Cited by 285Plant biochemistry and biosynthesisPlant Molecular Biology ResearchLipid metabolism and biosynthesis

Contribution of the Mevalonate and Methylerythritol Phosphate Pathways to the Biosynthesis of Gibberellins inArabidopsis

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Kasahara, Hiroyuki, Hanada, Atsushi, Kuzuyama, Tomohisa, Takagi, Motoki, Kamiya, Yuji, Yamaguchi, Shinjiro·2002·Journal of Biological Chemistry

Abstract

Gibberellins (GAs) are diterpene plant hormones essential for many developmental processes. Although the GA biosynthesis pathway has been well studied, our knowledge on its early stage is still limited. There are two possible routes for the biosynthesis of isoprenoids leading to GAs, the mevalonate (MVA) pathway in the cytosol and the methylerythritol phosphate (MEP) pathway in plastids. To distinguish these possibilities, metabolites from each isoprenoid pathway were selectively labeled with (13)C in Arabidopsis seedlings. Efficient (13)C-labeling was achieved by blocking the endogenous pathway chemically or genetically during the feed of a (13)C-labeled precursor specific to the MVA or MEP pathways. Gas chromatography-mass spectrometry analyses demonstrated that both MVA and MEP pathways can contribute to the biosyntheses of GAs and campesterol, a cytosolic sterol, in Arabidopsis seedlings. While GAs are predominantly synthesized through the MEP pathway, the MVA pathway plays a major role in the biosynthesis of campesterol. Consistent with some crossover between the two pathways, phenotypic defects caused by the block of the MVA and MEP pathways were partially rescued by exogenous application of the MEP and MVA precursors, respectively. We also provide evidence to suggest that the MVA pathway still contributes to GA biosynthesis when this pathway is limiting.

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Crossref · 10.1074/jbc.m208659200

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Authors
Kasahara, H., Hanada, A., Kuzuyama, T., Takagi, M., Kamiya, Y., & Yamaguchi, S.
Affiliations
  • RIKEN (JP)
  • The University of Tokyo (JP)
Corresponding author
Yamaguchi, Shinjiro
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2002-11
Volume
277
Issue
47
Pages
45188-45194
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2024426942
Cited by
285
References
45
Citations by year
2026: 12 · 2025: 10 · 2024: 15 · 2023: 6 · 2022: 17 · 2021: 16 · 2020: 13 · 2019: 8 · 2018: 10 · 2017: 10 · 2016: 11 · 2015: 4 · 2014: 10 · 2013: 16 · 2012: 20
FWCI
3.01
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
Keywords
gibberellin biosynthesis, mevalonate pathway, methylerythritol phosphate pathway, isoprenoid biosynthesis, campesterol, Arabidopsis thaliana, 13C labeling, sterol biosynthesis
MeSH terms
Carbon Isotopes, Erythritol, Gibberellins, Lovastatin, Mevalonic Acid, Pentosephosphates, Plant Growth Regulators, Xylulose, Molecular Structure, Arabidopsis, Seedlings

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