The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Human Claspin Is Required for Replication Checkpoint Control
Open accessAbstract
Claspin is a newly identified protein that regulates Chk1 activation in Xenopus. In the present study we investigated the role of human Claspin in the DNA damage/replication checkpoint in mammalian cells. We observed that human Claspin is a cell cycle regulated protein that peaks at S/G2 phase. Claspin localizes in the nuclei, but it only associates with Chk1 following replication stress or other types of DNA damage. In addition, Claspin is phosphorylated in response to replication stress, and this phosphorylation appears to be required for its association with Chk1. Moreover, Claspin interacts with the checkpoint proteins ATR and Rad9. Given that both the ATR and Rad9-Rad1-Hus1 complexes are involved in Chk1 activation, it is possible that Claspin works as an adaptor molecule bringing these molecules together. Using small interfering RNA technology, we have shown that down-regulation of Claspin expression inhibits Chk1 activation in response to replication stress. More importantly, down-regulation of Claspin augments the premature chromatin condensation induced by hydroxyurea, inhibits the UV-induced reduction of DNA synthesis, and decreases cell survival. Taken together, these data imply a potentially critical role for Claspin in replication checkpoint control in mammalian cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Claspin, a Chk1-binding protein, is required for ATR-Chk1 checkpoint activation [127–129].”
From Functional interplay between ATM/ATR-mediated DNA damage response and DNA repair pathways in oxidative stress (2014) · cites this paper as [128]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
Cell cycle checkpoint signaling through the ATM and ATR kinases
Abraham, R. T. (2001) · Genes & Development · doi:10.1101/gad.914401 · Cited by 1,966
Liu, Q., Guntuku, S., Cui, X.-S., Matsuoka, S., Cortez, D., Tamai, K., Luo, G., Carattini-Rivera, S., DeMayo, F., Bradley, A., et al. (2000) · Genes & Development · doi:10.1101/gad.14.12.1448 · Cited by 1,625
Linkage of ATM to Cell Cycle Regulation by the Chk2 Protein Kinase
Matsuoka, S. (1998) · Science · doi:10.1126/science.282.5395.1893 · Cited by 1,322
Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H., & Elledge, S. J. (1997) · Science · doi:10.1126/science.277.5331.1497 · Cited by 1,320
ATR-Mediated Checkpoint Pathways Regulate Phosphorylation and Activation of Human Chk1
Zhao, H., & Piwnica-Worms, H. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.13.4129-4139.2001 · Cited by 1,091
(1999) · PubMed · Cited by 1,030
ATR disruption leads to chromosomal fragmentation and early embryonic lethality
Brown, E. J., & Baltimore, D. (2000) · Genes & Development · doi:10.1101/gad.14.4.397 · Cited by 951
Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro
Matsuoka, S., Rotman, G., Ogawa, A., Shiloh, Y., Tamai, K., & Elledge, S. J. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.190030497 · Cited by 849
Substrate Specificities and Identification of Putative Substrates of ATM Kinase Family Members
Kim, S.-T., Lim, D.-S., Canman, C. E., & Kastan, M. B. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.53.37538 · Cited by 810
Flagged first, then by citations
Research works printed but not linked: 3
- Sarkaria, 1999, Cancer Res., 59, 4375
- Busby, 2000, Cancer Res., 60, 2108
- Guo, 2002, Cell Growth Differ., 13, 77
Cited by
Sorted by citations
Items 201–245 of 245
Keeping RelApse in Chk: molecular mechanisms of Chk1 inhibitor resistance in lymphoma
Black, E. M., Joo, Y. K., & Kabeche, L. (2022) · Biochemical Journal · doi:10.1042/bcj20220461 · Cited by 3
The human homolog of fission yeast Rad17 is implicated in tumor growth
Beretta, G. L., Gatti, L., Cesare, M. D., Corna, E., Tinelli, S., Carenini, N., Zunino, F., & Perego, P. (2008) · Cancer Letters · doi:10.1016/j.canlet.2008.02.057 · Cited by 3
Claspin and Cancer: Where Are We Now?
