CoChat
Sign Up
Cited by 194

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Mass Spectrometric and Mutational Analyses Reveal Lys-6-linked Polyubiquitin Chains Catalyzed by BRCA1-BARD1 Ubiquitin Ligase

Nishikawa, Hiroyuki, Ooka, Seido, Sato, Ko, Arima, Kei, Okamoto, Joji, Klevit, Rachel E., Fukuda, Mamoru, Ohta, Tomohiko·2004·Journal of Biological Chemistry
DOI 10.1074/jbc.m308540200
Open this paper in CoChat — free account

Abstract

The breast and ovarian cancer suppressor BRCA1 acquires significant ubiquitin ligase activity when bound to BARD1 as a RING heterodimer. Although the activity may well be important for the role of BRCA1 as a tumor suppressor, the biochemical consequence of the activity is not yet known. Here we report that BRCA1-BARD1 catalyzes Lys-6-linked polyubiquitin chain formation. K6R mutation of ubiquitin dramatically reduces the polyubiquitin products mediated by BRCA1-BARD1 in vitro. BRCA1-BARD1 preferentially utilizes ubiquitin with a single Lys residue at Lys-6 or Lys-29 to mediate autoubiquitination of BRCA1 in vivo. Furthermore, mass spectrometry analysis identified the Lys-6-linked branched ubiquitin fragment from the polyubiquitin chain produced by BRCA1-BARD1 using wild type ubiquitin. The BRCA1-BARD1-mediated Lys-6-linked polyubiquitin chains are deubiquitinated by 26 S proteasome in vitro, whereas autoubiquitinated CUL1 through Lys-48-linked polyubiquitin chains is degraded. Proteasome inhibitors do not alter the steady state level of the autoubiquitinated BRCA1 in vivo. Hence, the results indicate that BRCA1-BARD1 mediates novel polyubiquitin chains that may be distinctly edited by 26 S proteasome from conventional Lys-48-linked polyubiquitin chains.

Overview

Integrity signals

Registry

Crossref · 10.1074/jbc.m308540200

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Show all 3 references

Research works printed but not linked: 32

  1. Venkitaraman, 2002, Cell, 108, 171, 10.1016/S0092-8674(02)00615-3 · DOI · Paper page
  2. Moynahan, 2002, Oncogene, 21, 8994, 10.1038/sj.onc.1206177 · DOI
  3. Jasin, 2002, Oncogene, 21, 8981, 10.1038/sj.onc.1206176 · DOI
  4. Chen, 2002, J. Biol. Chem., 277, 22085, 10.1074/jbc.M201252200 · DOI
  5. Xia, 2003, J. Biol. Chem., 278, 5255, 10.1074/jbc.M204591200 · DOI
  6. Mallery, 2002, EMBO J., 21, 6755, 10.1093/emboj/cdf691 · DOI
  7. Kentsis, 2002, Proc. Natl. Acad. Sci. U. S. A., 99, 15404, 10.1073/pnas.202608799 · DOI · Paper page
  8. Baer, 2002, Curr. Opin. Genet. Dev., 12, 86, 10.1016/S0959-437X(01)00269-6 · DOI
  9. Hershko, 1998, Annu. Rev. Biochem., 67, 425, 10.1146/annurev.biochem.67.1.425 · DOI · Paper page
  10. Pickart, 2001, Mol. Cell, 8, 499, 10.1016/S1097-2765(01)00347-1 · DOI
  11. Johnson, 1995, J. Biol. Chem., 270, 17442, 10.1074/jbc.270.29.17442 · DOI
  12. Galan, 1997, EMBO J., 16, 5847, 10.1093/emboj/16.19.5847 · DOI
  13. Deng, 2000, Cell, 103, 351, 10.1016/S0092-8674(00)00126-4 · DOI · Paper page
  14. Wang, 2001, Nature, 412, 346, 10.1038/35085597 · DOI · Paper page
  15. Spence, 2000, Cell, 102, 67, 10.1016/S0092-8674(00)00011-8 · DOI
  16. Spence, 1995, Mol. Cell. Biol., 15, 1265, 10.1128/MCB.15.3.1265 · DOI
  17. Hofmann, 1999, Cell, 96, 645, 10.1016/S0092-8674(00)80575-9 · DOI
  18. Hoege, 2002, Nature, 419, 135, 10.1038/nature00991 · DOI
  19. Wu-Baer, 2003, J. Biol. Chem., 278, 34743, 10.1074/jbc.C300249200 · DOI
  20. Ohta, 1999, Mol. Cell, 3, 535, 10.1016/S1097-2765(00)80482-7 · DOI
  21. Treier, 1994, Cell, 78, 787, 10.1016/S0092-8674(94)90502-9 · DOI
  22. Brzovic, 2003, Proc. Natl. Acad. Sci. U. S. A., 100, 5646, 10.1073/pnas.0836054100 · DOI · Paper page
  23. Ruffner, 2001, Proc. Natl. Acad. Sci. U. S. A., 98, 5134, 10.1073/pnas.081068398 · DOI
  24. Baboshina, 1996, J. Biol. Chem., 271, 2823, 10.1074/jbc.271.5.2823 · DOI
  25. Lam, 1997, J. Biol. Chem., 272, 28438, 10.1074/jbc.272.45.28438 · DOI
  26. Coux, 1996, Annu. Rev. Biochem., 65, 801, 10.1146/annurev.bi.65.070196.004101 · DOI
  27. Verma, 2002, Science, 298, 611, 10.1126/science.1075898 · DOI
  28. Yao, 2002, Nature, 419, 403, 10.1038/nature01071 · DOI
  29. Lam, 1997, Nature, 385, 737, 10.1038/385737a0 · DOI
  30. Holzl, 2000, J. Cell Biol., 150, 119, 10.1083/jcb.150.1.119 · DOI
  31. Joukov, 2001, Proc. Natl. Acad. Sci. U. S. A., 98, 12078, 10.1073/pnas.211427098 · DOI
  32. Peng, 2003, Nat. Biotechnol., 21, 921, 10.1038/nbt849 · DOI

Cited by

Sorted by citations

See all 194

Metadata

Authors
Nishikawa, H., Ooka, S., Sato, K., Arima, K., Okamoto, J., Klevit, R. E., Fukuda, M., & Ohta, T.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2004-02
Volume
279
Issue
6
Pages
3916-3924
ISSN
0021-9258
DOI
10.1074/jbc.m308540200
Cited by
194
References
3
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review