The DOI matches its Crossref record; 25 of the 25 references it cites were found in a registry or an index.
Identification of a Proline-rich Akt Substrate as a 14-3-3 Binding Partner
Open accessAbstract
Akt (also called protein kinase B) is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, and inhibition of apoptosis as well as transformation of cells. Although a number of cellular proteins have been identified as putative targets of the enzyme, additional substrates may play a role in the varied responses elicited by this enzyme. We have used a combination of 14-3-3 binding and recognition by an antibody to the phosphorylation consensus of the enzyme to identify and isolate one of the major substrates of Akt, which is also a 14-3-3 binding protein. This 40-kDa protein, designated PRAS40, is a proline-rich Akt substrate. Demonstration that it is a substrate of Akt was accomplished by showing that 1) PRAS40 was phosphorylated in vitro by purified Akt on the same site that was phosphorylated in insulin-treated cells; 2) activation of an inducible Akt was alone sufficient to stimulate the phosphorylation of PRAS40; and 3) cells lacking Akt1 and Akt2 exhibit a diminished ability to phosphorylate this protein. Thus, PRAS40 is a novel substrate of Akt, the phosphorylation of which leads to the binding of this protein to 14-3-3.
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Cited by
AKT/PKB signaling: navigating downstream
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mTOR: from growth signal integration to cancer, diabetes and ageing
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Defining the role of mTOR in cancer
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Metadata
- Authors
- Kovacina, K. S., Park, G. Y., Bae, S. S., Guzzetta, A. W., Schaefer, E., Birnbaum, M. J., & Roth, R. A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-03
- Volume
- 278
- Issue
- 12
- Pages
- 10189-10194
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2054683873
- Cited by
- 364
- References
- 25
- FWCI
- 2.14
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Kovacina, K. S., Park, G. Y., Bae, S. S., Guzzetta, A. W., Schaefer, E., Birnbaum, M. J., & Roth, R. A. (2003). Identification of a Proline-rich Akt Substrate as a 14-3-3 Binding Partner. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m210837200
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