The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Contribution of the Mevalonate and Methylerythritol Phosphate Pathways to the Biosynthesis of Gibberellins inArabidopsis
Open accessAbstract
Gibberellins (GAs) are diterpene plant hormones essential for many developmental processes. Although the GA biosynthesis pathway has been well studied, our knowledge on its early stage is still limited. There are two possible routes for the biosynthesis of isoprenoids leading to GAs, the mevalonate (MVA) pathway in the cytosol and the methylerythritol phosphate (MEP) pathway in plastids. To distinguish these possibilities, metabolites from each isoprenoid pathway were selectively labeled with (13)C in Arabidopsis seedlings. Efficient (13)C-labeling was achieved by blocking the endogenous pathway chemically or genetically during the feed of a (13)C-labeled precursor specific to the MVA or MEP pathways. Gas chromatography-mass spectrometry analyses demonstrated that both MVA and MEP pathways can contribute to the biosyntheses of GAs and campesterol, a cytosolic sterol, in Arabidopsis seedlings. While GAs are predominantly synthesized through the MEP pathway, the MVA pathway plays a major role in the biosynthesis of campesterol. Consistent with some crossover between the two pathways, phenotypic defects caused by the block of the MVA and MEP pathways were partially rescued by exogenous application of the MEP and MVA precursors, respectively. We also provide evidence to suggest that the MVA pathway still contributes to GA biosynthesis when this pathway is limiting.
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“In corn, MVA is not the pathway for synthesizing phytosterols; other pathways participate in synthesizing IPP [18,116,143,144].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [143]
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Cites
Biochemistry and molecular biology of plants
Short, T. W. (2001) · Biochemistry and Molecular Biology Education · Cited by 3,833
Biochemistry & molecular biology of plants
(2001) · Choice Reviews Online · doi:10.5860/choice.38-3895 · Cited by 2,980
THE 1-DEOXY-D-XYLULOSE-5-PHOSPHATE PATHWAY OF ISOPRENOID BIOSYNTHESIS IN PLANTS
Lichtenthaler, H. K. (1999) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.50.1.47 · Cited by 1,420
Rohmer, M., & Rohmer, M. (1999) · Natural Product Reports · doi:10.1039/a709175c · Cited by 1,193
Estévez, J. M., Cantero, A., Reindl, A., Reichler, S. A., & León, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m100854200 · Cited by 672
Sacchettini, J. C., & Poulter, C. D. (1997) · Science · doi:10.1126/science.277.5333.1788 · Cited by 562
The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.
Sun, T., & Kamiya, Y. (1994) · The Plant Cell · doi:10.1105/tpc.6.10.1509 · Cited by 550
Self-citationSilverstone, A. L., Jung, H.-S., Dill, A., Kawaide, H., Kamiya, Y., & Sun, T. (2001) · The Plant Cell · doi:10.1105/tpc.010047 · Cited by 503
Self-citationRohmer, M., Seemann, M., Horbach, S., Bringer-Meyer, S., & Sahm, H. (1996) · Journal of the American Chemical Society · doi:10.1021/ja9538344 · Cited by 484
The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms
Eisenreich, W., Schwarz, M. K., Cartayrade, A., Arigoni, D., Zenk, M. H., & Bacher, A. (1998) · Chemistry & Biology · doi:10.1016/s1074-5521(98)90002-3 · Cited by 437
Research works printed but not linked: 5
- Hanson, 1969, J. Chem. Soc., 981
- Sun, 1994, Plant Cell, 6, 1509
- Silverstone, 2001, Plant Cell, 13, 1555
- Fujioka, 1997, Plant Cell, 9, 1951
- Choe, 1999, Plant Cell, 11, 207
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Tarkowská, D., & Strnad, M. (2016) · Planta · doi:10.1007/s00425-016-2561-z · Cited by 56
Arif, Y., Singh, P., Bajguz, A., & Hayat, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23158664 · Cited by 50
“HE 20-hydroxyecdysone BR brassinosteroid CK cytokinin DMAPP dimethylallyl diphosphate EC ecdysteroid ET ethylene GA gibberellin HPLC high-performance column chromatography IPP isopentenyl diphosphate iPR isopentenyladenosine JA jasmonic acid MDA malondialdehyde MeJA methyl jasmonate MeOH methanol MEP 2C-methyl-erythri…”
BackgroundInfluentialInthima, P., Nakano, M., Otani, M., Niki, T., Nishijima, T., Koshioka, M., & Supaibulwatana, K. (2017) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-017-1171-1 · Cited by 26
Metadata
- Authors
- Kasahara, H., Hanada, A., Kuzuyama, T., Takagi, M., Kamiya, Y., & Yamaguchi, S.
- Affiliations
- RIKEN (JP)
- The University of Tokyo (JP)
- Corresponding author
- Yamaguchi, Shinjiro
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2002-11
- Volume
- 277
- Issue
- 47
- Pages
- 45188-45194
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2024426942
- Cited by
- 285
- References
- 45
- Citations by year
- 2026: 12 · 2025: 10 · 2024: 15 · 2023: 6 · 2022: 17 · 2021: 16 · 2020: 13 · 2019: 8 · 2018: 10 · 2017: 10 · 2016: 11 · 2015: 4 · 2014: 10 · 2013: 16 · 2012: 20
- FWCI
- 3.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- gibberellin biosynthesis, mevalonate pathway, methylerythritol phosphate pathway, isoprenoid biosynthesis, campesterol, Arabidopsis thaliana, 13C labeling, sterol biosynthesis
- MeSH terms
- Carbon Isotopes, Erythritol, Gibberellins, Lovastatin, Mevalonic Acid, Pentosephosphates, Plant Growth Regulators, Xylulose, Molecular Structure, Arabidopsis, Seedlings
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