene expression profiling, cyclosporine, tartrate-resistant acid phosphatase, transcriptional regulation, cell differentiation, antisense RNA knockdown
- MeSH terms
- Animals, Base Sequence, Carrier Proteins, Cell Line, DNA-Binding Proteins, Gene Expression Regulation, Glutathione Transferase, Imidazoles, Membrane Glycoproteins, Nuclear Proteins, Osteoclasts, Pyridines, Subcellular Fractions, Transcription Factors, Cyclosporine, DNA Primers, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, NFATC Transcription Factors, Mice, RANK Ligand, Receptor Activator of Nuclear Factor-kappa B
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