Azenha, D., & Martins, T. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26188828 · Cited by 2
DNA Damage Sensing and Signaling
Wood, J. L., & Chen, J. (2007) · WORLD SCIENTIFIC eBooks · doi:10.1142/9789812706782_0001 · Cited by 2
Self-citationClaspin is involved in S-phase checkpoint induced by benzo(a)pyrene in 16HBE cells
Qi, Y., Zhao, P., Fu, J., Yao, B., Yuan, Z., Hu, E., & Zhou, Z. (2009) · Toxicology in Vitro · doi:10.1016/j.tiv.2009.05.006 · Cited by 2
Genome-wide association study for carcass traits in Tsarskoye Selo chicken breed
Azovtseva, A. I., Ryabova, A. E., & Dementieva, N. V. (2025) · Ecological genetics · doi:10.17816/ecogen685416 · Cited by 1
Wakabayashi, M., Saijyou, N., Hatakeyama, S., Inoue, H., & Tanaka, S. (2012) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2012.02.007 · Cited by 1
Targeting Myc-driven tumours - BETing on ATR
Muralidharan, S. V. (2016) · Cited by 1
Maintain Genomic Stability: Multitask Of DNA Replication Proteins
Zhu, J. H. W. (2015) · Transcriptomics Open Access · doi:10.4172/2329-8936.1000e108 · Cited by 1
Ling, H. (2011) · Lincoln University Research Archive (Lincoln University) · Cited by 2
Cdc48 targets INQ-localized Mrc1 to facilitate recovery from replication stressPreprint
Colding, C., Autzen, J., Pfander, B., & Lisby, M. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.09.16.460581 · Cited by 1
Cyclin A/CDK2 regulates multiple G2 phase pathways
Oakes, V. (2012)
Involvement of p53 in the S-phase Checkpoint during Nucleotide Deficiencies
Heyer, C. L. (2011) · VCU Scholars Compass (Virginia Commonwealth University) · doi:10.25772/4zf5-2439
Meyerkord, C. L. (2009) · Digital Commons - University of South Florida (University of South Florida)
ATR (ataxia telangiectasia and Rad3 related)
Gagou, M., & Meuth, M. (2011) · Atlas of Genetics and Cytogenetics in Oncology and Haematology · doi:10.4267/2042/44954
Gold, D. A. (2009) · CaltechTHESIS (California Institute of Technology) · doi:10.7907/xg90-5475
McAndrew, C. W., Gastwirt, R. F., & Donoghue, D. J. (2008) · Wiley eBooks · doi:10.1002/9780470048672.wecb057
Alver, B. (2012) · University of Minnesota Digital Conservancy (University of Minnesota)
Combining gemcitabine with checkpoint kinase inhibitors to sensitize pancreatic tumors
Saini, P. (2015) · eDiss (Georg-August-Universität Göttingen) · doi:10.53846/goediss-5028
Butola, Revathi, B., Anila, G., & Kumar, S. (2016) · Research and reviews : journal of chemistry
Identification of Proteins Involved in the Maintenance of Genome Stability
Cheng, Y., & Hang, E. (2015) · TSpace (University of Toronto)
RAD9A Stabilization and Destabilization Processes in Checkpoint Maintenance and Checkpoint Recovery
Zambrano, O., & Frend, W. H. C. (2015) · QSpace (Queen's University Library)
USP9X controls DNA replication fork stability and the replication stress checkpoint through Claspin
McGarry, E. (2016) · Arrow@dit (Dublin Institute of Technology) · doi:10.13025/17576
Characterisation of unessential genes required for survival under conditions of DNA stress
Ezzat, H. A., & Price, C. (2020) · Journal of Genetic Engineering and Biotechnology · doi:10.1186/s43141-020-00025-x
Control of DNA Replication by ATR
Lecona, E., & Fernández-Capetillo, Ó. (2018) · Cancer drug discovery and develogment/Cancer drug discovery and development · doi:10.1007/978-3-319-75836-7_3
The Dynamic Replicon: adapting to a changing cellular environmentPreprint
Herrick, J. (2008) · arXiv (Cornell University) · doi:10.48550/arxiv.0812.4238
Checkpoint defects require WRNIP1 to counteract R-loop-associated genomic instabilityPreprint
Marabitti, V., Lillo, G., Malacaria, E., Palermo, V., Pichierri, P., & Franchitto, A. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/858761
