Cited by 9,609Gene expression and cancer classificationAdvanced Biosensing Techniques and ApplicationsMolecular Biology Techniques and Applications

Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

Schena, Mark, Shalon, Dari, Davis, Ronald W., Brown, Patrick O.·1995·Science

Abstract

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

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Crossref · 10.1126/science.270.5235.467

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15 of 15 references verified0 citation errors · 0 not checkable

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  • Particularly in the last 25 years, there has been an ever increasing number of ALE experiments with Escherichia coli and Saccharomyces cerevisiae being the most prominent organisms under investigation.

    “however, particularly in the last 25 years, there has been an ever increasing number of such experiments with Escherichia coli and Saccharomyces cerevisiae being the most prominent organisms under investigation [26-28].”

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Authors
Schena, M., Shalon, D., Davis, R. W., & Brown, P. O.
Affiliations
Stanford Medicine (US)
Corresponding author
  • Schena, Mark
  • Shalon, Dari
  • Davis, Ronald W.
  • Brown, Patrick O.
Venue
Science
Published
1995-10-20
Volume
270
Issue
5235
Pages
467-470
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1970156673
Cited by
9,609
References
15
Citations by year
2026: 68 · 2025: 89 · 2024: 117 · 2023: 91 · 2022: 109 · 2021: 137 · 2020: 140 · 2019: 168 · 2018: 193 · 2017: 196 · 2016: 214 · 2015: 269 · 2014: 312 · 2013: 328 · 2012: 418
FWCI
29.38
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r21hg000450-02)
  • National Institute on Aging (R37AG00198)
  • National Human Genome Research Institute (R21HG00450)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene expression and cancer classification
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Keywords
gene expression profiling, differential gene expression, Arabidopsis thaliana, mRNA
MeSH terms
DNA-Binding Proteins, Genetic Techniques, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Messenger, Transcription Factors, RNA Probes, Gene Expression, Genome, Human, Polymerase Chain Reaction, Genes, Plant, Arabidopsis, DNA, Complementary, Homeodomain Proteins, Plant Leaves, Plant Roots, DNA, Plant, RNA, Plant, Arabidopsis Proteins, Plants, Genetically Modified

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