Understanding the biological functions of POPX2 phosphatase through its interactome
Kim, P. (2019) · DR-NTU (Nanyang Technological University) · doi:10.32657/10356/136952
Overview of the DNA Damage Checkpoint
Dupré, A., & Gautier, J. (2007) · Humana Press eBooks · doi:10.1007/978-1-59745-221-2_11
Friedberg, E. C., Walker, G. C., Siede, W., Wood, R. D., Schultz, R. A., & Ellenberger, T. E. (2014) · ASM Press eBooks · doi:10.1128/9781555816704.ch21
Chen, Y., Pereira, E., & Sánchez, Y. (2007) · AfCS-Nature Molecule Pages · doi:10.1038/mp.a000642.01
Impact des R-loops sur le stress réplicatif induit par les oncogènes dans les cellules cancéreuses
Heuzé, J., Lin, Y., Lengronne, A., Poli, J., & Pasero, P. (2023) · Comptes Rendus Biologies · doi:10.5802/crbiol.123-fr
Phosphorylation of Claspin by elF2α kinase protects cells from heat stressPreprint
Yang, C.-C., Goto, H., Oyadomari, S., & Masai, H. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.08.21.670878
Characterisation of UVA-induced DNA damage responses in human skin cells
Conmy, S. A. (2013) · Arrow@dit (Dublin Institute of Technology) · doi:10.13025/16394
Bianco, J. N. (2010) · theses.fr (ABES) · doi:10.70675/c5c5f7fbz5b45z423ezbc8azeb8ce3450ec3
Origins of Cellular Lethality Resulting From a Defect in Homologous Recombination in Human Cells
THOMAS, M. L. (2021) · theses.fr (ABES) · doi:10.70675/294f7c6bz644bz42ddz8fb3zffd32551bc76
Woźny, M., Jastrzębski, J. P., Lewczuk, B., Szyryńska, N., Ziółkowska, N., Florczyk, M., Gomułka, P., Bittner, M., Różyński, R., & Brzuzan, P. (2026) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2026.117934
RTF2 and CLASPIN localize CHK1 to the replication fork to control S-phase progression
Broton, C., Weiner, C. L., Ruiz, P. D., Blobel, N. J., Yao, Y., Conti, B. A., Schmid, E. W., Walter, J. C., & Smogorzewska, A. (2026) · Science Advances · doi:10.1126/sciadv.aec3174
Regulation of TIPIN and replication fork dynamics by USP37
Stromberg, B. R., Scarberry, L., Cho, I. S., & Summers, M. K. (2026) · Molecular Biology of the Cell · doi:10.1091/mbc.e25-08-0391
Metadata
- Authors
- Chini, C. C. S., & Chen, J.
- Affiliations
- Mayo Clinic (US)
- Corresponding author
- Chen, Junjie
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2003-08
- Volume
- 278
- Issue
- 32
- Pages
- 30057-30062
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2137310763
- Cited by
- 244
- References
- 39
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 2 · 2023: 9 · 2022: 2 · 2021: 9 · 2020: 8 · 2019: 13 · 2018: 5 · 2017: 8 · 2016: 12 · 2015: 11 · 2014: 8 · 2013: 10 · 2012: 15
- FWCI
- 5.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Mayo Clinic
- National Institutes of Health
- National Cancer Institute (R01CA089239, R01 CA 89239, R01CA092312, CA92312)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- Claspin, S-phase checkpoint, DNA damage checkpoint, Chk1 activation, ATR, protein phosphorylation, replication stress, cell cycle regulation, chromatin condensation, hydroxyurea, adaptor proteins
- MeSH terms
- Carrier Proteins, Cell Cycle, Cell Line, Cell Survival, Chromatin, DNA, DNA Damage, DNA-Binding Proteins, Endonucleases, HeLa Cells, Humans, Hydroxyurea, Microscopy, Fluorescence, Mitosis, Phosphorylation, Precipitin Tests, Time Factors, Transfection, Tumor Cells, Cultured, Ultraviolet Rays, Down-Regulation, G2 Phase, S Phase, Protein Serine-Threonine Kinases, Cell Cycle Proteins, K562 Cells, Schizosaccharomyces pombe Proteins, Xenopus Proteins, RNA, Small Interfering, Adaptor Proteins, Signal Transducing, Ataxia Telangiectasia Mutated Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
